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Volatile oil composition and antiproliferative activity of Hyptis spicigera Lam against human breast adenocarcinoma cells MCF-7

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Author Affiliations

  • 1Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA) EPAC/UAC, 01 BP 2009 Cotonou, BENIN
  • 2Herbier National, Département de Botanique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, BENIN
  • 3Clermont Université, ENSCCF, Institut de Chimie de Clermont-Ferrand, BP10448, F-63000 Clermont-Ferrand, FRANCE
  • 4CNRS, UMR 6296, ICCF, F-63177 Aubière, FRANCE
  • 5Université d’Auvergne-Faculté de Pharmacie, Laboratoire des Sciences Végétales et Fongiques pharmaceutiques, Equipe EA4233 «Nutrition, Cancérogenèse et Thérapie antitumorale», FRANCE
  • 6LEXVA Analytique, 460 rue du Montant, 63110 Beaumont, FRANCE

Res.J.chem.sci., Volume 3, Issue (1), Pages 27-31, January,18 (2013)

Abstract

This study reported the chemical composition and the evaluation of the in vitro antiproliferative activity of volatile extracts of Hyptis spicigera (HS) on breast cancer cells MCF-7. Essential oils (EO) HS1 and HS2 extracted from leafy stems of H. spicigera by hydrodistillation after different harvesting periods were characterized by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS). The cytotoxicity of these volatile extracts was tested in order to assess their antiproliferative activity on cancer cells MCF-7 by the resazurin test. Chromatographic analysis revealed several major compounds depending on the harvest period of vegetable. Sample HS1 was characterized by -pinene (16.9%), sabinene (13.8%), β-pinene (9.6%) and 1,8-cineole (9.6%), while HS2 was potentially rich in β-caryophyllene (53.2%), caryophyllene oxide (5.4%) and α-humulene (3.8%). Biological investigations performed have shown that HS1 and HS2 were cytotoxic against MCF-7 cells. Inhibitory concentrations values (IC50) determined for this purpose were 170 and 84 μg/mL respectively for HS1 and HS2. Major compounds of the EO should be responsible for this inhibitory activity.

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