6th International Virtual Congress (IVC-2019) And Workshop.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Assessment of polyethylene degradation by Aspergillus niger using submerged cultivation and soil burial method

Author Affiliations

  • 1Department of Environmental Science, Shivaji University, Kolhapur, MS, India
  • 2Department of Environmental Science, Shivaji University, Kolhapur, MS, India
  • 3Department of Environmental Science, Shivaji University, Kolhapur, MS, India

Int. Res. J. Environment Sci., Volume 7, Issue (7), Pages 16-22, July,22 (2018)

Abstract

Polyethylene (PE) has occupied a vital role in human life but the real problem has emerged due to post-use of plastic. Waste plastic lays enormous burden on the environment, because their recalcitrance to degradation accelerates its accumulation in nature. In the present study, the feasibility of a fungus, Aspergillus niger for active degradation of Low Density Polyethylene (LDPE) and High Density Polyethylene (HDPE) was examined by submerged cultivation and soil burial method. Test flasks were subjected for incubation on a rotary shaker at laboratory condition for 30 days where PE was only carbon source. PE degradation was confirmed by 3.97% weight loss in submerged cultivation method and 1.89% in soil burial method. Major change in functional group or formation of side chain was not observed on FTIR Spectroscopy analysis but the reduction in percentage transmittance after 60 days on incubation with fungus Aspergillus niger reveals the microbial activity on PE and thus confirms the degradation of PE. Hence, Aspergillus niger proves more efficient in submerged cultivation method rather than soil burial method.

