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Isolation of Plastic Degrading Micro-organisms from Soil Samples Collected at Various Locations in Mumbai, India

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Author Affiliations

  • 1 Department of Biotechnology, Kishinchand Chellaram College, D.W. Road, Churchgate, Mumbai, INDIA
  • 2 Department of Life Sciences, Kishinchand Chellaram College, D.W. Road, Churchgate, Mumbai, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (3), Pages 77-85, March,22 (2015)

Abstract

Accumulation of plastics, especially Polyethylene terephthalate (PET) and Polystyrene (PS), is an ever increasing ecological threat due to its excessive usage in everyday human life. In contribution to regulate this potent ecological threat an attempt has been made to isolate plastic degrading micro-organisms from five different soil samples viz. Garden soil, Mangrove soil, Forest soil, soil near Petrol Pump, and Garbage soil. In the present study, it was found that after four months of incubation period the percentage loss in weight of PET and PS was highest in the Garden soil and Garbage soil respectively as compare to other soil samples in regards with Gram positive coccobacillus, Gram negative cocci, Gram negative rod shaped bacillus, Gram positive cocci (in clusters) in Garden soil and Gram negative cocci (in singles) in Garbage soil. In addition to this, the degradation rate of PET and PS by Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes, and Aspergillus Niger was also observed separately. It was also noted that the percentage loss in weight of PS was highest by Bacillus subtilis (in Nutrient broth and Bushnell Hass broth), whereas in the case of PET, percentage loss in the weight was highest by Bacillus subtilis in Nutrient broth, Pseudomonas aeruginosa in Bushnell Hass broth, and Aspergillus niger in Rose Bengal broth.

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