International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Identification and Characterization of Microbes from Industrial area for their Heavy metal Tolerance against Cadmium, Lead and Mercury

Author Affiliations

  • 1 Nitza Biologicals (P) Ltd., Secunderabad, 500056, Telangana, INDIA
  • 2 Siksha O Anusandhan University, Khandagiri, Bhubaneswar, 751003, Odisha, INDIA
  • 3 Trident Academy of Creative Technology, Patia, Bhubaneswar, 751024, Odisha, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (4), Pages 64-73, April,22 (2015)


The search for bacteria and fungus capable against metal toxicity starts with their isolation from the waste water released from the industries that uses either the heavy metals in their process or releases heavy metals as their waste product. The waste effluent released from paper, textile, paint and iron processing industries were collected and different microbial colonies were isolated from those waste water by standard plating methods, identified by their colony morphology, staining methods and different biochemical procedure. Those isolates were then screened for their antibiotics sensitivity and heavy metal toxicity test. From the antibiotics sensitivity test, Erythromycin and Streptomycin proved to be better antibiotics against isolated bacteria and Tetracycline and Ampicillin proved to be better against fungal isolates. Those antibiotics can be used as good selection markers in the molecular biology techniques. For heavy metal toxicity test, three heavy metals such as Cadmium, Mercury and Lead were analyzed at different concentrations such as 1mM, 5mM, 10mM and 20mM for up to 72 hours for bacterial isolates and 144 hours for fungal isolates. The potential isolates were selected over their growth rate at higher concentration of heavy metals. Bacterial isolates such as Bacillus megaterium, Bacillus licheniformis, Pseudomonas fluoroscence, Pseudomonas syringae, Bacillus subtilis, Corynebacterium xerosis, Bacillus macerans and fungal isolates such as Fusarium, Aspergillus niger, Aspergillus flavus, Cladosporium proved to be the better isolates that can be exploited at their molecular level for the bioremediation of heavy metal contamination.


  1. Szyczewski P., Siepak J., Niedzielski P. and Sobczyski T., Research on Heavy Metals in Poland, (2009)
  2. Vijendra singh and Singh Chandel C.P., Analytical Study of Heavy Metals of Industrial Effluents, at Jaipur, Rajasthan, (2006)
  3. Ahmad Varish, Dhama Gaurav, Singh Ajeet, Prakash Tej and Taqui Abbas, Isolation of potential pathogenic fungi from industrial effluent, (2011)
  4. Nanda Manisha, Sharma Dinesh and Arun Kumar, Removal of Heavy Metals from Industrial, Effluent Using Bacteria, (2011)
  5. Downes F.P. and Ito K.(Ed.), Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C, 2001 )
  6. Guinea J, Peláez T, Alcalá L, Bouza E, "Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia, Diagnostic microbiology and infectious disease, 53(4), 333–4 (2005)
  7. Gram, HC, Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten, Fortschritte der Medizin (in German), :185–189, English translation in:Brock, T.D. (1999) , Milestones in Microbiology 1546–1940 (2 ed.). ASM Press, 215–218 ISBN 1-55581-142-6, (1884)
  8. Bergey's Manual of Determinative Bacteriology, 9th EditionEdited by John G. Holt Copyright, Williams and Wilkins, Baltimore, ISBN 0-683-00603-7, (1999)
  9. Anderson N.L. et al., Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C, (2005)
  10. Murray P.R. et al., Manual of Clinical Microbiology, 8th ed. American Society for Microbiology, Washington, D.C, (2003)
  11. Forbes B.A. et al., Bailey and Scott's Diagnostic Microbiology, 12th ed. C.V. Mosby Company, St. Louis, MO, (2007)
  12. Isenberg H.D., Clinical Microbiology Procedures Handbook, I, II and III, American Society for Microbiology, Washington, D.C, (2007)
  13. James G.C. and Natalie S., Microbiology, Alaboratory Manual (ed.)., 211-223 (2001)
  14. Ríos J.L., Recio M.C., Villar A., Screening methods for natural products with antimicrobial activity: a review of the literature, Journal of ethnopharmacology, 23, 127–149 (1988)