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Seasonal variation in bacterial diversity of Tuva Timba thermal springs of Gujarat, India

Author Affiliations

  • 1Department of Life Sciences, Gujarat University, Ahmedabad, India and Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 2Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 3Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India
  • 4Department of Life Sciences, Gujarat University, Ahmedabad, India

Int. Res. J. Biological Sci., Volume 8, Issue (2), Pages 6-14, February,10 (2019)


The seasonal bacterial diversity study of thermophilic isolated from TuvaTimba hot springs in terms of morphological, physicochemical, biochemical tests and molecular characterization. A total of 48 isolates were cultivated using various media. Out of these isolates 25 were Gram-positive, 19 Gram-negative and 4 were Gram variable. Based on the metabolic activity of these isolates, 29 could produce catalase, 45, 47 and 24 utilized casein, starch, and citrate respectively. 22 produced H2S by sulphate reduction. Sugar utilization varied in all the isolates. Diversity indices such as Simpson, Shanon, Menhinick and Margalef index were also studied based on their metabolic activity. 25 well isolated purified colonies were selected for molecular analysis. Based on this study the identified isolates belonged to genera Bacillus, Brevibacillus, Geobacillus, Anoxybacillus and Brevibacterium. All these isolates may have potential biotechnological and industrial application.


  1. Adiguzel A., Ozkan H., Baris O., Inan K., Gulluce M. and Sahin F. (2009)., Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey., Journal of microbiological methods, 79(3), 321-328.
  2. Khuntia H.K., Suryakanta S., Sahoo R.K., Kar S.K., Pal B.B. and Bihari K.M. (2010)., Isolation and characterization of thermophilic bacteria from hot spring in Orissa, India., Biosciences, Biotechnology Research Asia, 7(1), 449-451.
  3. Krıstjonsson J.K. and Stetter K.O. (1991)., Thermophilic Bacteria Thermophilic Bacteria (Ed: Kristjonsson JK) CRC Pres., Inc. London, 1-13.
  4. Baltaci M.O., Genc B., Arslan S., Adiguzel G. and Adiguzel A. (2017)., Isolation and characterization of thermophilic bacteria from geothermal areas in Turkey and preliminary research on biotechnologically important enzyme production., Geomicrobiology journal, 34(1), 53-62. DOI: 10.1080/01490451.1137662.2015.
  5. Adiguzel A., Inan K., Sahin F., Ozbek T., Gulluce M., Belduz A.O. and Baris O. (2011)., Molecular Diversity of Thermophilic Bacteria Isolated From Erzurum City Pasinler Hot Spring (Erzurum/Turkey)., Turkish Journal of Biology, 35(3), 267-274.
  6. Genc B., Nadaroglu H., Adiguzel A. and Baltaci O. (2015)., Purification and characterization of an extracellular Cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey., Journal of Environmental Biology, 36(6), 1319-1324.
  7. Sharma A., Pandey A., Shouche Y.S., Kumar B. and Kulkarni G. (2009)., Characterization and identification of Geobacillus spp. isolated from Soldhar hot spring site of Garhwal Himalaya, India., Journal of basic microbiology, 49(2), 187-194.
  8. Rath C.C. and Subramanyam V.R.J. (1996). Microbes. 86, 157-161., undefined, undefined
  9. Panda S.K., Jyoti V., Bhadra B., Nayak K.C., Shivaji S., Rainey F.A. and Das S.K. (2009)., Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium., International journal of systematic and evolutionary microbiology, 59(9), 2171-2175.
  10. Fooladi J. and Sajjadian A. (2009)., Screening the thermophilc and hyperthermophilic bacterial population of three Iranian hot springs to detect thermostable amylase producing bacteria., Iranian Journal of Microbiology, 2(1), 40-53.
