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Spectrophotometric Analysis of Chlorophylls and Carotenoids from Commonly Grown Fern Species by Using Various Extracting Solvents

Author Affiliations

  • 1Department of Basic Science, EIILM University, Sikkim, INDIA
  • 2Department of Biotechnology and Environmental Science, EIILM University, Sikkim, INDIA
  • 3Department of Botany, North Bengal University, West Bengal, INDIA
  • 4Department of Chemistry, Malda Government Polytechnic College (under DTET-WB), West Bengal, INDIA

Res.J.chem.sci., Volume 4, Issue (9), Pages 63-69, September,18 (2014)


Present investigation is performed on the comparative extraction of photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) by using solvents of different chemical nature. The study is also concern on the extraction ratio of biomolecules with respect to time duration/variation. Different trend is observed in extraction rate for chlorophylls and carotenoids. Highest extraction of chlorophylls (Ch-a and Ch-b) is noted for DEE (except chlorophyll b in Adiantum sp.). Whereas maximum extraction of caroteniods is performed by acetone in Adiantum sp., and for Crystiella sp. and Drypteris sp DMSO execute best extraction of carotenoids. Significant variations (p <0.01) in pigment concentrations are also noted for sampled species exposed to different chemical extractant solvents for different time duration.


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