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Hydrocarbon degradation potential of some hydrocarbon-utilizing bacterial species associated with Kenaf (Hibiscus cannabinus L.) plant

Author Affiliations

  • 1Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigeria
  • 2Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigeria

Int. Res. J. Biological Sci., Volume 8, Issue (1), Pages 10-19, January,10 (2019)


13 Kenaf (Hibiscus cannabinus) plant associated Hydrocarbon-Utilizing Bacterial isolates previously identified based on their morphological, biochemical and molecular characteristics as Providencia vermicola_01, Providencia rettgeriB10_04, Providencia rettgeri D04_10,Pseudomonas aeruginosa D10_10, Exiguobacterium aurantiacum F03_18, Providencia vermicola F10_16, Klebsiella pneumoniae G10_19, Stenotrophomonas maltophila A04_01, Providencia rustigianii H10_22, Lysinibacillus fusiformis B11_05, Lysinibacillus sphaericus C11_08, Pseudomonas aeruginosa F04_16 and Lysinibacillus sphaericus E11_were investigated for their degradation potential in crude oil, diesel and engine oil. The Kenaf (Hibiscus cannabinus) plant was grown in a Niger Delta soil. Growth of these Kenaf associated bacteria was assessed for 7 days by monitoring the optical density (OD) of the media. OD values were observed to rise majorly between the first and three days of contact between the HUB and the hydrocarbons. The highest OD values measured for crude oil degradation was 1.548nm by Klebsiella pneumoniae G10_19, for engine oil 1.416nm by Klebsiella pneumonia and by Exiguobacterium aurantiacum 1.416nm. Significant difference in o.d. values was observed only for Exiguobacterium aurantiacum F03_18. This study provided information on suitable Kenaf (Hibiscus cannabinus) HUB bacterial species to use for phytoremediation of engine oil, crude oil and diesel impacted soil in Niger Delta Region.


  1. Vieira P.A., Vieira R.B., De França F.P. and Cardoso V.L. (2007)., Biodegradation of effluent contaminated with diesel fuel and gasoline., Journal of Hazardous Materials, 140(1-2), 52-59.
  2. Romantschuck M., Sarand I., Petänen T., Peltola R., Johnsson‐Vihanne M., Koivula T., Yrjala K. and Haahtela K. (2000)., Means to Improve the Effect of In situ Bioremediation of Contaminated Soil: An Overview of Novel Approaches., Envir. Pol, 107(2), 179‐185.
  3. Chaillan F., Le Flèche A., Bury E., Phantavong Y.H., Grimont P., Saliot A. and Oudot J. (2004)., Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms., Research in microbiology, 155(7), 587-595.
  4. Agwu M.O. (2013)., Community Participation and Sustainable Development in the Niger Delta., Brit. J. of Edu. Soc and Beha. Sci., 3, 33-46.
  5. Oviasuyi P.O. and Uwadiae J. (2010)., The Dilemma of Niger Delta Region as Oil Producing States of Nigeria., J. of P. Con. and. Dev, 16, 110-126.
  6. Olanrewaju J.S. (2015)., The Issues of Integration and Marginalization in a Federation: South-South Question in Nigerian Politics., Int. J. of Pol and G. Gov., 6, 1-16.
  7. Agbonifo P. (2016)., Oil Spills Injustices in the Niger Delta region: Reflections on Oil Industry Failure in Relation to the United Nations Environment Programme (UNEP) REPORT., Int J. of Pet and Gas. Exp. Mgt, 2, 26-37.
  8. Karthikeyan R. and Kulakow P.A. (2003)., Soil Plant Microbe Interactions in Phytoremediation., Adv. Bio. Eng. Bio, 78, 52-74.
  9. Okoh A.I. (2003)., Biodegradation of Bonny light Crude Oil in Soil Microcosm by some Bacterial Strains Isolated from Crude Oil Flow Stations Saver Pits in Nigeria., Afr. J. of Bio, 2(5), 104-108.
  10. Adeline S.Y., Ting C. and Aw C.S. (2009)., Hydrocarbon-degradation by isolate Pseudomonas lundensis UTAR FPE2., Malaysian Journal of Microbiology, 5(2), 104-108.
  11. Abu G.O. and Ogiji P.A. (1996)., Initial test of a bioremediation scheme for the cleanup of an oil-polluted waterbody in a rural community in Nigeria., Bioresource Technology, 58(1), 7-12.
  12. Chikere C.B. and Ekwuabu C.B. (2014)., Molecular characterization of autochthonous hydrocarbon utilizing bacteria in oil-polluted sites at Bodo Community, Ogoni land, Niger Delta, Nigeria., Nig. J. of Bio, 27, 28-33.
  13. Ikuesan F.A., Boboye B.E. and Adetuyi F.C. (2015)., Cow dung as inoculum carrier for the degradation of crude oil., Sky Journal of Microbiology Research, 3(4), 47-54.
  14. Jeswani H. and Mukherji S. (2013)., Batch Studies with Exiguobacterium aurantiacum Degrading Structurally Diverse Organic Compounds and its Potential For Treatment of Biomass Gasification Waste Water., Int. Bio. and Bio, 80, 1-9.
  15. Mohanty G. and Mukherji S. (2008)., Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia., International Biodeterioration & Biodegradation, 61(3), 240-250.
  16. Moneke A. and Nwangwu C. (2011)., Studies on the bioutilization of some petroleum hydrocarbons by single and mixed cultures of some bacterial species., African Journal of Microbiology Research, 5(12), 1457-1466.