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Validated HPTLC Method for the Determination of Tenofovir as Bulk Drug and in Pharmaceutical Dosage Form

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Author Affiliations

  • 1School of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan INDIA
  • 2 Shri Gopichand College of Pharmacy, Baghpat, Uttar Pradesh INDIA

Res.J.chem.sci., Volume 1, Issue (7), Pages 33-37, October,18 (2011)

Abstract

A simple, accurate, precise and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Tenofovir in tablet dosage forms. The method employed TLC aluminium plates precoated with silica gel 60 F 254 as the stationary phase. The mobile phase used was a mixture of (Chloroform: Methanol 8.5: 1.5v/v). The detection of spot was carried out at 270nm. The calibration curve was found to be linear between 200 to 1200 ng mL-1 with regression coefficient of 0.9994. The proposed method can be successfully used to determine the drug content of marketed formulation. The accuracy of the proposed method was determined by recovery studies and found to be 98.39 to 101.19 %. The proposed method is applicable to routine analysis of Tenofovir in bulk and pharmaceutical formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection, limits of quantification, range and solution stability.

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