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Development of Electrochemical sensor based on Poly (xylenol orange) film towards the determination of L-Dopa and its simultaneous resolution in the presence of Uric acid: A cyclic Voltammetric study

Author Affiliations

  • 1 Electrochemical Research Laboratory, Department of Chemistry, Sri Venkateswara University, Tirupati- 517 502, Andhra Pradesh, INDIA

Res.J.chem.sci., Volume 4, Issue (4), Pages 37-43, April,18 (2014)


The surface of carbon paste electrode (CPE) was modified based on electropolymerization of xylenol orange (XO) using cyclic voltammetry. This poly (XO) modified carbon paste electrode (poly(XO)MCPE) exhibited good electrocatalytic activity towards the quantification of L-dopa in 1mM phosphate buffer solution (PBS) at pH 5.0. The method was used to study the factors such as effects of pH,scan rate and concentration. Kinetic parameters such as diffusion coefficient (D), area of the modified electrode (A), limit of detection and limit of quantification of L-dopa were calculated. Simultaneous determination of L-dopa in the presence of uric acid (UA) was studied by differential pulse voltammetry (DPV). The interfacial electron transfer behaviour of L-dopa was studied by means of electrochemcal impedance spectroscopy (EIS). The practical analytical application of poly(XO)MCPE towards the detection of L-dopa in a commonly available commercial tablet sample has also been evaluated.