References

  1. Report (2001)., Statistics of Foreign Trade of India., DGFT, GoI.
  2. Ghosh S.K., Pal S. and Ray S. (2013)., Study of microbes having potentiality for biodegradation of plastics., Environmental Science and Pollution Research, 20, 4339-4355.
  3. Kumari K., Aanad R.C. and Narula N. (2009)., Microbial degradation of Polyethylene (PE)., The South Pacific Journal of Natural Science, 27, 66-70.
  4. Hadad D., Geresh S. and Sivan A. (2005)., Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis., Appl. Microbio., 98(5), 1093-1100.
  5. Shah A.A., Hasan F., Hameed A. and Akhter J. (2009)., Isolation of Fusarium sp AF4 from sewage sludge, with the ability to adhere the surface of polyethylene., African Journal of Microbiological Research, 3(10), 658-663.
  6. Premraj R. and Doble M. (2005)., Biodegradation of polymers., IJBT, 4(2), 186-193.
  7. Albertsson A.C., Andersson S. and Karlsson S. (1987)., The mechanisms of biodegradation of polyethylene., Polym. Degrad Stab., 18(1), 73-87.
  8. Pramila R. and Vijaya R.K. (2011)., Biodegradation of Low Density Polyethylene (LDPE) by fungi isolated from marine water- a SEM analysis., African J. of Microbio. Res., 5(28), 5013-5018.
  9. Alshehrei F. (2017)., Biodegradation of Low Density Polyethylene by Fungi Isolated from Red Sea Water., Int. J. Curr. Microbiol. App. Sci, 6(8), 1703-1709.
  10. Ojha N., Pradhan N., Singh S., Barla A., Shrivastava A., Khatua P., Rai V. and Bose S. (2017)., Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization., Scientific Reports, 39515, 7, 1-13.
  11. Raaman N., Rajitha N., Jayshree A. and Jegadeesh R. (2012)., Biodegradation of plastic by Aspergillus spp. isolated from polythene polluted sites around Chennai., J. Acad. Indus. Res., 1(6), 313-316.
  12. Esmaeili A., Pourbabaee A. A., Alikhani H. A., Shabani F. and Esmaeili E. (2013)., Biodegradation of Low-Density Polyethylene (LDPE) by mixed culture of Lysinibacillus xylanilyticus and Aspergillus niger in soil., PLoS ONE, 8(9), e71720.
  13. Albertsson A.C. and Karlsson S. (1990)., The influence of biotic and abiotic environments on the degradation of polyethylene., Progress in Polymer Science, 15, 177-192.
  14. Ohtake Y., Kobayashi T., Murakami N. and Ono K. (1998)., Oxidative degradation and molecular weight change of LDPE buried under bioactive soil for 32-37 years., Journal of Applied Polymer Science, 70, 1643-1648.
  15. Bonhomme S., Cuer A., Delort A.M., Lemaire J., Sancelme M. and Scott G. (2003)., Environmental biodegradation of polyethylene., Polymer Degradation and Stability, 81(3), 441-452.
  16. Kathiresan K. (2003)., Polythene and plastics-degrading microbes from the mangrove soil., Revista de Biological Tropical, 51(3-4), 629-633.
  17. Orhan Y., Hrenovic J. and Buyukgungor H. (2004)., Biodegradation of plastic compost bags under controlled soil conditions., Acta Chimica Slovenica, 51(3), 579-588.
  18. Reddy R.M. (2008)., Impact of soil composting using municipal solid waste on biodegradation of plastics., Indian Journal of Biotechnology, 7, 235-239.
  19. Das M.P. and Kumar S. (2015)., An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens., Biotech, 5, 81-86.
  20. Report (2001)., Statistics of Foreign Trade of India., DGFT, GoI.
  21. Ghosh S.K., Pal S. and Ray S. (2013)., Study of microbes having potentiality for biodegradation of plastics., Environmental Science and Pollution Research, 20, 4339-4355.
  22. Kumari K., Aanad R.C. and Narula N. (2009)., Microbial degradation of Polyethylene (PE)., The South Pacific Journal of Natural Science, 27, 66-70.
  23. Hadad D., Geresh S. and Sivan A. (2005)., Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis., Appl. Microbio., 98(5), 1093-1100.
  24. Shah A.A., Hasan F., Hameed A. and Akhter J. (2009)., Isolation of Fusarium sp AF4 from sewage sludge, with the ability to adhere the surface of polyethylene., African Journal of Microbiological Research, 3(10), 658-663.
  25. Premraj R. and Doble M. (2005)., Biodegradation of polymers., IJBT, 4(2), 186-193.
  26. Albertsson A.C., Andersson S. and Karlsson S. (1987)., The mechanisms of biodegradation of polyethylene., Polym. Degrad Stab., 18(1), 73-87.
  27. Pramila R. and Vijaya R.K. (2011)., Biodegradation of Low Density Polyethylene (LDPE) by fungi isolated from marine water- a SEM analysis., African J. of Microbio. Res., 5(28), 5013-5018.
  28. Alshehrei F. (2017)., Biodegradation of Low Density Polyethylene by Fungi Isolated from Red Sea Water., Int. J. Curr. Microbiol. App. Sci, 6(8), 1703-1709.
