International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Hypolipidaemic effect of Leucodelphinidin derivative from Ficus bengalensis Linn on Cholesterol fed rats

Author Affiliations

  • 1Department of Medical Biochemistry, Faculty of Medicine, University of Gondar, ETHIOPIA
  • 2 Department of Biotechnology, Faculty of Natural and Computational Sciences, University of Gondar, ETHIOPIA
  • 3 Victoria Hospital, Bute Medical School, University of St Andrews, Scotland, UNITED KINGDOM
  • 4 Department of Biochemistry, University of Kerala, Trivandrum, INDIA

Res.J.chem.sci., Volume 2, Issue (2), Pages 54-60, February,18 (2012)


Administration of leucodelphinidin derivative isolated from the bark of Ficus bengalensis and another flavonoid quercetin (100mg/kg/day) in hypercholesterolemic rats provoked significant reduction in serum total cholesterol, LDL-cholesterol and an increase in the HDL-cholesterol levels. Significant decrease in atherogenic index was noted in these rats treated with the different flavonoids . There was an increased concentration of total bile acids in the liver and also increase in the fecal excretion of bile acids and neutral sterols in the rats fed cholesterol containing diet as compared to those fed normal diet. Feeding the flavonoids further significantly increased the concentrations of hepatic bile acids and the fecal excretion of bile acids and neutral sterols as compared to the control cholesterol diet fed group. The activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase was not inhibited by the flavonoids, instead there was an increased cholesterogenesis as was evident by an increased incorporation of labeled acetate into both free and esterfied cholesterol on treatment with leucodelphinidin and quercetin. These results demonstrated that the flavonoids exerted their hypocholesterolemic effects by increasing fecal bile acids and cholesterol excretion. Acute toxicity studies with the leucodelphinidin derivative showed no toxic reactions up to a dose of 4g/kg dose level.


