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Isolation and Biochemical Characterization of Chromium Reducing Bacteria from Hudiara Drain Sludge

Author Affiliations

  • 1Department of Environmental Science, Lahore College for Women University, Jail road, Lahore PAKISTAN

Int. Res. J. Environment Sci., Volume 3, Issue (4), Pages 42-50, April,22 (2014)

Abstract

In the past few decades, the environmental pollution of toxic heavy metals is the major issue throughout the world since industrial evolution. Heavy metals found in wastewater are harmful to the environment and their effects on biological system are very severe. Chromium is one of these heavy metals whose concentration in the environment is increasing due to different industrial processes. There are two inorganic forms, Cr (VI) and Cr (III), in which chromium exists in nature. Cr (VI) is a serious environmental pollutant. A variety of microorganisms have been known for their ability to degrade these heavy metals. The current study aimed to isolate chromium reducing microorganism from Hudiara drain sludge. Among all the species diversity in sludge two isolated species (SP1 and SP2) were selected for further analysis based on their morphological characteristics. The isolated strains were identified through biochemical characterization. The species were characterized according to criteria given in Bergey's Manual of Determinative Bacteriology (1984). The efficiency of two isolates for chromium reduction was also determined, which shows that SP1 show 65% reduction and SP2 show 53% reduction of chromium from the medium in 12 hours. This shows that Pseudomonas putida was efficient in reducing Cr (VI) than Pseudomonas plecoglossicida.

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