International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Phytochemical Analysis of Stylosanthes fruticosa using UV-VIS, FTIR and GC-MS

    , ,

Author Affiliations

  • 1Department of Chemistry, St. Joseph’s college, Cuddalore, Tamilnadu, INDIA
  • 2Department of chemistry, St. Joseph’s College, Thiruchirappalli, Tamilnadu, INDIA

Res.J.chem.sci., Volume 3, Issue (11), Pages 14-23, November,18 (2013)

Abstract

The present study was carried out to characterize the bioactive constituents present in different leaf extracts of Stylosanthes fruticosa using UV-VIS, FTIR and GC-MS. The crude extracts were scanned in the wavelength ranging from 200-1100nm by using Perkin Elmer spectrophotometer and the characteristic peaks were detected. For GC-MS analysis,10 g sample is extracted with 30 ml ethanol, filtered in ash less filter paper with 2 g sodium sulphate and the extract is concentrated to 1 ml by bubbling nitrogen into the solution. The compound detection employed the NIST Ver.2.0 Year 2005 library. The biological activities are based on Dr. Duke’s phytochemical and ethnobotanical Databases by Dr. Jim Duke of the Agricultural Research Service/USDA. The UV-VIS profile showed different peaks ranging from 400-700 nm with different absorption respectively. The FTIR spectrum confirmed the presence of secondary alcohols, phenols, alkanes, alkenes, carboxylic acids, aromatics, nitro compounds and amines in different extracts. The results of the GC-MS analysis provide different peaks determining the presence of 21 phytochemical compounds with different therapeutic activities. The major phyto constituents were Trans-5-Hexyl-1,4-dioxane-2-carboxylic acid (9.26%), dodecadienoic acid, Methy-ester(6.58%) and Nonanoic acid Methyl ester (6.58%). Hence, this study offers base of using as herbal alternative for the synthesis of antimicrobial agents.

References

  1. Garro L.C., Intercultural variation folk medicinal knowledge: A comparison between curers and noncurers, American anthropologist, 88, 351370 (1986)
  2. Parekh J. and Chanda V., In vitro Antimicrobial activity and Phytochemical analysis of some Indian medicinal plants, Turkish J. Biol.,31, 5358 (2007)
  3. Eisenhauer N., Klier M., Partsch S., Sabais A.C.W., Scherber C., Weisser W. and Scheu S., No interactive effects of pesticides and plant diversity on soil microbial biomass and respiration, Appl. Soil Ecol., 42, 3136 (2009)
  4. Uzer A., Ercag E. and Apak R., Selective spectrophotometric determination of TNT in soil and water with dicyclohexylamine extraction, Anal.Chim.Acta,534, 307317 (2005)
  5. Pico Y. and Kozmutza C., Evaluation of pesticide residue in grape juices and the effect of natural antioxidants on their degradation rate, Anal.Bioanal.Chem.,389, 18051814 (2007)
  6. Khalaf N.A., Shakya A.K., Al-othman A., Ahbar Z. and Farah H., Antioxdidant activity of some common plants, Turk. J. Biol., 31, 1-5 (2007)
  7. Nair R. and Chanda S., Activity of some medicinal plants against certain pathogenic bacterial strains, Indian J. Pharmacol., 38, 142-144 (2006)
  8. Nascimento G.G.F., Locatelli J., Freitas P.C. and Silva G.L., Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria, Braz. J. Microbiol., 31, 247-256 (2000)
  9. Mathekaga A.D. and Meyer J.J.M., Antibacterial activity of South African Helichrysumspecies, South Afr. J. Bot., 64, 293-295 (1998)
  10. Uzer A., Ercag E. and Apak R., Selective spectrophotometric determination of TNT in soil and water with dicyclohexylamine extraction, Anal.Chim.Acta,534, 307-317 (2005)
  11. Eisenhauer N., Klier M., Partsch S., Sabais A.C.W., Scherber C., Weisser W. and Scheu S., No interactive effects of pesticides and plant diversity on soil microbial biomass and respiration, Appl. Soil Ecol., 42, 31-36 (2009)
  12. Liebler D.C., Burr J.A., Philips L. and Ham A.J.L., Gas chromatography-mass spectrometry analysis of vitamin E and its oxidation products, Anal.Biochem., 236, 27-34 (1996)
  13. Aysal P., Ambrus A.D., Lehotay S.J. and Cannavan A., Validation of an efficient method for the determination of pesticide residues in fruits and vegetables using ethyl acetate for extraction, J. Environ. Sci. Heal., 42, 481-490 (2007)
  14. Ibrahim M. and Abd-El-Aal M., Spectroscopic study of Heavy Metals Interaction with Organic Acid, Int. J. Environment and Pollution, 35(1), 99-110 (2008)
  15. Ibrahim M., Hameed A.J. and Jalbout A., Molecular Spectroscopic Study of River Nile Sediment in the Greater Cairo Region, Applied Spectroscopy, 62(3), 306-311 (2008)
  16. Indian Medicinal Plants, Orient Longman, Chennai, 5, 352 (2004)
  17. Kapoor L.D., Ayurvedic Medicinal Plants; Edn 1, CRC Press, Mumbai, 2-4 (2005)
  18. Fernie A.R., Trethewey R.N. and Krotzky A.J., Innovation–Metabolite profiling: from diagnostics to systems biology, Nat. Rev. Mol. Cell. Biol, , 763-769 (2004)
  19. Robertson D.G., Metabolomics in toxicology: A review, Toxicol Sci, 85, 809-822 (2005)
  20. Merlin N.J., Parthasarathy V., Manavalan R. and Kumaravel S., Chemical Investigation of Aerial Parts of Gmelinaasiatica Linn by GC-MS, Pharmacognosy Res, 1(3), 152-156 (2009)
  21. Komal Kumar J. and Devi Prasad A.G., Identification and comparison of biomolecules in medicinal plants of Tephrosiatinctoria and Atylosiaalbicansby using FTIR, Romanian J. Biophys, 21(1), 63-71 (2011)
  22. Sofowara A., Medicinal plants and Traditional medicine in Africa, Spectrum Books Ltd, Ibadan, Nigeria, 289 (1993)
  23. Gills L.S., Ethnomedical uses of plants in Nigeria, University of Benin press, Nigeria, 276 (1992)
  24. Taylor-Smith R., Investigations on plants of West Africa, 111 Phytochemical Studies of some plants of Sierra Leone, Bull. Insst. France Afn noire A, 28, 528-541 (1966)