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Chitinase production from Seafood wastes by Plant pathogen Bionectria CBNR BKRR sps and its application in Bioremediation studies

Author Affiliations

  • 1 Department of Biotechnology, Maharaja Co-education Arts and Science College, Perundurai, Erode, INDIA
  • 2 Synkromax Biotech Private Limited, Chennai, INDIA

Int. Res. J. Medical Sci., Volume 2, Issue (11), Pages 15-19, November,28 (2014)

Abstract

Bionectria CBNR BKRR, isolated from the marine soils of Pichavaram, Tamil Nadu was used successfully for the biodegradation of four different sea food wastes-Crab shell, Snail shell, Shrimp shell, Fish scales to produce highly active chitinase enzyme. The crude chitinase so extracted was characterized and maximum activity was calculated from the reaction mixture containing, 2 ml crude enzyme, 0.5 ml of 10% colloidal chitin, pH 6 and a reaction time of 10 min at 50°C incubation temperature. Maximum chitinase enzyme activity was obtained in the case of fish scales (5.8 U/min) Shrimp wastes (4.0 U/min), followed by Snail shell (3.9 U/min), Crab shell (3.7 U/min). The amount of seafood wastes degraded was as follows-Crab shell (0.27g), Snail shell (0.01g), Shrimp shell (0.42g) and Fish scales (1.64g).The results indicated that Bionectria CBNR BKRR is efficient fungus to produce highly active chitinase when grown in statistically optimized medium containing Snail shell, Crab shell, Fish scales and Shrimp shells as substrates.

References

  1. Bansode VB and Bajekal SS, Characterization of chitinases from microorganisms isolated from Lonar Lake, Indian J. Biotechnol., 364-367 (2006)
  2. Ravi Kumar MNV, A review of chitin and chitosan applications, Reactive and Functional Polymers., 46, 1-27 (2000)
  3. Mejia-Saules JM, Waliszewski KN, Garcia MA and Cruz-Camarillo R, The use of crude shrimp shell powder for chitinase production by Serratia marcescens WF, Food Technol. Biotechnol., 44(7), 646-651 (2006)
  4. Kuk JH, Jung WJ, Jo GH, Kim YC and Park RD, Production of N-acetyl-D-glucoseamine from chitin by Aeromonas sp. GJ-18 crude enzyme, Appl. Microb. Biotechnol., 68, 384-389 (2005)
  5. Dahiya N, Tewari R and Hoondal G., Biotechnological aspects of chitinolytic enzymes : A review, Appl. Microbiol. Biotechnol. , 71, 773-782 (2005)
  6. Guevara-Gonzalez RG and Torres-Pacheco, I Advances in Agricultural and Food Biotechnology,Kerala, India, Research Signpost(2006)
  7. Chae D, De Jin R, Hwangbo H, Kim Y, Park R, Krishnan H and Kim K, Control of late blight (Phytophthoracapsici) in pepperplant with a compost containing multitude of chitinase-producing bacteria, Biocontrol, 51, 339-351 (2006)
  8. Wang SL and Hwang JR., Microbial reclamation of shellfish waste for the production of chitinase, J. Enzyme Microbiol. Technol., 28, 376-382 (2001)
  9. Faramarzi MA, Fazeli M, Yazdi MT, Adrangi S, Al-Ahmadi KJ, Tasharrofi N and Mohseni FA., Optimization of cultural conditions for production of chitinase by a soil isolate, Massilia timonae. Biotechnol Bioseparation, 8(1), 93-99 (2009)
  10. Pedraza RM and Lopez RE., Chitinase activity in germinating cells of Mucor rouxii Antonie van Leeuwenhoek, 59, 183- 189 (1991)
  11. Wang SL and Chio SH., Deproteinization of shrimp and crab shell with the protease of Pseudomonas aeruginosa K-187, Enzyme Microb. Technol., 22, 629-633 (1998)
  12. Rattanakit N, Yang S, Wakayama M, Plikomol A and Tachiki T., Saccharification of chitin using solid-state culture of Aspergillus sp. S1-13 with shellfish waste as a substrate, J. Biosci. Bioeng., 95,391-396 (2003)
  13. Binod P, Pusztahelyi T, Nagy V, Sandhya C, Szakács G, Pócsi I and Pandey A, Production and purification of extracellular chitinases from PenicilliumaculeatumNRRL 2129 under solid-state fermentation, Enzy. Microbial Technol., 36, 880-887 (2005)
  14. Collier L., Balows A and M. Sussman., Topley and Wilson's, Microbiology and Microbial Infections, Arnold, London, Sydney, Auckland, New York, 9(4),(1998)
  15. St-Germain G and R Summerbell, Identifying Filamentous Fungi - A Clinical Laboratory Handbook, Star Publishing Company, Belmont, California, 1,(1996)
  16. Sparling GP, Fermor TR and Wood DA., Measurement of the microbial biomass in composted wheat straw, and the possible contribution of the biomass to the nutrition of Agaricus bisporus,Soil Biol Biochem, 14, 609-611 (1982)
  17. Wiegant WM., A simple method to estimate the biomass of thermophilic fungi in composts, Biotechnology Techniques, 421- 426 (1992)
  18. Miller FC. Composting of Municipal Solid Waste and its Components. In : Palmisano AC, Barlaz MA, eds. Microbiology of Solid Waste. CRS Press. p 115-154 (1996)
  19. Haran S, Schickler H and Chet I., Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum, Microbiology,142, 2321-2331 (1996)
  20. Sharaf EF., A potent chitinolytic activity of Alternariaalternata isolated from Egyptian black sand, Polis J. Microbiol., 54, 145-151 (2005)
  21. Sandhya C, Adapa LK, Nampoothiri KM, Binod P, Szakacs G and Pandey A., Extracellular chitinase production by Trichoderma harzianum in submerged fermentation. J. Basic Microbiol., 44, 49-58 (2004)
  22. Ghanem KM, Al-Garni SM and Al-Makishah NH., Statistical optimization of cultural conditions for chitinase production from fish scales waste by Aspergillus terreus. Afr. J. Biotechnol., 9(32), 5135-5146 (2010)
  23. Ghanem KM, Fahad A. Al-Fassin and Reem M. Farsi., Statistical optimization of cultural conditions for chitinase production from shrimp shellfish waste by Alternaria alternata Afr. J. Biotechnol., 1649-1659 (2011)
  24. Rattanakit N, Yano S, Plikomol A, Wakayama M, and Tachiki T., Purification of Aspergillus sp. S1-13 chitinases and their role in saccharification of chitin in mash of solid-state culture with shellfish waste, J. Biosci. Bioeng., 103, 535-541 (2007)
  25. AL-Nusaire TS., Degradation of some marine polymers by microorganisms isolated from Al-Jouf region, M.Sc. Thesis, Girl S College of Education. King Abdulaziz University, Jeddah, Saudi Arabia, (2007)
  26. Krishnaveni B. and Ragunathan R., Chitinase production from marine wastes by Aspergillus terreus and its application in degradation studies. Int. J. Curr. Microbiol. App. Sci, 3(1), 76-82 (2014)
  27. http://www.ncbi.nlm.nih.gov/BLAST www.ebi.ac. uk/ clustalw), (2014)