International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Spectrophotometric Determination of Trace Amount of Nitrite in Water with 4-Aminophenylacetic Acid and Phloroglucinol

    ,

Author Affiliations

  • 1 Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NEPAL

Int. Res. J. Environment Sci., Volume 3, Issue (4), Pages 64-69, April,22 (2014)

Abstract

The developed method is simple, selective and sensitive and has been applied to various water samples for the determination of nitrite. This method is based on diazo coupling reaction, where 4-aminophenylacetic acid is reacted with nitrite in the presence of acid to form diazonium ion. This diazonium ion further reacted with phloroglucinol to form a stable yellow azo-dye. The formed azo-dye has absorption maxima at 420 nm. Various analytical parameters have been studied. The system obeys Lambert-Beer’s law in the range of 0.04-1.8 µg /ml. The correlation coefficient, molar absorptivity and Sandell’s sensitivity were found to be 0.998, 2.40×10 lit.mol-1cm-1 and 1.90×10-3 µg cm-2 respectively. Furthermore, the percentage recovery was found to be in the range of 92.9-103. Some of interference anion and cation was also tested. Although the interference due to foreign ions is negligible except copper and iron, the developed method should be further tested to eliminate such interfering ingredient in future.

References

  1. Henery J.G. and Heinke G.W., Environmental Science and Engineering, 2nd ed., Prentice Hall, New Jersey (1996)
  2. Cox R.D. and Frank C.W., Determination of nitrate and nitrite in blood and urine by chemiluminescence, J. Anal. Toxicol.,6(3), 148-152 (1982)
  3. Gabbay J., Almog Y., Davidson M. and Donagi A.E., Rapid spectrophotometric micro-determination of nitrites in water, Analyst, 102, 371-376 (1997)
  4. Manzoori J.L., Sorouraddin M.H. and Haji-Shabani A.M., Spectrophotometric determination of nitrite based on its catalytic effect on the oxidation of carminic acid by bromate, Talanta,46(6), 1379-1386 (1998)
  5. Dayananda B.P. and Revanasiddappa H.D., A facile analytical method for the determination of nitrite in water and soil samples, EJEAF Chem.,6(8), 2263-2271 (2007)
  6. Healeh M.I.H. and Korenaga T., Ion chromatographic method for simultaneous determination of nitrate and nitrite in human saliva, J. Chromatogr., 744(2), 433-437 (2000)
  7. Kage S., Kudo K. and Ikeda N., Determination of nitrate in blood by gas chromatography and gas chromatography-mass spectrometry, J. Chromatogr., 742, 363-368 (2000)
  8. Butt S.B., Riaz M. and Iqbal M.Z., Simultaneous determination of nitrite and nitrate by normal phase ion-pair liquid chromatography, Talanta,55(4), 789-797 (2001)
  9. Siu D.C. and Henshall A., Ion chromatographic determination of nitrite and nitrate in meat products, J. Chromatogr., 804, 157-160 (1998)
  10. Barzegar L., Rezaei Behbehani G., Moosavi M. and Mehreshtiagh M., A calorimetric study on the interaction between Vitamin-B6 and lysozyme, Res. J. Recent. Sci., 1(ISC-2011), 345-347 (2012)
  11. Nwajei G.E., Okwagi P., Nwajei R.I. and Obi-Iyeke G.E, Analytical assessment of trace elements in soils, tomato leaves and fruits in the vicinity of paint industry, Nigeria, Res. J. Recent. Sci., 1(4), 22-26 (2012)
  12. Chaitanya Lakshmi G., Food coloring: The natural way, Res. J. Chem. Sci., 4(2), 87-96 (2014)
  13. Bassett J., Denney R.C., Jeffery G.H. and Mendham J., Vogel's Text Book of Quantitative Analysis,th Ed. ELBS, LONGMAN, 447 (1978)
  14. Saltzman B.E.,Colorimetric microdetermination nitrogen dioxide in atmosphere, Anal. Chem., 26(12), 1949-1955 (1954)
  15. Cherian T. and Narayana B., A new system for the spectrophotometric determination of trace amounts of nitrite in environmental samples, J. Braz. Chem. Soc., 17(3), 577-581 (2006)
  16. Cotton F.A. and Wilkinson G., Advanced Inorganic Chemistry, 5th ed, John Wiley and Sons Inc. (1988)
  17. Pradhananga R.R., Khadka D.B. and Sherestha N.K., Spectrophotometric determination of trace amount of nitrite in water with 4-aminophenylacetic Acid and 1-naphthol, J. Nepal Chem. Soc., 11, 36-43 (1992)
  18. Veena K. and Narayana B., Spectrophotometric determination of nitrite using new coupling agents,Indian J. Chem. Technol., 16, 89-92 (2009)
  19. Flamerz S. and Bashir W.A., Spectrophotometric determination of nitrite in waters, Analyst,106, 243-247 (1981)
  20. Amin D., Determination of nitrite on using the reaction with 4-aminobenzotrifluoride and 1-naphthol, Analyst, 111, 1335-1337 (1986)
  21. Bashir W.A. and Flamerz S., Photometric determination of nitrite, Talanta,28(9), 697-699 (1981)