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Toxic Microcystis aeruginosa in Cyanobacterial blooms Collected in Botswana

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Author Affiliations

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Int. Res. J. Environment Sci., Volume 4, Issue (10), Pages 22-29, October,22 (2015)

Abstract

Cyanobacterial blooms are prevalent in surface water bodies in Botswana during summer months. Microscopic examination of cyanobacterial blooms from the Gaborone oxidation ponds showed the predominance of toxic Microcystis aeruginosa and contained up to 1.7 mg/g DW microcystin-LR. Cyanobacterial cells were raptured by freeze-thawing. The resulting cell suspension inhibited seed germination and seedling growth of two plants, Brassica nigra (cultivated) and Bidens pilosa (weed), in a concentration dependent manner. Complete inhibition of germination of B. pilosa seeds was observed with cell suspension containing 5 mg DW/ml. Pollen grains from Setcreasea pallida were also highly sensitive to the presence of toxic M. aeruginosa cells with complete inhibition observed when 0.3 mg DW/ml was incorporated into the growth media. Purified microcystin-LR was less toxic to the seeds of B. nigra and B. pilosa and to the pollen grains from S. pallida, suggesting that other factors from the freeze-thawed cells may have contributed to the toxicity of the cyanobacteria cells. However, these plants can be used to screen environmental samples containing M. aeruginosa as an initial assessment tool to test for toxicity of the cyanobacterial blooms.

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