4th International Young Scientist Congress (IYSC-2018).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Banana peel as an inexpensive carbon source for microbial polyhydroxyalkanoate (PHA) production

Author Affiliations

  • 1Department of Biotechnology, REVA University, Rukmini Knowledge Park, Kattigenahalli, Bangalore- 560064, India
  • 2Parul Institute of Applied Sciences (PIAS), Parul Unviersity, Post Limda, Vadodara, Waghodia, Gujarat- 391760, India

Int. Res. J. Environment Sci., Volume 7, Issue (1), Pages 28-36, January,22 (2018)

Abstract

Polluted soil and water samples were used to isolate fourteen different bacterial isolates out of which four bacteria showed the ability to produce Polyhydroxyalkanoates. Banana peel was added to the media in different ratios as a sole carbon source. Polymer producers determined using 16s rRNA studies were found to be Staphylococcus aureus JH1, Geobacillus stearothermophilus R- 35646, Bacillus subtilis JCM 1465 and Bacillus siamensis PD- A10. Cupriavidus necator a reference bacterium for PHA production served as a positive control and the results obtained were 79.73%, 75.94% and 74.69% with C: N ratio of 3:1, 1:1 and 4:1 respectively upto 96hours of incubation. Geobacillus stearothermophilus R- 35646 produced 84.63%, 71.58% and 62.68% of PHA with 4:1, 3:1 and 2:1 C:N ratios respectively upto 96hours of incubation. Bacillus subtilis JCM 1465 accumulated 71.78% and 62.23% of PHA with 4:1 and 3:1 C:N ratios respectively at 24hours of incubation. Bacillus siamensis PD- A10 accumulated 77.55% at 24hours, 69.70% at 72hours and 65.75% at 72hours of incubation with 3:1, 3:1 and 4:1 C:N ratios respectively. While Staphylococcus aureus JH1 showed PHA accumulation of about 70.02% at 24hours and 52.74% at 48hours of incubation with 2:1 and 4:1 C:N ratios respectively.

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