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Development of A Reversed-Phase High Performance Liquid Chromatographic Method for Efficient Diastereomeric Separation and Quantification of Cypermethrin, Resmethrin and Permethrin

Author Affiliations

  • 1 Centre for Chemical Sciences and Technology, Institute of Science and Technology, JNT University, Kukatpally, Hyderabad, INDIA

Res.J.chem.sci., Volume 2, Issue (10), Pages 26-31, October,18 (2012)


An efficient and simple reversed phase high performance liquid chromatographic method (RP-HPLC) for diastereomeric separation and quantification of cypermethrin, resmethrin, and permethrin has been developed. Separation was performed on Phenomenex Luna C18, (4.6 x 150 mm, 5 µm; end capped column). Satisfactory separation of diastereomers was obtained for the three pyrethroids studied. Good reproducibility of retention time and peak area were achieved. Detection was performed with UV diode array detector (UV-DAD) at a wavelength of 220 nm. Most peaks were base-separated with Rs values ranged from 1.6 to 4.5 for most peaks. The optimum mobile phase was composed of a mixture of acetonitrile, methanol, and water with a mixing ratio of 1:3:1, respectively. The regression coefficients (R2) were 0.9991, 0.9951 and 0.9964 with relative standard deviations (RSD%) of 1.95, 2.89 and 1.87, for cypermethrin (CYP), resmethrin (RES) and permethrin (PER), respectively.


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