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Hepatoprotective activity of aqueous extract of the leaves of Hyptis suaveolens (l.) Poit on acetaminophen Induced hepatotoxicity in rabbits

Author Affiliations

  • 1Department of Biochemistry, Obafemi Awolowo University, Ile Ife, NIGERIA
  • 2Department of Medicine, Federal Medical Center, Owo, NIGERIA
  • 3Department of Botany, Obafemi Awolowo University, Ile Ife, NIGERIA

Res.J.chem.sci., Volume 1, Issue (7), Pages 85-88, October,18 (2011)

Abstract

The study was designed to evaluate the possible hepatoprotective activity of the pretreatment with aqueous extract of the leaves of Hyptis suaveolens on acetaminophen induced hepatotoxicity in rabbits. Albumin, total protein, total globulin, alanine transaminase aspertate transaminase and catalase in the blood plasma and liver were assayed as biochemical markers of hepatotoxicity and possible associated oxidative stress. Plasma and total liver proteins, albumin, globulin concentrations were determined using standard spectrophotometric methods also the activities of alanine transaminase, aspertate transaminase and catalase in the plasma and liver homogenates were determined by standard techniques. The maker enzymes show significant elevation in acetaminophen treated animals; these were significantly reduced toward an almost normal level in animals pretreated with aqueous extract of the leaves of Hyptis suaveolens. The reduction was observed for the concentrations of total protein and albumin. This showed that aqueous extract of the leaves of Hyptis suaveolens in addition to many of its numerous reported bioactivity probably possesses hepatoprotective potentials on acetaminaphen induced liver damage.

References

  1. Fun C.E. and Svendsen A. B., The essential oil of Hyptis suaveolens,Poit grown on Aruba Flav Fragr J., , 161-163 (1990)
  2. Singh H.B. and A.K., Handique, Antifungal activity of the essential oil of Hyptis suaveolens and its efficacy in biocontrol measures in combination with Trichoderma harzianum,, 683-687 (1997)
  3. Iwu M.M., Handbook of African medicinal plants CRC Press Inc., Boca Raton, USA (1993)
  4. Zollo P.H.A., Biyiti L., Tchoumbougnang F., Menut C., Lamaty G. and Bouchet P.H., Aromatic plants of tropical Central Africa, Part XXXII, Chemical composition and antifungal activity of thirteen essential oils from aromatic plants of Cameroon, Flav Fragr J., 13, 107-114 (1998)
  5. Malele R.S., Mutayabarwa C.K., Mwangi J.W., Thoithi, G.N., Lopez A.G., Lucini E.I. and Zygadlo J.A., Essential oil of Hyptis suaveolens (L.) Poit from Tanzania: composition and antifungal activity, Journal of Essential Oil Research,15, 438–440 (2003)
  6. Jain S.R., Jain P.R. and Jain M.R., Antibacterial evaluation of some indigenous volatile oils, Planta Med., 26, 196-199 (1974)
  7. Asekun O.T., Ekundayo O. and Adeniyi B.A., Antimicrobial activity of the essential oil of Hyptis suaveolens leaves, Fitoterapia, 70, 440–442 (1999)
  8. Akah P.A. and Nwambie A.I., Nigerian plants with anticonvulsant properties, Fitoterapia, 64, 42–44 (1993)
  9. Ahmed M. Scora R.W. and Ting I.P., Composition of leaf oil of Hyptis suaveolens (L.) Poit, J. Essent, Oil Res., , 571-575 (1994)
  10. Cannon D.C, Olitzky I. and Inkpen J.A Protein, In Clinical Chemistry and Techniques, 2nd Edition, Henry R.J, Cannon D.C and Winkleman W (eds). Harper and Row, New York, 407-421(1974)
  11. Pinnel A.E. and Northam B.E., New automated dyebinding method for serum albumin determination with bromocresol purple, Clin Chem., 24-80 (1978)
  12. Reitman S. and Frankel A., colorimetric method for aspertate and alanine aminotransferase in the serum, J. Clin Pathol, 28, 56-58(1957)
  13. Claiborne A., Catalase activity In: Greenwald R.A. (Ed.), Handbook of Methods for Oxygen Free Radical Research CRC Press, Boca Raton, FL (1985)
  14. Sies H. Strategies of antioxidant defense, Eur J Biochem. (215) 213 -219 (1993)
  15. Harper H.A., The functions and tests of the liver. Review of Physiological Chemistry Lange Medical Publishers, Los Atlos, CA, 271– 283 (1961)