  29. Ojha N., Pradhan N., Singh S., Barla A., Shrivastava A., Khatua P., Rai V. and Bose S. (2017)., Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization., Scientific Reports, 39515, 7, 1-13.
  30. Raaman N., Rajitha N., Jayshree A. and Jegadeesh R. (2012)., Biodegradation of plastic by Aspergillus spp. isolated from polythene polluted sites around Chennai., J. Acad. Indus. Res., 1(6), 313-316.
  31. Esmaeili A., Pourbabaee A. A., Alikhani H. A., Shabani F. and Esmaeili E. (2013)., Biodegradation of Low-Density Polyethylene (LDPE) by mixed culture of Lysinibacillus xylanilyticus and Aspergillus niger in soil., PLoS ONE, 8(9), e71720.
  32. Albertsson A.C. and Karlsson S. (1990)., The influence of biotic and abiotic environments on the degradation of polyethylene., Progress in Polymer Science, 15, 177-192.
  33. Ohtake Y., Kobayashi T., Murakami N. and Ono K. (1998)., Oxidative degradation and molecular weight change of LDPE buried under bioactive soil for 32-37 years., Journal of Applied Polymer Science, 70, 1643-1648.
  34. Bonhomme S., Cuer A., Delort A.M., Lemaire J., Sancelme M. and Scott G. (2003)., Environmental biodegradation of polyethylene., Polymer Degradation and Stability, 81(3), 441-452.
  35. Kathiresan K. (2003)., Polythene and plastics-degrading microbes from the mangrove soil., Revista de Biological Tropical, 51(3-4), 629-633.
  36. Orhan Y., Hrenovic J. and Buyukgungor H. (2004)., Biodegradation of plastic compost bags under controlled soil conditions., Acta Chimica Slovenica, 51(3), 579-588.
  37. Reddy R.M. (2008)., Impact of soil composting using municipal solid waste on biodegradation of plastics., Indian Journal of Biotechnology, 7, 235-239.
  38. Das M.P. and Kumar S. (2015)., An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens., Biotech, 5, 81-86.
  39. Chonde Sonal G., Chonde Sachin G. and Raut P.D. (2013)., Studies on degradation of synthetic polymer Nylon 6 and Nylon 6, 6 by Pseudomonas aeruginosa NCIM 2242., IJETCAS, 4, 362-369.
  40. Chonde S.G., Chonde S.G., Bhosale P.R., Nakade D.B. and Raut P.D. (2012)., Studies on degradation of synthetic polymer Nylon 6 by fungus Trametes versicolor NCIM 1086., International Journal of Environmental Sciences, 2(4), 2435-2442.
  41. Otake Y., Kobayashi T., Ashabe H., Murakami N. and Ono K. (1995)., Biodegradation of low density polyethylene, polystyrene, polyvinyl-chloride, and urea-Formaldehyde resin buried under soil for over 32 years., Journal of Applied Polymer Science, 56(13), 1789-1796.
  42. Sudhakar M., Doble M., Murthy P.S. and Venkatesan R. (2008)., Marine microbe-mediated biodegradation of low-and high-density polyethylenes., International Biodeterioration & Biodegradation, 61(3), 203-213.
  43. Soni R., Kapri A., Zaidi M.G.H. and Goel R. (2009)., Comparative biodegradation studies of non-poronized and poronized LDPE using indigenous microbial consortium., Journal of Polymers and the Environment, 17(4), 233.
  44. Gulmine J.V., Janissek P.R., Heise H.M. and Akcelrud L. (2002)., Polyethylene characterization by FTIR., Polymer Testing, 21(5), 557-563.
  45. Krimm S., Liang C.Y. and Sutherland G.B.B.M. (1956)., Infrared Spectra of High Polymers, II. Polyethylene., The Journal of Chemical Physics, 25(3), 549-562.Chonde Sonal G., Chonde Sachin G. and Raut P.D. (2013).
  46. Chonde S.G., Chonde S.G., Bhosale P.R., Nakade D.B. and Raut P.D. (2012)., Studies on degradation of synthetic polymer Nylon 6 by fungus Trametes versicolor NCIM 1086., International Journal of Environmental Sciences, 2(4), 2435-2442.
  47. Otake Y., Kobayashi T., Ashabe H., Murakami N. and Ono K. (1995)., Biodegradation of low density polyethylene, polystyrene, polyvinyl-chloride, and urea-Formaldehyde resin buried under soil for over 32 years., Journal of Applied Polymer Science, 56(13), 1789-1796.
  48. Sudhakar M., Doble M., Murthy P.S. and Venkatesan R. (2008)., Marine microbe-mediated biodegradation of low-and high-density polyethylenes., International Biodeterioration & Biodegradation, 61(3), 203-213.
  49. Soni R., Kapri A., Zaidi M.G.H. and Goel R. (2009)., Comparative biodegradation studies of non-poronized and poronized LDPE using indigenous microbial consortium., Journal of Polymers and the Environment, 17(4), 233.
  50. Gulmine J.V., Janissek P.R., Heise H.M. and Akcelrud L. (2002)., Polyethylene characterization by FTIR., Polymer Testing, 21(5), 557-563.
  51. Krimm S., Liang C.Y. and Sutherland G.B.B.M. (1956)., Infrared Spectra of High Polymers, II. Polyethylene., The Journal of Chemical Physics, 25(3), 549-562.