  11. Mageswari A., Subramanian P., Chandrasekaran S., Sivashanmugam K., Babu S. and Gothandam K. M. (2012)., Optimization and immobilization of amylase obtained from halotolerant bacteria isolated from solar salterns., Journal of Genetic Engineering and Biotechnology, 10(2), 201-208.
  12. Indriati G. and Megahati R.R.P. (2018)., Isolation of Thermophilic Bacteria and Optimizing the Medium Growth Conditions., Int. J. Curr. Microbiol. App. Sci, 7(1), 1457-1464.
  13. Scow K.M., Schwartz E., Johnson M.J. and Macalady J.L. (2001)., Microbial biodiversity, measurement of. Levin., SA, Editor, 177-190.
  14. Cai H., Archambault M. and Prescott J.F. (2003)., 16S Ribosomal RNA Sequence-Based Identification of Veterinary Clinical Bacteria., Journal of Veterinary Diagnostic Investigation, 15(5), 465-469.
  15. Clarridge J.E. (2004)., Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases., Clinical microbiology reviews, 17(4), 840-862.
  16. Mignard S. and Flandrois J.P. (2006)., 16S rRNA sequencing in routine bacterial identification: a 30-month experiment., Journal of microbiological methods, 67(3), 574-581.
  17. Miller S.R., Purugganan M.D. and Curtis S.E. (2006)., Molecular population genetics and phenotypic diversification of two populations of the thermophilic cyanobacterium Mastigocladus laminosus., Applied and environmental microbiology, 72(4), 2793-2800.
  18. Boomer S.M., Noll K.L., Geesey G.G. and Dutton B.E. (2009)., Formation of multilayered photosynthetic biofilms in an alkaline thermal spring in Yellowstone National Park, Wyoming., Applied and environmental microbiology, 75(8), 2464-2475.
  19. Sievert S.M., Ziebis W., Kuever J. and Sahm K. (2000)., Relative abundance of Archaea and Bacteria along a thermal gradient of a shallow-water hydrothermal vent quantified by rRNA slot-blot hybridization., Microbiology, 146(6), 1287-1293.
  20. Maugeri T.L., Gugliandolo C., Caccamo D. and Stackebrandt E. (2001)., A polyphasic taxonomic study of thermophilic bacilli from shallow, marine vents., Systematic and Applied Microbiology. 24(4), 572-587.
  21. Takacs C.D., Ehringer M., Favre R., Cermola M., Eggertsson G., Palsdottir A. and Reysenbach A.L. (2001)., Phylogenetic characterization of the blue filamentous bacterial community from an Icelandic geothermal spring., FEMS Microbiology Ecology, 35(2), 123-128.
  22. Atanassova M., Derekova A., Mandeva R., Sjøholm C. and Kambourova M. (2008)., Anoxybacillus bogrovensis sp. nov., a novel thermophilic bacterium isolated from a hot spring in Dolni Bogrov, Bulgaria., International journal of systematic and evolutionary microbiology, 58(10), 2359-2362.
  23. Derekova A., Mandeva R. and Kambourova M. (2008)., Phylogenetic diversity of thermophilic carbohydrate degrading bacilli from Bulgarian hot springs., World Journal of Microbiology and Biotechnology, 24(9), 1697-1702.
  24. Lau M.C., Aitchison J.C. and Pointing S.B. (2009)., Bacterial community composition in thermophilic microbial mats from five hot springs in central Tibet., Extremophiles, 13(1), 139-149.
  25. Akkaya S.E. and Kivanc M. (2009)., Thermophilic Bacteria; Methods of isolation and identification of Gram-negative Bacilli in hot springs., Electronic Microbiology Journal, 07(1), 01-23.
  26. Hammer R., Harper D. and Ryan P. (2001)., PAST : Paleontological Statistics Software Packages for Education and Data Analysis., Palaeontologia Electronica, 4, 9.
  27. Moore Edward and Angelika Arnscheidt (2004)., Simplified Protocols for the Preparation of Genomic DNA from Bacterial Cultures Simplified Protocols for the Preparation of Genomic DNA from Bacterial Cultures., Molecular Microbial Ecology Manual, Second Edition, 1.01, 3-18.
