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Seasonal variation in bacterial diversity of Tuva Timba thermal springs of Gujarat, India

Author Affiliations

  • 1Department of Life Sciences, Gujarat University, Ahmedabad, India and Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 2Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 3Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 4Department of Life Sciences, Gujarat University, Ahmedabad, India

Int. Res. J. Biological Sci., Volume 8, Issue (2), Pages 6-14, February,10 (2019)

Abstract

The seasonal bacterial diversity study of thermophilic isolated from TuvaTimba hot springs in terms of morphological, physicochemical, biochemical tests and molecular characterization. A total of 48 isolates were cultivated using various media. Out of these isolates 25 were Gram-positive, 19 Gram-negative and 4 were Gram variable. Based on the metabolic activity of these isolates, 29 could produce catalase, 45, 47 and 24 utilized casein, starch, and citrate respectively. 22 produced H2S by sulphate reduction. Sugar utilization varied in all the isolates. Diversity indices such as Simpson, Shanon, Menhinick and Margalef index were also studied based on their metabolic activity. 25 well isolated purified colonies were selected for molecular analysis. Based on this study the identified isolates belonged to genera Bacillus, Brevibacillus, Geobacillus, Anoxybacillus and Brevibacterium. All these isolates may have potential biotechnological and industrial application.

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