  1. Khor S., Hsu A., The pharmacokinetics and pharmacodynamics of levodopa in the treatment of Parkinson's disease, Curr. Clin. Pharmacol.,2(3), 234-243(2007)
  2. Honda H., Gindin R.A.,Gout while receiving levodopa for parkinsonism, J. Am.Med. Assoc., 219, 55–57 (1972)
  3. Katzenschlager R., Lees A.J., Treatment of Parkinson's disease: levodopa as the first choice, J. Neurol., 249, II/19–II/24 (2002)
  4. Weiner W.J., Diagnosis & Clinical Mangement, Demos Medical Publishing, New York, 2002
  5. Nematollahi D., Rafiee M., Fotouhi L., Mechanistic study of homogeneous reactions coupled with electrochemical oxidation of catechols,J. Iran. Chem. Soc., 6, 448–476 (2009)
  6. Melamed.E, Offen.D, Shirvan.A., Ziv.I.,Levodopa-an exotoxin or a therapeutic drug?, J. Neurol.,247, 135–139 (2000)
  7. Popa.E, Kubota.Y, D.A. Tryk, A. Fujishima, Selective voltammetric and amperometric detection of uric acid with oxidized diamond film electrodes, Anal.Chem., 72, 1724–1727 (2000)
  8. Hansson.C., Agrup.G., Rorsman.H., Rosengren.A.M., Analysis of cysteinyldopas, dopa, dopamine, noradrenaline and adrenaline in serum and urine using high-performance liquid chromatography and electrochemical detection, J. Chromatogr., 162, 7–22 (1979)
  9. Cannazza.G., Di Stefano.A., Mosciatti.B., Braghiroli.D., M. Baraldi, F. Pinnen, P.Sozio, C.Benatti, C. Parenti, Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of -dopa prodrugs by mean of HPLC–EC, J. Pharmaceut. Biomed. Anal.36, 1079–1084 (2005)
  10. Helaleh M.I.H., Rahman N., Abu-Nameh E.S.M.,Use of cerium(iv) nitrate in the spectrophotometric determination of levodopa and methyldopa in the pure form and pharmaceutical preparations, Anal. Sci., 13, 1007–1010 (1997)
  11. Teixeira M.F.S., Marcolino-J´unior L.H., Fatibello-Filho O., Dockal E.R., Bergamini M. F., An electrochemical sensor for -dopa based on oxovanadium-salen thin film electrode applied flow injection system, Sens. Actuat. B,122(2), 549–555 (2007)
  12. He W.W., Zhou X.W., Lu J.Q., Simultaneous determination of benserazide and levodopa by capillary electrophoresis–chemiluminescence using an improved interface, J. Chromatogr. A,1131, 289-292 (2006)
  13. Marques K.L., Santos J.L.M., Lopes J.A., Lima J.L.F.C., Simultaneous chemiluminometric determination of levodopa and benserazide in a multi-pumping flow system with multivariate calibration, Anal. Sci., 24, 985-991(2008)
  14. Wring S.A., Hart J.P., Chemically modified, carbon-based electrodes and their application as electrochemical sensors for the analysis of biologically important compounds, Analyst, 117,1215-1229 (1992)
  15. Yan X.X., Pang D.W., Lu Z.X., Lü J.Q., Tong H., Electrochemical behavior of -dopa at single-wall carbon nanotube-modified glassy carbon electrodes, J. Electroanal. Chem.,569, 47-52 (2004)
  16. Hua G.Z., Chen L., Guo Y., Wang X.L., Shao S.J, Selective determination of L-dopa in the presence of uric acid and ascorbic acid at a gold nanoparticle self-assembled carbon nanotube-modified pyrolytic graphite electrode, Electrochim. Acta., 55, 4711-4716 (2010)
  17. Reddaiah K., Reddy T.M., Raghu P., Electrochemical investigation of L-dopa and simultaneous resolution in the presence of uric acid and ascorbic acid at a poly (methyl orange) film coated electrode: A voltammetric study, J. Electroanal. Chem.,682, 164-171 (2012)
  18. Ohnuki Y., matsuda H., Ohsaka T., Oyama N., Permselectivity of films prepared by electrochemical oxidation of phenol and amino-aromatic compounds, J. Electroanal. Chem.,158, 55-67 (1983)
  19. Cai C.X., Xue K.H., Electrochemical characterization of electropolymerized film of Naphthol-green-B and its electrocatalytic activity toward NADH oxidation, Microchem J., 58, 197-208 (1998)
  20. Karyakin A.A., Bobrova O.A., Karyakina E.E., Electroreduction of NAD to enzymatically active NADH at poly(neutral red) modified electrodes, J. Electroanal. Chem., 399, 179-184 (1995)
  21. Wang B.Q., Li B., Wang Z.X., Xu G.B., Wang Q., Dong S.J., Sol-Gel thin-film immobilized Soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium, Anal. Chem., 71, 1935-1939 (1999)
  22. Nicholson R.S., Shain I., Theory of stationary electrode polarography: Single scan and cyclic methods applied to reversible, irreversible and kinetic systems, Anal.Chem.,36, 706-723 (1964)
  23. Raghu P., Swamy B.E.K., Reddy T.M., Chandrashekar B.N., Reddaiah K., Sol–gel immobilized biosensor for the detection of organophosphorous pesticides: A voltammetric method, Bioelectrochemistry,83, 19-24 (2012)
  24. Reddaiah K., Reddy M.M., Raghu P., Reddy T.M., An electrochemical sensor based on poly (solochrome dark blue) film coated electrode for the determination of dopamine and simultaneous separation in the presence of uric acid and ascorbic acid: A voltammetric method, Colloids Surf. B, 106, 145-150 (2013)
  25. Viswanathan S., ChinLiao W., Huang C.C., Rapid analysis of L-dopa in urine samples using gold nanoelectrode ensembles, Talanta, 74, 229-234 (2007)
  26. Raghu P., Reddy T.M., Swamy B.E.K., Chandrashekar B.N., Reddaiah K., Development of AChE biosensor for the determination of methyl parathion and monocrotophos in water and fruit samples:A cyclic voltammetric study, J. Electroanal. Chem.,665, 76-82 (2012)
  27. Chandra U., Swamy B.E.K., Gilbert O., Sherigara B.S., Voltammetric resolution of dopamine in the presence of ascorbic acid and uric acid at poly (calmagite) film coated carbon paste electrode, Electrochim. Acta,55, 7166-7174 (2010)
  28. Gopal P., Reddy T.M., Reddaiah K., Raghu P., Narayana P.V., An electrochemical investigation and reduction mechanism of 3,5-Dinitrobenzoic acid at a glassy carbon electrode: A voltammetric study, J. Mol. Liq., 178, 168-174 (2013)
  29. Atta F.N., Galal A.A., Ahmed R., Poly(3,4-ethylene-dioxythiophene) electrode for the selective determination of dopamine in presence of sodium dodecyl sulfate, Bioelectrochemistry,80, 132–141 (2011)