6th International Virtual Congress (IVC-2019) And Workshop.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Prevalence of ESBL And AmpC β-Lactamase in Gram Negative Bacilli in various Clinical Samples at Tertiary Care Hospital

Author Affiliations

  • 1 Department of Microbiology, NIMS Medical College, NIMS University, Jaipur, Rajasthan, INDIA

Int. Res. J. Medical Sci., Volume 3, Issue (8), Pages 1-6, August,28 (2015)

Abstract

The rapid dispersal of antibiotic resistance as extended spectrum beta lactamases (ESBLs) and AmpC β-lactamases in bacteria makes a major public health concern worldwide. It becomes essential to guide the clinicians about the Knowledge of their occurrence and the appropriate anti-microbial treatment. This study was done to evaluate the Prevalence of ESBL and AmpC β-lactamases and their antibiotic susceptibility in gram-negative clinical isolates were analyzed. Total 200 non repetitive clinical isolates of {Escherichia coli (n=101), Klebsiella spp (n=41), Citrobacter spp. (n=22), Pseudomonas spp. (n=20), Proteus spp.(n=5), Acinetobacter spp.(n=7) and Enterobacter spp.( n=4)} obtained over a period from (January to August, 2014), were screened by Kirby Bauer disc diffusion method for ESBLs and AmpC production and suspected isolates were confirmed by combined disc and AmpC disc tests. From 117(58.5%) and 62 (31%) screened out isolates, 87(43.5%) and 44(22%) were found to be ESBL and AmpC producers respectively. The distribution of ESBL and AmpC isolates organism wise showed E.coli (44.5% and 21.9%), Klebsiella spp (41.4% and 24.3%), Pseudomonas spp. (30% and 20%) and Acinetobacter spp (28.6 and 14.3%) respectively and they were found significantly multidrug resistance too. The co-existence phenotype of both ESBLs and AmpC were 23(11.5%) isolates. In our hospital moderate prevalence of ESBLs and AmpC was found. Combination disc test was effective for ESBL detection While the AmpC disc test, was found to be a convenient, specific and highly sensitive. Regular monitoring is necessary for the incidence of the ESBL and AmpC β-lactamase production by organisms.

