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α-Glucosidase inhibition, antioxidant and cytotoxicity activities of semi-ethanolic extracts of Bridellia ferruginea benth. and Ceiba pentandra L. Gaerth from Benin

Author Affiliations

  • 1Laboratoire d'Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d'Abomey Calavi/Université d'Abomey Calavi, Cotonou, BENIN
  • 2Laboratoire de chimie organique, UMR INSERM 990, Faculté de Pharmacie rue Montalembert, Clermont- Ferrand, FRANCE
  • 3Herbier National, Département de Botanique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, BENIN

Res.J.chem.sci., Volume 2, Issue (12), Pages 31-36, December,18 (2012)

Abstract

The use of plant extracts in the treatment of human disease requires a definition of optimal conditions. This study objective is to evaluate the inhibition capacity of -glucosidase activity of Bridelia ferruginea benth bark and Ceiba pentandra L. Gaerthroot semi-ethanolicextracts compared to acarbose. Their antioxidant activity were also tested by three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH)radical scavenging, Ferric reducing antioxidant power (FRAP) and the Oxygen Radical Absorbance Capacity(ORAC). Results indicated that these extracts have antioxidant property and -Glucosidase inhibitory activity (IC50 = 1.4 ± 0.04 µg/ mL for B. ferruginea and 51 ± 0.7 µg / mL for C. pentandra) higher than the reference compound acarbose (IC50 = 726 ± 15 mg / mL). Bridelia ferruginea extract was the most active of the two. In vitro cytotoxicity evaluation of the extracts was done by fluorometric assay: Resazurin reduction test onhuman fibroblast primary culture have showed a very low toxicity. Bridelia ferruginea and Ceiba pentandra The semi-ethanolic extracts could therefore constitute a credible alternative to replace the expensive synthetic drugs in the treatment of diabetes.

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