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Production, Characterization and Analysis of Melanin from Isolated Marine Pseudomonas sp. using Vegetable waste

Author Affiliations

  • 1Department of Chemical Engineering, National Institute of Technology, Rourkela, 769008, Orissa, INDIA

Res. J. Engineering Sci., Volume 2, Issue (5), Pages 40-46, May,26 (2013)


Melanin pigment from natural sources like microorganisms was an attractive choice for commercial scale production. In this study, marine bacterium capable of melanin production on marine broth/agar was isolated and identified as Pseudomonas sp. (closely related to guinea) on phenotypic characterization. Melanin production activity of the isolate was studied in liquid mediums such as pure marine broth and vegetable cabbage waste. In pure marine broth, melanin yield was ~5.35 mg/mL and pigment production was absent in pure vegetable waste. However in the presence of marine broth (as starter culture) melanin yield increased to ~2.79 mg/mL. This indicates melanin production may be initiated austerely by marine broth. Pigment from the bacterium was purified and characterized using UV-visible and FTIR analysis. The morphology and size of the bacterium was visualized in scanning electron microscopy (SEM) and the pigment nature was identified by SEM/EDX analysis. The results indicated that the synthesized melanin was very near to synthetic dihydroxyphenylalanine (DOPA)-melanin in all aspects and possess antioxidant activity.


  1. Sajjan S., Purification and physiochemical characterization of melanin pigment from Klebsiella sp. GSK, J. Microbiol.Biotechnol., 20, 1513–1520 (2010)
  2. Geng J., Yuan P., Shao C., Yu S. B., Zhou B., Zhou P., and Chen X. D., Bacterial melanin interacts with doublestranded DNA with high affinity and may inhibit cell metabolism in vivo, Arch. Microbiol., 192, 321–329 (2010)
  3. Gómez-Marín A. M., and Sánchez C. I., Thermal and mass spectroscopic characterization of a sulphur-containing bacterial melanin from Bacillus subtilis, J. Non-Cryst. Solids., 356, 1576–1580 (2010)
  4. Harki E., Talou T., and Dargent R., Purification,characterisation and analysis of melanin extracted from Tuber melanosporum Vitt, Food Chem., 58, 69–73 (1997)
  5. Coyne V. E., and Al-Harthi L., Induction of melanin biosynthesis in Vibrio cholera, Appl. Environ Microbiol., 58, 2861–2865 (1992)
  6. Youngchim S., Morris-Jones R., Hay R. J., and Hamilton A.J., Production of melanin by Aspergillus fumigatus, J. Med.Microbiol., 53, 175–181 (2004)
  7. Kotob S. I., Coon S. L., Quintero E. J., and Weiner E. M.,Homogentisic acid is the primary precursor of melanin synthesis in Vibrio cholerae, a Hyphomonas strain, and Shewanella colwelliana, Appl. Environ. Microbiol., 61, 1620–1622 (1995)
  8. Solano F., Garcia E., Perez D., and Sanchez-Amat A., Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium, Appl. Environ. Microbiol., 63, 3499–3506 (1997)
  9. Lucas-Elío P., Goodwin L., Woyke T., Pitluck S., Nolan M., Kyrpides N. C., Detter J. C., Copeland A., Teshima H., Bruce D., Detter C., Tapia R., Han S., Land M. L., Ivanova N., Mikhailova N., Johnston A. W. B., and Sanchez-Amat A., Complete genome sequence of the melanogenic marine bacterium Marinomonas mediterranea type strain (MMB-1T), Stand. Genomic. Sci., 6, 63–73 (2012)
  10. Quadri S. R., and Agsar D., Detection of melanin producing thermo-alkaliphilic Streptomyces from limestone quarries of the Deccan traps, World J. Sci. Technol., 2, 8-12 (2012)
  11. Ravishankar J. P., Muruganandam V., and Suryanarayanan T. S., Isolation and characterization of melanin from a marine fungus, Bot. Mar., 38, 1–6 (1995)
  12. Ju K. Y., Lee Y., Lee S., Park S. B., and Lee J. K., Bioinspired polymerization of dopamine to generate melanin like nanoparticles having an excellent free radical scavenging property, Biomacromolecules 12, 625–632 (2011)
  13. van de Sande W. W., de Kat J., Coppens J., a Ahmed A. O., Fahal A., Verbrugh H., and van Belkum A., Melanin biosynthesis in Madurella mycetomatis and its effect on susceptibility to itraconazole and ketoconazole, Microbes Infect., 9, 1114–1123 (2007)
  14. Coates J., Interpretation of infrared spectra, a practical approach, Wiley Online Library (2000)