  28. Wilson K. (1987)., Preparation of genomic DNA from bacteria., 2.4. 1-2.4. 5 in FM Ausubel, R. Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, K. Struhl, eds. Current protocols in molecular biology. John Wiley & Sons, New York, NY.
  29. Joshi S.J., Suthar H., Yadav A.K., Hingurao K. and Nerurkar A. (2012)., Occurrence of biosurfactant producing Bacillus spp. in diverse habitats., ISRN biotechnology, 2013, 6. 652340.
  30. Gohel H.R., Ghosh S.K. and Bragazna V.J. (2013)., Production, Purification and Immobilization of Extracellular Lipases Xrf11 and Bacillus Licheniformis Xrf12 for Production of Alkyl Esters., International Journal of Life Sciences Biotechnology and Pharma Research., 2(3), 278-286.
  31. Dudhagara P., Bhavasar S., Ghelani A. and Bhatt S. (2014)., Isolation, characterization and investing the industrial applications of thermostable and solvent tolerant serine protease from hot spring isolated thermophililic Bacillus licheniformis U1., International Journal of Applied Sciences and Biotechnology, 2(1), 75-82.
  32. Kikani B.A. and Singh S.P. (2011)., Single step purification and characterization of a thermostable and calcium independent -amylase from Bacillus amyloliquifaciens TSWK1-1 isolated from Tulsi Shyam hot spring reservoir, Gujarat (India)., International Journal of Biological Macromolecules, 48(4), 676-681.
  33. Ferris M.J. and Ward D.M. (1997)., Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis., Applied and environmental microbiology, 63(4), 1375-1381.
  34. Mackenzie R., Pedrós-Alió C. and Díez B. (2013)., Bacterial composition of microbial mats in hot springs in Northern Patagonia: variations with seasons and temperature., Extremophiles, 17, 123-136.
  35. Brandon R. Briggs, Eoin L. Brodie, Lauren M. Tom, Hailiang Dong, Hongchen Jiang, Qiuyuan Huang, Shang Wang, Weiguo Hou, Geng Wu, Liuquin Huang, Brian P. Hedlund, Chuanlun Zhang, Paul Dijkstra and Bruce A. Hungate. (2014)., Seasonal Patterns in Microbial Communities Inhabiting the Hot Springs of Tengchong, Yunnan Province, China., Environmental Microbiology, 16(6), 1579-1591.
  36. Prieto-Barajas C.M., Alfaro-Cuevas R., Valencia-Cantero E. and Santoyo G. (2017)., Effect of seasonality and physicochemical parameters on bacterial communities in two hot spring microbial mats from Araró, Mexico., Revista Mexicana de Biodiversidad, 88(3), 616-624.
  37. Archna S., Priyank V., Nath Y.A. and Kumar S.A. (2015)., Bioprospecting for extracellular hydrolytic enzymes from culturable thermotolerant bacteria isolated from Manikaran thermal springs., Research Journal of Biotechnology, 10(4), 33-42.
  38. Huang Q., Dong C.Z., Dong R.M., Jiang H., Wang S., Wang G., Bin Fang, Xiaoxue Ding, Lu Niu, Xin Li, Chuanlun Zhang and Hailiang Dong (2011)., Archaeal and Bacterial Diversity in Hot Springs on the Tibetan Plateau, China., Extremophiles, 15(5), 549-563.
  39. Khiyami Mohammad A., Ehab A. Serour, Maher M. Shehata and Ali H. Bahklia (2012)., Thermo-Aerobic Bacteria from Geothermal Springs in Saudi Arabia., African Journal of Biotechnology, 11(17), 4053-4062. et al.htm.
  40. Sahoo R.K., Subudhi E. and Kumar M. (2015)., Investigation of bacterial diversity of hot springs of Odisha, India., Genomics Data, 6, 188-190.
