International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Studies on Antivenom Activities of Ficus Iteophyla MIQ and Borassus Aethiopum Plant Extracts against Naja Mossandica Snake Venom

    , ,

Author Affiliations

  • 1Biochemistry/Chemistry section, Department of Applied Science, Kaduna Polytechnic, Kaduna, NIGERIA
  • 2Biology/Microbiology Department of Applied Science, Kaduna Polytechnic, Kaduna, NIGERIA

Res.J.chem.sci., Volume 2, Issue (9), Pages 1-4, September,18 (2012)

Abstract

The antivenom properties of methanolic extract of Ficus iteophylla miq and Borassus aethiopum plants were investigated against the venom of Naja mossandica snake. Both plant extracts effectively reduced the Naja mossandica venom induced hemolytic activity. The result reveals that hemolysis due to venom was 81.5% without extract but the heomolytic activity was drastically reduced by Ficus iteophylla miq and Borassus aethiopum plant extracts to 41.9% and 61.7% respectively. Pharmacological activities like coagulant, packed cell volume (PCV) and phospholipase A2 were significantly neutralized by both plants extracts. The activity of phospholipase A decreases from 600µmol/min to 267mol/min and 400mol/min by the Ficus iteophylla miq and Borassus aethiopum plant extracts respectively. The investigations revealed that Ficus iteophylla miq and Borassus aethiopum plant extracts posses potent snake venom neutralizing activity. This finding will provide an alternative ways to inhibit venom toxins in snakebite victims and may provide supplemental treatments to serum therapy.

References

  1. Paramonte B., Snake bites in Nigeria, medicine journal of therapeutic Africa, University of the Science in Philadalphia USA, 1(3), 222-227 (2007)
  2. Sakai A, (clinical feature of envenomation bythe snake, Yamakagashi Rhabdophis tigrinus), Chudoka kenkyu, 20(3), 235-243 (2007)
  3. Maya Devi C., Vasantha Bai, Mary Vijayan Lal, Arthur Umashankar, Krishnan P.R. and Lissy K., An improved method for isolation of antiviper venom antibodies from chicken egg yolk-journal of Biochemical and Biophysical, (2002)
  4. Pereira N.A., Parina B.M., Do-Nasamento C.M., Pevente J.P. and More W.B., PharmacoIogical Screening of plants recommended by folk medicina as snake venom antitode, Plant Med., 60(2), 99-100 (1994)
  5. Alam M.I., Audy B. and Gomes A., Viper venom neutralization by medical plants Hemidesmus indicus and pluchae indica root extract, phytotherapy Research, 10, 58 (1996)
  6. Chatterje I., Chakrayarty A.K. and Gomes A., Antisnake venom activity of ethanolic seed extract of strychnos nux vomica linn, Indian Journal of experimental Biology, 42, 468-475 (2004)
  7. Dougterty W.M., Introduction to hematology (Second edition), The C.V. Mosby LT. Lovis U.S.A., 240-253 (1976)
  8. Simuzu S., Tan I.Y., Yamada H., Tabata M., and Murachi I., Anal Biochem., 107, 193-198 (1980)
  9. Theakston R.D.G. and Reid H.A., Development of simple standard assay procedures for the characterization of snake venoms Bulletin of Organization the world Health, 61, 949-956 (1983)
  10. Coles E.M., Veterinary clinical pathology (3rd edition) W.B. Samder comp, London, 29-75 (1980)
  11. Lakhotia Manoj, Kothari Dinesh, Choudhary Dharma Ram, Sharma Sanjeev and Pravesh Jain, A case saw scale viper snake bite presenting as pleuro-peicardial Heremorhage, JIACM, 3(4), 392.4 (2002)
  12. Santosh R.J. Fattepur and Shivaji and Gawade P., Prelimmary screening of Herbal plant Extracts for Anti-venom activity against common sea snake (Enhydrina Schistosa) poisoning pharmacognosy magazine 16, 56-60 (2004)
  13. Alam M.I. and Gomes A., Snake venom neutralization by Indian medicinal plants (vitex negundo and Emblica officialis) root extracts, Journal of Ethnopharmacology, 86, 75–80 (2003)
  14. Yao-ching Hung, Vasylsava, Meng-Yenttong, G. Stephen Huang, Inhibitory effects on phospholipase A2 and antivenom activity of melanim from extracted Thea sinensis Linn., Life sciences, 74, 2037-2047 (2004)