Isolation of Indole acetic acid producing bacteria from digester effluent and their effect on plant growth promotion
- 1Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India
Int. Res. J. Biological Sci., Volume 8, Issue (1), Pages 1-9, January,10 (2019)
The synthesis and release of indole acetic acid is an important property of bacteria that play key role in stimulating growth of crops. Indole acetic acid is phytohormone involved in growth and development in plants. The study aimed for isolation and to identify IAA producing bacteria from digester effluent of vegetable waste based biomethanation plant and to test its growth stimulatory effect on crops. Seventeen bacteria were isolated from digester effluent. They were tested to determine production ability for IAA. Two most potent IAA producing bacteria were selected further to test its stimulatory effect on growth of crops by pot assay method. The potent IAA producing plant growth promoting bacterial isolates were identified by molecular characterization using 16 S rRNA gene analyses. The results obtained from pot experiment demonstrated that two most potent IAA producing bacterial isolates cause significant increase in plant development parameters of inoculated crop plants by comparing with control. The study suggests two potent IAA producing bacteria from digester effluent can serve as efficient biofertilizer inoculants to enhance soil fertility and plant growth promotion.
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