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Effect of Explants and Plant Growth Regulators on Callus Induction in Ricinus communis L

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Author Affiliations

  • 1Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, SUDAN
  • 2 Commission of Biotechnology and Genetic Engineering, National Centre for Research, P. O.Box 2404, Khartoum, SUDAN

Res. J. of Pharmaceutical Sci., Volume 4, Issue (1), Pages 1-6, March,30 (2015)

Abstract

The present research work aimed to shedding light on the effect of various plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and -Naphthalene Acetic Acid (NAA) on callus induction of ricinus commun. Callus culture were initiated from cotyledonary leaf and root segments explants from in vitro Ricinus communis L seedling . After four day of inoculation callusing was observed from cotyledonary leaf segment explants, after about one week from root segment explant. Auxins 2,4-D and NAA effecting in callus agitationbut 2.5 mg/L 2,4-D proved to be more effected for induction of callus in R. communis L. Individual treatment of NAA reveal low effectiveness for callus induction. Among all treatment media comprising 2,4 D (2.5 mg/l) showed superior response of callus degree (4 .0±0.0) with cotyledonary leaf segment explant and at this concentration, maximum amount of callus was observed whereas, root segment explants showed (2.7±0.19 ) degree of callus response in the same concentration . The callus from cotyledonary leaf segment explants was Light brown colour with compact nature in media fortified with 2,4 D while in media fortified with NAA callus was Light green colour with Friable nature. The callus from root segment explants was creamy to yellow colour with compact nature with the both tested auxin.

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