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Chitinase production from Seafood wastes by Plant pathogen Bionectria CBNR BKRR sps and its application in Bioremediation studies

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Author Affiliations

  • 1 Department of Biotechnology, Maharaja Co-education Arts and Science College, Perundurai, Erode, INDIA
  • 2 Synkromax Biotech Private Limited, Chennai, INDIA

Int. Res. J. Medical Sci., Volume 2, Issue (11), Pages 15-19, November,28 (2014)

Abstract

Bionectria CBNR BKRR, isolated from the marine soils of Pichavaram, Tamil Nadu was used successfully for the biodegradation of four different sea food wastes-Crab shell, Snail shell, Shrimp shell, Fish scales to produce highly active chitinase enzyme. The crude chitinase so extracted was characterized and maximum activity was calculated from the reaction mixture containing, 2 ml crude enzyme, 0.5 ml of 10% colloidal chitin, pH 6 and a reaction time of 10 min at 50°C incubation temperature. Maximum chitinase enzyme activity was obtained in the case of fish scales (5.8 U/min) Shrimp wastes (4.0 U/min), followed by Snail shell (3.9 U/min), Crab shell (3.7 U/min). The amount of seafood wastes degraded was as follows-Crab shell (0.27g), Snail shell (0.01g), Shrimp shell (0.42g) and Fish scales (1.64g).The results indicated that Bionectria CBNR BKRR is efficient fungus to produce highly active chitinase when grown in statistically optimized medium containing Snail shell, Crab shell, Fish scales and Shrimp shells as substrates.

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