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Chemical characterization of the coagulating solutions of the powders of the seeds of Arachis hypogaea L., Cucumeropsis mannii Naud. and Moringa oleifera Lam.

Author Affiliations

  • 1Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo and Institute for Research in Exact and Natural Sciences, BP 2400, Brazzaville, Congo
  • 2Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo
  • 3Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo
  • 4Multidisciplinary Research Team in Food and Nutrition, Faculty of Sci. and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo and Institute for Research in Exact and Natural Sciences, BP 2400, Brazzaville, Congo
  • 5Dina – BioRes# - Chem www.dinabiores.com 10 rue Simone Henry 31200 Toulouse, France
  • 6Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo

Res.J.chem.sci., Volume 11, Issue (3), Pages 14-23, October,18 (2021)

Abstract

The aim of this study is to characterize the proteins in the coagulating solutions of the powders of the seeds of Arachis hypogaea L., Cucumeropsis mannii Naud. and Moringa oleifera Lam. Analyzes were performed using high performance size exclusion liquid chromatography (SE-HPLC) on a Superdex column in the range of 10kDa to 500kDa and on a Shodex column in the range of 204Da to 35000Da. Electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS PAGE) was also performed. The results obtained showed that the coagulating solutions of C. mannii and M. oleifera mainly contain small proteins, of which 75.4% and 94.4% have molecular masses of less than 10kDa. The coagulating solution of A. hypogaea, on the other hand mainly contains large proteins, of which 25.4% have molecular masses between 100kDa and 300kDa, 16.8% between 300kDa and 500kDa and 16.8% have molecular masses greater than 500kDa.

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