References

  1. Chaudhary U and Aggarwal R. Extended Spectrum β-lactamases: An emerging threat to clinical therauptics, IJMM, 22(2), 75-80 (2004)
  2. Betty A Forbes, Daniel F and Alice S Weissfeld, In: Diagnostic microbiology,13th Ed.: Andrew allen Publisher; 162,163, 202-229 (2013)
  3. CLSI, Performance Standards for Antimicrobial Susceptibility Testing, Twentieth Informational Supplement, CLSI Document M100-S20, Wayne, PA: Clinical and Laboratory Standards Institute, CLSI (2010)
  4. Laghawawe AH, Jaitly and N Thombare, The Simultaneous Detection of the ESBL and the Amp C β-lactamases in Gram Negative Bacilli, Journal of Clinical and Diagnostic Research, 2, 6(4) 660-663 (2012)
  5. Thomson KS, Controversies about Extended-Spectrum and Amp C β-lactamases, Emerging Infectious Diseases, 7(2), (2001)
  6. Shanthi M, Sekar U and Arunagiri K, Detection of Amp C genes encoding for β-lactamases in E.coli and Klebsiella pneumonia, IJMM, 30(3), 290-5 (2012)
  7. Getzlaff SP, Polsfuss S and Poledica M et al., Detection of AmpC β-lactamases in Escherichia coli: Comparison of Three Phenotypic Confirmation Assays and Genetic Analysis, Journal of clinical Microbiology, 49(8), 29242932 (2011)
  8. Taneja N, Singh G and Singh M et al., High occurrence of blaCMY-1 AmpC lactamase producing Escherichia coli in cases of complicated urinary tract infection (UTI) from a tertiary health care centre in north India, Indian J Med Res, 136, 289-291 (2012)
  9. Khan MKR, Thukral SS and Gaind R, Evaluation of a modified double-disc Synergy test for detection of extended spectrum of β-lactamases in AmpC β-lactamase producing Proteus Mirabilis, IJMM, 26(1), 58-61 (2008)
  10. Tsering DC, Das S and Adhiakari L et al., ESBL Detection in Gram-negative Bacilli of Nosocomial Origin, J Glob Infect Dis., 1(2), 8792 (2009)
  11. Laghawe AR, Jaitly NK and Thombare V., Prevalence of Amp C Beta-lactamase in Gram- negative bacilli, Journal of pharmaceutical and biomedical sciences, 20(07) (2012)
  12. Black JA, Moland ES and Thomson KS, Amp C Disc Test for Detection of Plasmid-Mediated Amp C β-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC β -Lactamases, Journal of clinical microbiology, 43(7), 31103113 (2005)
  13. Konemann EW, Winn WC, Jr, Allen SD, Janda WM, ,Procorp GW, Schreckenberger PC and Woods GL, Konemanns color atlas and textbook of diagnostic Microbiolgy, 6th edition, Philadelphia: LIPINCOTT WILLIAMS and WILKINS, 1003, 21-35, 217-218,1443-1457 (2006)
  14. Singh RKM, Pal NK, Banerjee M, Sarkar S and Sen Gupta M, Surveillance on Extended Spectrum β-lactamase and AmpC β-lactamase producing gram negative isolates from nosocomial infections, Archives of clinical microbiology, 3(3), 1 (2012)
  15. Chandel DS, Johnson JA and Chaudhry R et al., ESBL-producing Gram-negative bacteria causing neonatal sepsis in India in rural and urban settings, Journal of Medical Microbiology, (60), 500507 (2011)
  16. Wadekar MD, Anuradha K and Venkatesha D, Phenotypic detection of ESBL and MBL in clinical isolates of Enterobacteriaceae, International journal of current research and academic review, 1(3), 89-95 (2013)
  17. Hoşoğlu S, Gndeş S and Kolayl F et al., ESBLs in ceftazidime resistant E.coli and Klebsiella Pneumoniae isolates in Turkish Hospitals, IJMM, 25(4), 346-5 (2007)
  18. Sharma M, Pathak S and Srivastava P, Prevalence and antibiogram of ESBL producing Gram negative bacilli and further molecular characterization of ESBL producing Escherichia coli and Klebsiella spp., Journal of Clinical and Diagnostic Research, 7(10), 2173-2177 (2013)
  19. Rudresh S.M. and Nagarathnamma T., Two simple modifications of modified three-dimensional extract test for detection of AmpC β-lactamases among the members of family Enterobacteriaceae, Chronicles of Young Scientists, 2(1), 42-6 (2011)
  20. Polsfuss S, Bloemberg GV and Giger J et al., Practical Approach for Reliable Detection of AmpC Beta-Lactamase-Producing Enterobacteriaceae, Journal of clinical microbiology, 49(8), 27982803 (2011)
  21. Arora S and Bal M., AmpC β-lactamase producing bacterial isolates from Kolkata hospital, Indian J Med Res, 122, 224-233 (2005)
  22. Bandekar N, Kumar VCS and Basavarajappa K.G. et al., Beta lactamases mediated resistance amongst gram negative bacilli in Burn infection, Int J Biological and Medical Resarch, 2(3), 766-770 (2011)
  23. Sasirekha B., Prevalence of ESBL, AMPC β-Lactamases And MRSA Among Uropathogens and its Antibiogram, EXCLI Journal, 12, 81-88 (2013)
  24. Sinha P, Sharma R and Rishi S et al., Prevalence of ESBL and AmpC β-lactamase producers among Escherichia coli isolates in a tertiary care hospital in Jaipur, IJPM., 51(3), (2008)
  25. Nayar R, Arora VM and Duggal S., Antibiotic impregnated tablets for screening ESBL and AmpC beta lactamases, Journal of Pharmacy, 2(2), 207-209 (2012)
  26. Dalela G, Gupta S, Jain DK and Mehta P, Antibiotic Resistance Pattern in Uropathogens at a Tertiary Care Hospital at Jhalawar with Special Reference To Esbl, AmpC b-Lactamase and Mrsa Production, Journal of clinical and Diagnostic research, 6(4), 645651 (2012)
  27. Gupta V, Rani H and Singla N et al., Determination of ESBL and AmpC Production in Uropathogenic Isolates of Escherichia coli and Susceptibility to Fosfomycin, Journal of Laboratory Physicians , 5(2), 90-93 (2013)
  28. Jain A and Mondal R., Prevalence and antimicrobial resistance pattern of ESBL producing Klebsiella spp isolated from cases of neonatal septicaemia, Indian J Med Res, 125, 89-94 (2010)
  29. Kothari C, Gaind R and Singh LC et al., Community acquisition of β-lactamase producing Enterobacteriaceae in neonatal gut, BMC Microbiology, 13, 136 (2013)