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Effect of digoxin treated mulberry leaves on Protein profiles in fifth instar larvae of Silkworm, Bombyx mori (L) (PM x CSR2)

Author Affiliations

  • 1Dept. of Zoology, Shardabai Pawar Mahila College, Shardanagar, Tal-Baramati,Pune- 413115, INDIA
  • 2Dept. of Zoology, Vidya Pratishthan College, Baramati. Pune – 413102, INDIA.

Res.J.chem.sci., Volume 1, Issue (1), Pages 2-7, April,18 (2011)

Abstract

The tablets of digoxin (Lanoxin) were to dissolved in water to prepare ten part per million (ten mg per litre) solution. The mulberry leaves were soaked in the stock solution of digoxin for half an hour. The Digoxin treated leaves were drained and fed to the fifth instar larvae of multivoltine cross breed (PM x CSR2) for the second, third, fourth and fifth day (from 48 to 120 hours after the forth moult ). The Larvae fed with untreated and water treated mulberry leaves were also maintained. Biochemical estimation of proteins (Soluble and total) was carried out at 120 hours after the fourth moult. Feeding the larvae with digoxin treated mulberry leaves were found variously reflected into improvement in protein profile of whole body, haemolymph and silk glands. Pattern of increase in soluble proteins and total proteins in whole body and haemolymph was found similar (36.584; 47.87; and 87.963, 91.428 percent respectively). Increased level of tissue proteins of silk glands was found 54.776 (soluble) and 43.373 (Total) percent. Improvement of protein profiles in the larvae fed with digoxin treated mulberry leaves may be explained away as due accelerated rate of digestion, absorption in the alimentary canal. Digoxin titre in the larval body may influence the development of tissue especially the silk glands that causes to accelerate the protein accumulation. Digoxins, the glycoside, exert acceleratory influence, especially on the midgut- glucosidase activity. Digitoxose, the glycone moiety of digoxin deserve cardiotonic activity. It may be improving cardiac physiology in silk worm. Feasible method for using digoxin for rearing larvae of silk worm should be established.

