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Development and Validation of New RP-HPLC Method for the Determination of Cefaclor in Pharmaceutical Dosage forms and in Human Plasma

Author Affiliations

  • 1Department of Chemistry, Krishna University, Machilipatnam
  • 2 Department of Chemistry, NIMS University, Rajasthan, India

Res.J.chem.sci., Volume 1, Issue (5), Pages 36-39, August,18 (2011)


A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of Cefaclor in pharmaceutical dosage forms and in serum. Chromatography was performed by Shimadzu model LC-20 ATVP with Kromasil C-18 column using a mobile phase comprising of acetonitrile: orthophosphoric acid (1%) : 0.01M ammonium dihydrogen phosphate (50:45:5 v/v), at a flow rate 1.0ml/min. The analyte was monitored using PDA detector at 270nm and the Run time was 10min. for Cefaclor. The proposed method was found to have linearity in the concentration range of 2-10 µg/ml.


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