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Piperine production from endophytic fungi of Piper nigrum l and its In Silico approach for anti-inflammatory and anti-mycobacterial potential

Author Affiliations

  • 1Department of Biotechnology, Reva Institute of Science and Management, Banglore–560064, India
  • 2Department of Biotechnology, Reva Institute of Science and Management, Banglore–560064, India
  • 3Department of Biotechnology, Reva Institute of Science and Management, Banglore–560064, India
  • 4Department of Biotechnology, Reva Institute of Science and Management, Banglore–560064, India
  • 5Centre for R&D Life Sciences, Department of Biotechnology, Dayananda Sagar College of Engineering, Banglore-78, India

Int. Res. J. Biological Sci., Volume 8, Issue (12), Pages 8-15, December,10 (2019)


Present study was aimed at culturing endophytic fungi Colletotrichum gloeosporioides from Piper nigrum L. and extracting alkaloid piperine from submerged fermentation of isolated fungal endophyte. We also aimed for an in silico approach on anti-mycobacterial and anti-inflammatory role of piperine. Stems of Piper nigrum L. were spread plated on fungal selective media (Arginine glycerol media; AGA), and hyphae from identified fungal culture were subjected for submerged fermentation in Martin′s Rose Bengal Broth media. The secondary metabolite produced exogenously on broth media was extracted several times with ethyl acetate and concentrated by rotary evaporator. Screening was done via comparative study of crude extract and standard piperine under Thin Layer Chromatography (TLC) analysis. Piperine and some major derivatives were autodocked against active site of efflux protein Rv1258c of Mycobacterium tuberculosis. Comparative docking of piperine and some widely used NSAIDS with active site of COX2 enzymes was done to understand anti-inflammatory potential of piperine. Fungal endophytes identification was done on morphological basis by Lacto phenol Cotton Blue staining focusing on conidiophores and conidia. Crude extract from fungal fermentation and standard piperine showed similar TLC analysis and antimicrobial activity results. Auto-docking result showed piperitine to have least binding energy (-104.914kCal/Mole) with active site of efflux protein Rv1258c. Also, compared to widely used NSAIDs (Aspirin, Ibuprofen, Declofenac), piperine showed lesser binding energy (-92.383kCal/Mole) with active site of prostaglandin synthesizing enzyme COX2. Fungal fermentation mediated successful extraction of piperine in this study suggests for future approach for large-scale commercial production of such natural bioactive compounds. Piperine derivative piperitine can be successfully used in ant-tubercular therapy for enhancing bioavailability of antimycobacterial drug like rifampicin. The latent toxicity of anti-inflammatory drugs can be well addressed by use of natural bioactive compounds like piperine showing anti-inflammatory activity.


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