International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System

Author Affiliations

  • 1Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli- 620 015, INDIA

Res.J.chem.sci., Volume 2, Issue (7), Pages 47-52, July,18 (2012)


Shrimp waste is an important source of bioactive molecules and it undergoes rapid disintegration which leading to environmental pollution. It is necessary to preserve the material adopting the environmentally safe techniques, prior to recovery of bioactive components such as proteins and carotenoids. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. Hence, a feasible protocol for the recovery of protein from Shrimp Waste has been established. The best conditions of partitioning were achieved using ATPS composed of PEG 4000, ammonium citrate salt, pH8, 1M addition of sodium chloride, and 36.28 Tie line length. The maximum percentage yield of protein extracted from shrimp waste was found to be 74.50%.


  1. The Marine Products Export Development Authority, India, Available from http://
  2. Ekpete O.A., Horsfall M. JNR., Preparation and Characterization of Activated Carbon derived from Fluted Pumpkin Stem Waste (Telfairia occidentalis Hook F), Res.J.Chem.Sci. 1, 10-17 (2011)
  3. Shabudeen P.S. Syed,Study of the Removal of Malachite Green from Aqueous Solution by using Solid Agricultural Waste, Res.J.Chem.Sci.,1, 89-104(2011)
  4. Sachindra N.M., Bhaskar N. and Mahendrakar N.S., Recovery of carotenoids from shrimp waste in organic solvents, Waste Manage, 26, 1092–1098 (2006)
  5. Barratt A. and Montano R., Shrimp heads - a new source of protein, Infofish Marketing Digest 4(86), 21-22 (1986)
  6. Sachindra N.M. and Bhaskara N., In vitro antioxidant activity of liquor from fermented shrimp bio Waste, Bioresource Technol, 99, 9013–9016 (2008)
  7. Handayani A.D., Indraswati S.N. and Ismadji S, Extraction of astaxanthin from giant tiger (Panaeus monodon) shrimp waste using palm oil:studies of extraction kinetics and thermodynamic, Bioresource Technol, 99, 4414–4419 (2008)
  8. Redde R.H., Einbu A. and Varum K.M., A seasonal study of the chemical composition and chitin quality of shrimp shells obtained from northern shrimp (Pandalus borealis), Carbohydr Polym., 71, 388-393 (2008)
  9. Hur J.W. and No H.K., Physicochemical properties of chitin and chitosan prepared from lobster shrimp shell, Korean J. Food Sci. Tech,28, 870–876 (1996)
  10. Coward-Kelly G., Agbogbo F.K. and Holtzapple M.T., Lime treatment of shrimp head waste for the generation of highly digestible animal feed, Bioresource Technol, 97, 1515–1520 (2006)
  11. Schugerl K. and Hubbuch J., Integrated bioprocesses, Curr. Opin. Microbiol., 8, 294–300 (2005)
  12. Nucci H.D., Nerli B. and Pico G., Comparison between the thermodynamic features of -antitrypsin and human albumin partitioning in aqueous two-phase systems of polyethyleneglycol –dextran, Biophys. Chem., 89 219–229 (2001)
  13. Salabat, The influence of salts on the phase compositions in aqueous two-phase systems: experiments and predictions, Fluid Phase Equilibr, 187–188, 489–498 (2001)
  14. Hatti-Kaul R., Methods in Biotechnology, Aqueous Two-Phase Systems, Methods and Protocols, Humana Press, New Jersey, (2000)
  15. Tubio G., Pellegrini L., Nerli B.B. and Pico G.A., Liquid – liquid Equlibria of aqueous two-phase systems containing poly (ethylene glycol) of different molecular weight and sodium citrate, J Chem Eng Data, 51, 209–212 (2006)
  16. Albertsson P.A., Cajarville A., Brooks D.E. and Tjerneld F., Partition of proteins in aqueous two-phase systems and the effect of molecular mass of the polymer, Biochem Biophys Acta, 926, 87–93 (1987)
  17. Pico G., Romanini D., Nerli B. and Farruggia B., Polyethylene glycol molecular mass and polydispersivity effect on protein partitioning in aqueous two-phase systems, J Chromatogr B., 830, 286–292 (2006)
  18. Rito-Palomares M., Practical application of aqueous two phase partition to process development for the recovery of biological products, J Chromatogr B., 807, 3–11 (2005)
  19. Schmidt A.S., Ventom A.M. and Asenjo J.A., Partitioning and purification of -amylase in aqueous two-phase systems, Enzyme Microb. Technol., 16, 131–142 (1994)
  20. Reghetti P.G. and Caravaggio T., Isoelectric points and molecular weights of proteins: A table, J. Chromatogr. 127, 1–28 (1976)
  21. Bassani G., Farruggia B., Nerli B., Romanini D. and Picó G., Porcine pancreatic lipase partition in potassium phosphate–polyethylene glycol aqueous two-phase systems, J. Chromatogr. B, 859, 222–228 (2007)
  22. Saravanan S., Raghava Rao J., Murugesan T., Balachandran Unni Nair and Ramasami T., Partition of tannery wastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH, Chem Eng Sci, 62, 969 – 978 (2007)