Allergenic potential of two species of Poaceae: Panicum maximum Jacq and Sacciolepis africana C.E. Hubb pollen protein in albino mice
- 1Palynology Unit, Department of Biological Science, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
- 2Palynology Unit, Department of Botany, Faculty of Science, University of Lagos, Nigeria
- 3Clinical Pathology Department, Nigerian Institute of Medical Research,Yaba, Lagos, Nigeria
Int. Res. J. Environment Sci., Volume 6, Issue (10), Pages 1-5, October,22 (2017)
Pollen grains are the major cause of seasonal allergic rhinitis which affect millions of people worldwide. The presence of protein and glycoprotein content of their sporoderm and cytoplasm cause allergic sensitization in hypersensitive individuals. Immune system overreact by producing immunoglobulin E (IgE) antibodies which travel to cells that release allergic mediators causing allergic reactions. The objectives of this study were to; determine the concentration of pollen protein in two species of Poaceae: Panicum maximum and Sacciolepis africana, evaluate the level of immunoglobulin E (IgE) antibodies and immune cells of mice elicited by both pollen proteins. Pollen protein were extracted in 100 ml of 0.02 M of phosphate buffered saline (PBS) at pH 7.4 and protein content assayed. Albino mice were sensitized by two subcutaneous and one intranasal injections weekly for four weeks. Blood samples were obtained by retro-orbital bleedings, sera obtained were used for IgE evaluation by immuno assay. The result revealed a pollen protein contents of 420.27 µg/ml and 278.37 µg/ml in Panicum maximum and Sacciolepis africana respectively. Pollen protein of Panicum maximum induced a progressive change in the level of specific IgE in mice, after second sensitization. Both pollen proteins caused infiltration of basophil after first sensitization in albino mice. The result suggested that pollen which are dispersed from Panicum maximum and Sacciolepis africana are potential agents in inducing allergenic reaction. It also showed that Sacciolepis africana pollen protein are more potent than those of Panicum maximum. The research is the first study on pollen protein allergenicity in Nigeria.
- Izquierdo C.D.P., Piedons-Gutierrez B., Tellez H.D. and Prado O.Z.C. (2012)., Aerobiological study of Anemophilous pollens in the city of Toluca, Mexico., World Allergy Organization Journal, 5, 589-590.
- Wilson D.H., Adams R.J, Tucker G., Appleton S., Taylor A.W. and Ruffin R.E. (2006)., Trends in asthma prevalence and population changes in South Australia 1990-2003., Medical Journal of Australia, 184(5), 226-229.
- Asher M.I., Montefort S., Björkstén B., Lai C.K., Strachan D.P., Weiland S.K. and ISAAC Phase Three Study Group (2006)., Worldwide time trends in the prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and eczema in childhood: ISAAC Phases One and Three repeat multicountry cross-sectional surveys., Lancet, 368, 733-743.
- Khattab A. and Leventin E. (2008)., Effect of sampling height on the concentration of airborne fungal spores., Annals of Allergy, Asthma and Immunology, 101, 529-534.
- Taketomi E.A., Sopelete M.C., Moreira P.F.D.S. and Vieira F.D.A.M. (2006)., Pollen allergic disease: pollens and its major allergens., Revista Brasileira de Otorrinolaringologia, 72(4), 562-567.
- Huynen M. and Menne B. (2003)., Phenology and human health: allergic disorders., Report of a WHO meeting in Rome, Italy, 16-17 January 2003. Health and Global Environmental Series. EUR/03/5036791. World Health Organization, Copenhagen.
- Sharma D., Dutta B.K. and Singh A.B. (2009)., Biochemical and Immunological studies on eight pollen types from Assam, India., Iran Journal of Allergy, Asthma Immunology, 8(4), 185-192.
- Shahali Y., Majd A., Pourpak Z., Tajadod G., Haftlang M. and Moin M. (2007)., Comparative Study of the Pollen Protein Contents in Two Major Varieties of Cupressus arizonica Planted inTehran., Iran Journal of Allergy, Asthma and Immunology, 6(3), 123-127.
- D, Pollen‐related allergy in Europe., Allergy, 53(6), 567-578.
- Emberlin J., Jones S., Bailey J., Caulton E., Cordon J., Dubbels S., Evans J., Donagh N.M., Mullins J., Russel R. and Spencer T. (1994)., Variation in the start of the grass pollen season at selected sites in the United Kingdom 1987-1992., Grana, 33, 94-99.
- Mesa J.A.S., Smith M., Emberlin J., Allitt U., Caulton E. and Galan C. (2003)., Characteristics of grass pollen seasons in areas of southern Spain and the United Kingdom., Aerobiologia, 19, 243-250.
- Singh A.B. and Kumar P. (2004)., Aerial Pollen Diversity in India and Their Clinical Significance in Allergic Diseases., Indian Journal of Clinical Biochemistry, 19(2), 190-201.
- Bradford M.M. (1976)., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding., Annal of Biochemistry, 72, 248-258.
- Kuo J. (2007)., Processing plant tissues for ultrastructural study., Electron Microscopy: Methods and Protocols, 35-45.
- Riggion O., Montiel M., Fonseca J., Jaramillo O., Carvajah E., Rosenwaig P. and Colmenares A. (1994)., Type 1 hypersensitivity to gramineae pollen (by species in allergic rhinitis patients)., Revista de Biologia. Tropical, 42, 71-76.
- Yariktas M., Doner F., Dogru H., Yasan H. and Delibas N. (2004)., The role of free oxygen radicals on the development of otitis media with effusion., International Journal of Paediatric Otorhinolaryngology, 68(7), 889-894.
- Franco H., Falcone H.H. and Bernhard F.G. (2000)., The human Basophil: a new appreciation of its role in immune response., Blood Journal, 96, 4027-4038.
- Ye Y., Yang E., Yoo H., Shin Y., Kim S. and Park A. (2014)., Increased level of Basophil CD203 Expression predicts severe chronic urticarial., Journal of Korean Medical Science, 29(1), 43-47.