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A Highly Sensitive Spectrophotometric Method for the Micro level Determination of Nitrite and Nitrate anions by Coupling of Tetrazotized Benzidine and N,N-dimethylaniline

Author Affiliations

  • 1Department of Chemistry, Srinivas Institute of Technology, Merlapadav, Mangaluru-574 143, INDIA
  • 2 Department of Chemistry, Dr. M.V Shetty Institute of Technology, Moodbidri-574 225, INDIA

Res.J.chem.sci., Volume 5, Issue (2), Pages 42-47, February,18 (2015)


A simple, sensitive and rapid spectrophotometric method for the determination of anions, nitrite and nitrate has been described. Determination of nitrite is by means of reactions involving tetrazotisation of Benzidine, followed by coupling withN,N-dimethylaniline and determination of nitrate is mainly based on reduction of nitrate to nitrite by using granular Zinc in NaCl. The produced nitrite is then availed to tetrazotize the Benzidine, which is then coupled with N,N-dimethylaniline to form pinkish red colored an azo dye in acidic medium. Measured the absorbance of resulted colored dye solution at 538 nm. Amount of reagents required is optimised and the extent of tolerance limit by the diverse ions was investigated. The Beer-Lambertís linearity range for Benzidine- N,N-dimethylaniline couple was found to be 0.05 to 0.3g/mL of nitrite with molar absorptivity 3.475x10Lmol-1cm-1 and sandellís sensitivity 3.3152x10-4gcm-2. The evaluated detection limit and quantization limit for the determination of nitrite were 0.00348gmL-1 and 0.0105 gmL-1 respectively. The investigated method is useful for the determination of microgram quantity of anions nitrite and nitrate in water and soil specimens.


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