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Biodegradation of Phenol by a Bacterial Strain Isolated From a Phenol Contaminated Site in India

Author Affiliations

  • 1Department of Microbiology, Bhagwan Mahavir College of Biotechnology, Surat-394220, Gujarat, INDIA
  • 2 Department of Microbiology, Bhagwan Mahavir College of Biotechnology, Surat-394220, Gujarat, INDIA
  • 3 Department of Microbiology, Bhagwan Mahavir College of Biotechnology, Surat-394220, Gujarat, INDIA

Int. Res. J. Environment Sci., Volume 1, Issue (1), Pages 46-49, August,22 (2012)


Phenol, an organic compound, is very toxic upon ingestion, contact or inhalation and is lethal to living organism even at low concentration. Phenolic compounds are present in varying concentration in waste water of synthetic resins, plastic and polymer manufacturing, dye and dye intermediate manufacturing and pharmaceutical industries. Among various techniques available for removing phenol biological treatment has been proved to be economical and most promising and versatile approach. The present investigation was under taken to assess the biodegradation of phenol by native bacterial strain from effluent from phenol contaminated site of Amla Khadi, Ankleshwar. A selected, Gram positive, bacterial strain so isolated, has been used to study biodegradation of phenol in shake flask culture. Various physicochemical parameters are optimized for the maximum biodegradation of phenol, viz., pH, temperature, initial concentration of phenol, additional carbon sources and additional nitrogen sources. Complete phenol biodegradation was achieved after 4 days in 1000 ppm solution. The isolated Gram positive bacterium can be exploited as a candidate of choice for the bioremediation of phenolic effluent.


  1. Kumar Praveen G.N. and Sumangala K.B., FungalDegradation of Azo dye- Red 3BN and Optimization of Physico-Chemical Parameters, ISCA Journal of Biological Sciences, 1(2), 17-24 (2012)
  2. Muftah H.E., Shaheen A.A. and Souzan M., Biodegradation of phenol by Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel, Journal of hazardous material, 164, 720-725 (2009)
  3. Hayashi D., Hoeben W.F.L.M., Veldhuizen E.M., Rutgers W.R. and Kroeson G.M.W., In-situ study for the reaction pathway of aqueous phenol degradation by pulsed corona discharges. Proceedings of international conference on phenomena in ionized gases, Germany (2003)
  4. Kumar S.R., Kumar R. S. and Kumar R. J., Decolorization of Reactive Black HFGR by Aspergillus sulphureus, ISCA Journal of Biological Sciences, 1(1), 55-61 (2012)
  5. Baroniya M., Baroniya S.S. and Jain M., Operation and Maintenance of Water Treatment Plant at BNP Campus Dewas, India: A Case Study, ISCA Journal of Biological Sciences, 1(1), 83-86 (2012)
  6. Murhekar G.H., Trace Metals Contamination of Surface Water Samples in and Around Akot City in Maharashtra,India, Research Journal of Recent Sciences, 1(7), 5-9 (2012)
  7. Nor S.Y., Ariff A., Rosfarizan M., Ahmed S.A., Abdul L. I.,Norazah M.N. and Shukor M. Y. A., Optimization of parameter for phenol degradation by Rhodococcus UKM-Pin shake flask culture, Proceedings of the World Congress on Engineering, 1, (2010)
  8. Pawar M.J., Nimbalkar V.B., Synthesis and phenol degradation activity of Zn and Cr doped TiO2 Nanoparticles, Research Journal of Chemical Sciences, 2(1), 32-37 (2012)
  9. Frieda O. and Nava N., Characteristics of organic removal by PACT simultaneous adsorption and biodegradation,Water Research, 31(3), 391-398 (1997)
  10. Ruey S.J., Wen C.H. and Ya H.H., Treatment of phenol in synthetic saline wastewater by solvent extraction and two phase membrane biodegradation, Journal of Hazardous Materials, 164(1), 46-52 ( 2009)
  11. Wang Y.T., Effect of chemical oxidation on anaerobicbiodegradation of model phenolic compounds, Water Environment Research, 64(3), 268-273 (1992)
  12. Azin I. and Katayon S., Degradation of phenol in wastewater using anolyte produced from electrochemical generation of brine solution, Global Nest: International journal, 4(2-3), 139-144 (2002)
  13. Sally N.J., Extraction of Phenol from Industrial Water Using Different Solvents, Research Journal of Chemical Sciences,2(4), 1-12 (2012)
  14. Pichiah S., Kannan A.P. and Prabirkumar S., Kinetics of phenol and m-cresol biodegradation by an indigenous mixed microbial culture isolated from a sewage treatment plant,Journal of Environmental Sciences. 20, 1508–1513 (2008)
  15. Garrity G.M., Bergey’s Manual of Systematic Bacteriology,part B 2nd Verlag, 2 (2005)
  16. Hank D., Saidani N., Namane A. and Hella A., Batch phenol biodegradation study and application of factorial experimental design, Journal of Engeneering Science and Tecnology Review, 3(1), 123-127 (2010)
  17. Indu C.N. and Shankar S., Microbial degradation of phenol by a species of Alcaligenes isolated from a tropical soil,Ipyhmoehabcmbo, 5(3-4), 47-51 (2004)
  18. Martin R.W., Rapid Colorimetric estimation of phenol,Analytical chemistry, 21(11), 1419-1420 (1949)