  1. Babalola O.O., Ojo O.E. and Oloyeda F.A., Hepatoprotective activity of aqueous extract of the leaves of Hyptis suaveolens (l) Poit on acetaminophen induced hepatotoxicity in rabbits. Research Journal of Chemical Sciences,1(7), 85-88 (2011)
  2. Mathew B.C. and Daniel R.S., Effect of isoflavones on cardiovascular health: low but not out either. J. Clin Biochem.,43, 129-130 (2008)
  3. Mumanocha N., Chandra S.K., Sharma V., Sangameswaran B. and Saluja M., Anti-rheumatic and antioxidant activity of extract of stem bark of Ficus bengalensis,Research Journal of Chemical Sciences, 1(2), 2-8 (2011)
  4. Geetha B.S., Mathew B.C. and Augusti K.T., Hypoglycemic effects of leucodelphidin derivative isolated from Ficus bengalensis (Linn), Indian J Physiol Pharmacol., 38(3), 220-222 (1994)
  5. Augusti K.T., Hypoglycemic action of bengalenoside: Aglucoside isolated from Ficus bengalensis Linn, in normal and alloxan diabetic rabbits, Indian J Physiol Pharmacol., 19, 218-220 (1975)
  6. Augusti K.T., Daniel R.S., Cherian S., Sheela C.G. and Sudhakaran Nair C.R., Effect of leucope-lergonidin derivative from Ficus bengalensis Linn on diabetic dogs. Indian J Med Res., 99, 82-86 (1994)
  7. Cherian S., Sheela C.G., Augusti K.T., Insulin sparing action of leucopelergonidin derivative isolated from Ficus bengalensis Linn, Indian J Expl Biol., 33, 608-611 (1995)
  8. Kumar R.V., Augusti K.T., Insulin sparing action of a leucocyanidin derivative isolated from ficus bengalensis Linn, Indian J Biochem Biophys., 31(1), 73-76 (1994)
  9. Daniel R.S., Mathew B.C., Devi K.S., Augusti K.T., Antioxidant effect of two flavonoids from the bark of Ficus bengalensis Linn in hyperlipidemic rats, Indian J. Exp Biol., 36, 902-906 (1998)
  10. Daniel R.S., Devi K.S., Augusti K.T. and Sudhakaran Nair C.R., Mechanism of action of antiatherogenic and related effects of Ficus bengalensis Linn. Flavonoids in experimental animals, Indian J Exp Biol.,41, 296-303 (2003)
  11. Augusti K.T., Anuradha S. and Prabha S.P. et al. Nutraceutical effects of garlic oil, its nonpolar fraction and a Ficus flavonoid as compared to vitamin E in CCl4 induced liver damage in rats, Indian J Exp Biol.,43(5), 437-444 (2005)
  12. Prema M.S. and Misra G.S., Chemical constituents of Ficus bengalensis, Indian J Chem., 15B, 762-763 (1977)
  13. Zlatkis A., Zak B. and Boyle A.J., A new method for the direct determination of serum cholesterol, J. Lab Clin Med.,41, 486-492 (1953)
  14. Fossati P. and Prencipe L., Serum triglycerides determined colorimetrically with enzyme that produces hydrogen peroxide, Clin Chem.,28, 2077-2080 (1982)
  15. Gidez L.I., Miller G.J., Burstein M., Slagle S. and Eder H.A., Seperation and quantitation of subclasess of human plasma high density lipoproteins by a simple precipitation procedure. J. Lipid Res.,23, 1206-1223 (1982)
  16. Menon V.P. and Kurup P.A., Effect of fiber rich polysaccharide from blackgram on cholesterol metabolism in rats fed normal and atherogenic diet. Biomedicine, 24, 248-253 (1976)
  17. activity in liver tissue, Clin Chem., 21, 1523-1525 (1975)
  18. Zimmerman M., Ethical guidelines for investigation of experimental pain in conscious animals, Pain., 16, 109-110 (1983)
  19. Shukla R., Anand K., Prabhu K.M. and Murthy P.S., Hypocholesterolemic effect of water extract of the bark of banyan tree Ficus bengalensis, Indian J Clin Biochem., 10, 14-18 (1995)
  20. Egert S., Bosy-Westphal A. and Seiberi J., Quercetin reduces systolic blood pressure and plasma oxidized low-density lipoprotein concentrations in overweight subjects with high-cardiovascular disease risk phenotype: a double blinded, placebo-controlled cross-over study, Br J Nutr.,102(7), 1065-1074 (2009)
  21. Qureshi A.A., Reis J.C., Qureshi N., Papasian C.J., Morrison D.C. and Schaefer D.M., -Tocotrienol and quercetin reduce serum levels of nitric oxide and lipid parameters in female chickens. Lipids in Health and Disease, 10, 39, (2011)
  22. Yang T.T. and Koo M.W., Chinese tea lowers cholesterol level through an increase in fecal lipid excretion, Life Sci., 66(5), 411-423 (2000)
  23. Belquith-Hadriche O., Bouaziz M., Jamussi K., El Feki A., Sayadi S. and Makni-Ayedi F., Lipid lowering and antioxidant effects of an ethyl acetate extract of fenugreek seeds on high-cholesterol-fed rats, J Agric Food Chem.,58(4), 2116-2122 (2010)
  24. Leopoldini M., Malaj N., Toscano M., Sindona G. and Russo N., On the inhibitor effects of bergamot juice flavonoids binding to the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) enzyme, J. Agric Food Chem.,58(19), 10768-10773 (2010)
  25. Ngamukote S., Makyen K., Thialawech T. and Adisakwattana S., Cholesterol-lowering activity of the major polyphenols in grape seed, Molecules., 16(6)5054-5061 (2011)
  26. Crocenzi F.A., Pellegrino J.M., Sanchez Pozzi E.J., Mottino A.D., Garay E.A., Roma M.G., Effect of silymarin on biliary bile salt secretion in the rat, Biochem Pharmacol., 59(8), 1015-1022 (2000)
  27. Visavadiya N.P. and Narasimhacharaya A.V., Hypocholesterolemic and antioxidant effects of glycyrrhiza glabra (Linn) in rats, Mol Nutr Food Res., 50(11), 1080-1086 (2006)
  28. Lin Y., Vermeer M.A. and Bos W. et al., Molecular structures of citrus flavonoids determine their effects on lipid metabolism in HepG2 cells by primarily suppressing apoB secretion., J Agric Food Chem.,59(9), 4496-4503 (2011)
  29. Chen X.W., Serag E.S., Sneed K.B., Zhou S.F., Herbal bioactivation, molecular targets and toxicity relevance, Chem. Biol. Interact., 192(3), 161-176 (2011)