References

  1. Agarell I.P.S. and Lundguist A.M., Physiology and biochemical changes during insect development in: The physiology of insects, Academic press New York, (2) 1,(1971)
  2. Riddiford L.M., Hormone action at the cellular level. In: Comprehensive Insect Physiology Biochemistry and Pharmacology Eds. G. A. Kerkut and L.I. Gilbert, Pergamon Press Oxford, 8, 37-84 (1985)
  3. Riddiford L.M., Cellular and molecular actions of juvenile hormone: General consideration and premetamorphic actions, J. Adv. Insect physiology, 24, 213 – 214 (1994)
  4. Sehnal F. and Rambold, Brain stimulation of juvenile hormone production in insect larvae, Experientia, 41, 684 – 685 (1985)
  5. Bowers W.S., Fales V.M., Thomson M.J. and Uebel B., Juvenile and gonadotropic activity of 10 11 – epoxyfarnesoic acid methyl ester, Life Science, 4, 2323 – 2331 (1966)
  6. Slama K., Plant as a source material with insect hormone activity, Ent. Exp. Appl., 12, 721-728 (1969)
  7. Karlson P. and Schmialek P., Nachweis der Exkretion von juvenilhormonen Z. Naturf. 14 b: 821 (1959)
  8. Manville J. F., Gregus L., Slama K. and Von Rudoloff E., Occurrence of Juvabione type/ epijuvabione type insects juvenile hormone analogues in three Czechoslovakian firs., Coll. Czech. Chem. Commun., 42, 3658- 3666 (1977)
  9. Krishnaswami S., Narasimhana M. N., Suryanarayana S. K. and Kumararaj S., Sericulture Manual- II: Silkworm Rearing, FAO United Nations Rome, 131 1973) and (1978)
  10. Lowery O.H., Rosenbrough N.J., Far A.L. and Randall R.J., Protein measurement with folin phenol reagent., J. Biol. Chem.,193, 265-275 (1951)
  11. Norman T.J. and Baily, Statistical methods in Biology (1955)
  12. Benchamin K.V., Jolly M.S., Benjamin D.A.I. and Ratnasen, The commercial application of juvenile hormone analogue ‘Manta ‘in silkworm Bombyx mori (L) and the economics, In: National seminar on silk research and Development, Central Silk Board Banglore, Page–73 (1983)
  13. Subba Rao G., Das S. K., Nandi S., Sompou R. and Sen S.K., Sericologia,28 (a), 543-548 (1988)
  14. Rajashekhargouda R., Studies on methods to increase the silk yield of Bombyx mori (L), Ph. D. Thesis, Tamil Nadu Agricultural University, 243, (1991)
  15. Mamatha D.N., Nagalakshamma K. and Rajeshwara Rao M., Impact of selected Juvenile Hormone mimics on the organic constituents of silkworm Bombyx mori (L), Proceedings of NSTS – 99, 185 – 186 (1999)
  16. Vitthalrao B. Khyade, Patil S. B., Khyade S. V., and Bhawane G. P., Influence of acetone maceratives of Vitis vinifera on the larval parameters of silkworm Bombyx mori (L)., IndianJournal of Comparative Animal Physiology, 20, 14-18 (2002)
  17. Vitthalrao B. Khyade, Patil S.B., Khyade S.V. and Bhawane G.P., Influence of acetone maceratives Vitis vinifera on the economic parameters of silkworm Bombyx mori (L), Indian Journal of Comparative Animal Physiology, 21, 28-32, (2003)
  18. Vitthalrao B. Khyade, Influence of Juvenoids on silkworm Bombyx mori (L), Ph. D.Thesis, Shivaji University Kolhapur India (2004)
  19. Vitthalrao B. Khyade and Ganga V. Mhamane,, Vividh Vanaspati Arkancha Tuti Reshim Kitak Sangopanasathi Upyojana, Krishi Vidnyan, 18-22 (2005)
  20. Vitthalrao B. Khyade and Jiwan P. Sarwade, Protein profiles in the fifth instar larvae of silkworm Bombyx mori (L), The Bioscan, 4(1), 41-44 (2009)
  21. Ghantaloo U.S., Influence of Digoxin on silkworm Bombyx mori (L), M.Phil Thesis, Alagappa University Karaikudi (Tamil Nadu) India (2007)
  22. Jagtap S.G., Effect of plant juvenoids on consumption and utilization of mulburrey leaves by silkworm Bombyx mori (L) M.Phil Thesis, Algappa University Karaikudi, Tamil Nadu (India) (2007)
  23. Shigematsu H., The nature of juvenoid on growth and Changes of protein amino acid and nucleic acid content in silkworm Bombyx mori (L) in the case of application of excessive dose, J. seric. Japan, 47, 292-300 (1978)
  24. Sen S. K., Sericologia, 28 (a), 543–548 (1988)
  25. Doane W.W., Role of hormone in insect’s developmental systems: Insects Ed. S.J., Counce Wadingaton, London/ New York: Academic Press, 1, 291 – 497. (1973)
  26. Cerny V., Doles L., Labler L., Sorm F. and Slama K., Dehydrojuvobione: a new compound with juvenile hormone activity from balsam fir, Tetrahedron Lett., 12, 1053 – 1057 (1967)
  27. Vinod D. Rangari, Glycosides. In: Pharmacognasy and Phytochemistry, First edition, Career Publication Nashik, 238-241 (2009)
  28. Williams C.M., The Juvenile Hormone of Insects, Nature,178, 212- 213 (1956)
  29. Williams C.M., The Juvenile Hormone- I Endocrine activity and corpora allata of the adult Cecropia silkworm, Biol. BullWoods Hole, 116, 323 – 338 (1959)
  30. Zaoral M. and Slama K., Peptides with juvenile hormone activity, Science, 170, 92-93 (1970)