Degradation of feathers by bacterial consortium and its application in seed germination
- 1Department of Microbiology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Baramati, Maharashtra, India
- 2Department of Microbiology, H.V. Desai College, Pune, Maharashtra, India
Int. Res. J. Biological Sci., Volume 8, Issue (1), Pages 20-23, January,10 (2019)
Feathers primarily composed of keratin are largely produced as a waste by-product at poultry plants. Keratin is an insoluble protein macromolecule with very high stability and low degradation rate. Use of keratinolytic bacteria have found a promising effect in shortening the degradation time. In present study consortium of three organisms was used for feather degradation and the feather degraded product was used to check effect on seed germination. It was seen that the consortium was able to degrade 92.6% of feathers within 72 hours. The degraded product proved to be rich source of nitrogen. The application of feather degraded product in seed germination of Gram seeds had astounding results. The effect was measured in terms of root-shoot length, number of secondary roots, root shoot ratio, etc. The root: shoot ratio was increased in control group (2.7) compared with test (1.9) indicating nutrient deficiency. Thus, the feather degraded product could be used to increase seed germination rate.
- Vasileva-Tonkova E., Gousterova A. and Neshev G. (2009)., Ecologically safe method for improved feather wastes biodegradation., International Biodeterioration and Biodegradation, 63(8), 1008-1012.
- Brandelli A., Daroit D.J. and Riffel A. (2010)., Biochemical features of microbial keratinases and their production and applications., Applied Microbiology and Biotechnology, 85(6), 1735-1750.
- Deivasigamani B. and Alagappan K.M. (2008)., Industrial application of keratinase and soluble proteins from feather keratins., Journal of Environmental Biology, 29(6), 933-936.
- Papadopoulos M.C., El Boushy A.R., Roobdeen A.E. and Ketelaars E.H. (1986)., Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal., Anim. Feed Sci. Technol., 14(3-4), 279-290.
- Onifade A.A., Al-Sane N.A., Al-Musallam A.A. and Al-Zarban S. (1998)., A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources., Bioresource Technology, 66, 1-11.
- Selvam K. and Vishnupriya B. (2012)., Biochemical and molecular characterization of microbial keratinase and its Remarkable Applications., International Journal of Pharmaceutical and Biological Archieves, 3(2), 267-275.
- Choi J.M. and Nelson P.V. (1996)., Developing a slow-release nitrogen fertilizer from organic sources using poultry feathers., Journal of the American Society of Horticultural Science, 121(4), 634-638.
- Kim W.K., Lorenz E.S. and Patterson E.S. (2002)., Effect of Enzymatic and Chemical Treatments on Feather Solubility and Digestibility., Poult. Sci., 81, 95-98.
- Paul T., Halder S.K., Das A., Bera S., Maity C., Mandal A. and Mondal K.C. (2013)., Exploitation of chicken feather waste as a plant growth promoting agent using keratinase producing novel isolate Paenibacillus woosongensis TKB2., Biocatalysis and Agricultural Biotechnology, 2(1), 50-57.
- Anwar M.S., Siddique M.T., Verma A., Rao Y. R., Nailwal T., Ansari M. and Pande V. (2014)., Multitrait plant growth promoting (PGP) rhizobacterial isolates from Brassica juncea rhizosphere: keratin degradation and growth promotion., Communicative & integrative biology, 7(1), e27683.
- Kornillowicz-Kowalska T. and Bohacz J. (2011)., Biodegradation of keratin waste: Theory and practical aspects., Waste Management, 31(8), 1689-1701.
- Vasileva-Tonkova E., Gousterova A. and Neshev G. (2009)., Ecologically safe method for improved feather wastes biodegradation., International Biodeterioration & Biodegradation, 63(8), 1008-1012.
- Ichida J.M., Krizova L., LeFevre C.A., Keener H.M., Elwell D.L. and Burtt Jr, E.H. (2001)., Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost., Journal of Microbiological Methods, 47(2), 199-208.
- Maerere A.P., Kimbi G.G. and Nonga D.L.M. (2001)., Comparative effectiveness of animal manures on soil chemical properties, yield and root growth of amaranthus (Amaranthus cruentus L.)., African Journal of Science and Technology, 1(4), 14-21.
- Megan B. (2013)., Education.com, updated on Oct 25, 2013.