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Screening of amylase producing bacteria from soil samples of evergreen and deciduous forest

Author Affiliations

  • 1Department of Biotechnology and Research, K.V.M. College of Science and Technology, Kokkothamangalam P. O., Cherthala-688583, Alappuzha District, Kerala, India
  • 2National College of Arts and Science, Kallattumukku, Manacaud, Thiruvananthapuram, Kerala, India
  • 3Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram, Kerala, India
  • 4Department of Biotechnology and Research, K.V.M. College of Science and Technology, Kokkothamangalam P. O., Cherthala-688583, Alappuzha District, Kerala, India

Int. Res. J. Biological Sci., Volume 8, Issue (6), Pages 1-6, June,10 (2019)


Amylase is an industrially important enzyme used for the hydrolysis of dietary starch into disaccharides and trisaccharides and ultimately to glucose. Plants, animals and certain microorganisms produce this amylase and are used for various biochemical reactions. The most stable and reliable source of amylase is obtained from microbes when compared to other sources. The purpose of the current study was to isolate and characterize amylase producing bacteria from soil samples collected from the evergreen and deciduous forest of Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram, Kerala, India. The bacterial colonies were isolated through serial dilution and plating techniques. One bacterial colony which showed the maximum zone of clearance in starch agar plates were isolated and cultured separately. The screening was done on the basis of hydrolysis of starch by amylase on agar plate containing 1% starch. The 16S rDNA sequence of the selected strain was isolated and screened using Clustal W programme and a phylogenetic tree was constructed using a Mega 6 software using neighbor joining method. The bacteria were identified as Bacillus sps.


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