  41. Sen R. and Maiti N.K. (2014)., Genomic and functional diversity of bacteria isolated from hot springs in Odisha, India., Geomicrobiology Journal, 31(7), 541-550.
  42. Stefanova K., Tomova I., Tomova A., Radchenkova N., Atanassov I. and Kambourova M. (2016)., Archaeal and bacterial diversity in two hot springs from geothermal regions in Bulgaria as demostrated by 16S rRNA and GH-57 genes., International Microbiology, 18(4), 217-223.
  43. Yadav A.N., Verma P., Kumar M., Pal K.K., Dey R., Gupta A, Jasdeep Chatrath Padaria, Govind T. Gujar, Sudheer Kumar, Archna Suman, Radha Prasanna and Anil K. Saxena (2015)., Diversity and Phylogenetic Profiling of Niche-Specific Bacilli from Extreme Environments of India., Annals of Microbiology, 65(2), 611-629.
  44. Sahay H., Yadav A.N., Singh A.K., Singh S., Kaushik R. and Saxena A.K. (2017)., Hot Springs of Indian Himalayas : Potential Sources of Microbial Diversity and Thermostable Hydrolytic Enzymes., 3 Biotech., 7(2), 1-11.
  45. Kiel G. and Gaylarde C.C. (2007)., Diversity of salt-tolerant culturable aerobic microorganisms on historic buildings in Southern Brazil., World Journal of Microbiology and Biotechnology, 23(3), 363-366.
  46. Kikani B.A., Sharma A.K. and Singh S.P. (2015)., Culture dependent diversity and phylogeny of thermophilic bacilli from a natural hot spring reservoir in the Gir Forest, Gujarat (India)., Microbiology, 84(5), 687-700.
  47. Schallmey M., Singh A. and Ward O.P. (2004)., Developments in the use of Bacillus species for industrial production., Canadian Journal Microbiology, 50(1), 1-17. PMid:15052317. http://
  48. Inan K., Canakci S., Belduz A.O. and Sahin F. (2012)., Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring., International Journal of Systematic and Evolutionary Microbioogy, 62, 849-855.
  49. Baek S.H., Im W.T., Oh H.W., Lee J.S., Oh H.M. and Lee S.T. (2006)., Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field., International journal of systematic and evolutionary microbiology, 56(11), 2665-2669.
  50. Choi M.J., Bae J.Y., Kim K.Y., Kang H. and Cha C.J. (2010)., Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland., International journal of systematic and evolutionary microbiology, 60(7), 1595-1599.
  51. Song Jinlong, Wang Yanwei, Song Yi, Zhao Bingqiang, Wang Huimin, Zhou Shan, Kong Delong, Guo Xiang, Li Yanting, He Mingxiong, Ma Kedong, Ruan Zhiyong and Yan Yanchun (2017)., Brevibacillus Halotolerans Sp. Nov., Isolated from Saline Soil of a Paddy Field., International Journal of Systematic and Evolutionary Microbiology, 67(4), 772-777.
  52. Aanniz T., Ouadghiri M., Melloul M., Swings J., Elfahime E., Ibijbijen J. and Amar M. (2015)., Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils., Brazilian Journal of Microbiology, 46(2), 443-453.
  53. Hadad D., Geresh S. and Sivan A. (2005)., Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis., Journal of applied microbiology, 98(5), 1093-1100.
  54. Rath K., Mishra B. and Vuppu S. (2012)., Biodegrading ability of organo-sulphur compound of a newly isolated microbe Bacillus sp. KS1 from the oil contaminated soil., Archives of Applied Science Research, 4(1), 465-471.
  55. Babu G.P., Subramanyam P., Sreenivasulu B. and Paramageetham C. (2014)., Isolation and identification of sulfate reducing bacterial strains indigenous to sulphur rich barite mines., International Journal of Current Microbiology and Applied Sciences, 3(7), 788-793.