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Molecular phylogeny and authentication of selected meliaceae members based on chloroplast rbcL sequencing studies

Author Affiliations

  • 1Post Graduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Manonmaniam Sundaranar University, Abishekapatti 627 012, Tirunelveli, Tamil Nadu, India
  • 2Post Graduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Manonmaniam Sundaranar University, Abishekapatti 627 012, Tirunelveli, Tamil Nadu, India
  • 3Post Graduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Manonmaniam Sundaranar University, Abishekapatti 627 012, Tirunelveli, Tamil Nadu, India

Int. Res. J. Biological Sci., Volume 7, Issue (12), Pages 38-41, December,10 (2018)

Abstract

Plants are used as a source of medicine in Traditional Medicinal Systems in India from time immemorial. The demand in medicinal plants leads to the adulteration in pharmaceutical industries. Low grade materials replaced with original one and most of the medicinal plants are sold as root, powder, leaf, fruit or stem, it leads to problem in plant identification. In the present investigation molecular phylogenetic analysis and authentication of medicinal plants Cipadessa baccifera and Melia azedarach belonging to the family Meliaceace was carried out using rbcL sequencing studies. The rbcL sequencing of the selected plants analysed with BLAST for similar sequence studies and the alignment was done with T-coffee software. The phylogenetic relationship between the species was constructed by MEGA software. BLAST results indicated the number of similar sequences present in the Genbank repository. The phylogenetic tree represented the degree of similarity and variation with other species and other accessions of same species. The result of the study was useful for the authentication of selected meliaceae members. The phylogenetic tree showed their relationship with other meliaceae members.

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