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Prevalence of multi drug resistant salmonella sp from meat samples of Hyderabad city, India

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Author Affiliations

  • 1Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka-585106, India
  • 2Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka-585106, India
  • 3Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka-585106, India
  • 4Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka-585106, India
  • 5Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka-585106, India

Int. Res. J. Biological Sci., Volume 7, Issue (12), Pages 15-20, December,10 (2018)

Abstract

Food is important need of survival for humankind. Food infections and etiological agents associated food is a serious threat. In the current study involved with Salmonella sp isolation and screening from retail meat shops and evaluation of antibiotic susceptibility. A total of 287 samples meat (goat meat-147 and chicken meat-140) were collected from retail meat shops of Hyderabad city, India. Samples were processed as per the ISO 6579:2002 and Salmonella isolates were identified based on the standard biochemical and cultural characterization. The serotyping was carried out by Kauffmann-White scheme for species level identification. Evaluation of antibiotic susceptibility test to various antibiotics as per CLSI guidelines and MIC of Ceftazidime and Ciprofloxacin was determined. Out of 287, 56 samples were positive for Salmonella with an incidence rate of 19.57%. The highest incidence of 22% was recorded in chicken meat samples and the goat meat samples had incidence rate of 16.32% (n=24). Based on serotyping, 87.5% (n=49), and 12.5% (n=7) isolates were identified as S. typhimurium and S. enteridis. A total of 85.7 % (n=48) of the 56 Salmonella isolates are multidrug resistant (MDR) and subsequently, ES&

References

  1. Bhunia A.K. (2018)., Foodborne microbial pathogens: mechanisms and pathogenesis., Springer, 2008. Salmonella enteric, 201-215.
  2. Rahman H. (2002)., Some aspects of molecular epidemiology & charcterisation of Salmonella Typhimurim isolated from man & animals., Indian Journal of Medical Research, 115, 108.
  3. Nørrung B. and Buncic S. (2008)., Microbial safety of meat in the European Union., Meat Science, 78(1-2), 14-24.
  4. Ray B. (2004)., Fundamental food microbiology., CRC Press, Boca Raton, FL.
  5. Hernandez T., Sierra A., Rodriguez-Alvarez C., Torres A., Arevalo M.P., Calvo M. and Arias A. (2005)., Salmonella enterica serotypes isolated from imported frozen chicken meat in the Canary islands., Journal of food protection, 68(12), 2702-2706.
  6. Bouchrif B., Paglietti B., Murgia M., Piana A., Cohen N., Ennaji M.M. and Timinouni M. (2009)., Prevalence and antibiotic-resistance of Salmonella isolated from food in Morocco., The Journal of Infection in Developing Countries, 3(01), 35-40.
  7. Eblen D.R., Barlow K.E. and Naugle A.L. (2006)., US Food Safety and Inspection Service testing for Salmonella in selected raw meat and poultry products in the United States, 1998 through 2003: an establishment-level analysis., Journal of food protection, 69(11), 2600-2606.
  8. Jordan E., Egan J., Dullea C., Ward J., McGillicuddy K., Murray G. and McDowell S. (2006)., Salmonella surveillance in raw and cooked meat and meat products in the Republic of Ireland from 2002 to 2004., International journal of food microbiology, 112(1), 66-70.
  9. Kaye K.S., Engemann J.J., Fraimow H.S. and Abrutyn E. (2004)., Pathogens resistant to antimicrobial agents: epidemiology, molecular mechanisms, and clinical management., Infectious disease clinics of North America, 18(3), 467-511.
  10. Arslan S. and Eyi A. (2010)., Occurrence and antimicrobial resistance profiles of Salmonella species in retail meat products., Journal of food protection, 73(9), 1613-1617.
  11. Straley B.A., Donaldson S.C., Hedge N.V., Sawant A.A., Srinivasan V., Oliver S.P. and Jayarao B.M. (2006)., Public health significance of antimicrobial-resistant gram-negative bacteria in raw bulk tank milk., Foodbourne Pathogens & Disease, 3(3), 222-233.
  12. White D.G., Zhao S., Sudler R., Ayers S., Friedman S., Chen S. and Meng J. (2001)., The isolation of antibiotic-resistant Salmonella from retail ground meats., New England journal of medicine, 345(16), 1147-1154.
  13. Livermore D.M. (1995)., beta-Lactamases in laboratory and clinical resistance., Clinical microbiology reviews, 8(4), 557-584.
  14. Paterson D.L. (2006)., Resistance in gram-negative bacteria: Enterobacteriaceae., American journal of infection control, 34(5), S20-S28.
  15. Andrews W.H., Andrew J. and Hammack T. (2011)., Salmonella. In: Bacteriological Analytical Manual., Federal Drug Administration, 8th ed., Revision A, Ch. 5. USFDA.
  16. Clarke P.H. and Cowan S.T. (1952)., Biochemical methods for bacteriology., Microbiology, 6(1-2), 187-197.
  17. Kauffmann F. (1972)., Serological diagnosis of Salmonella species., Mungksgaard, Copenhagen.
  18. Clinical and Laboratory Standards Institute (2012)., Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard., 11th ed. Clinical and Laboratory Standards Institute, Wayne.
  19. Andrews J.M. (2001)., Determination of minimum inhibitory concentrations., Journal of antimicrobial Chemotherapy, 48(1), 5-16.
  20. Ellermeier C.D. and Slauch J.M. (2006)., The genus Salmonella., In The prokaryotes, Springer, New York, NY., 123-158.
  21. Ray B. (2004)., Fundamental Food Microbiology., CRC Press, Boca Raton. New York, 225-238.
  22. Popoff M.Y. and Le Minor L.E. (2005)., Bergey’s Manual of Systematic Bacteriology., Wiliam and wikins Bakimore, P545, 2, 198.
  23. Van T.T.H., Moutafis G., Istivan T., Tran L.T. and Coloe P.J. (2007)., Detection of Salmonella spp. in retail raw food samples from Vietnam and characterization of their antibiotic resistance., Applied and Environmental Microbiology, 73(21), 6885-6890.
  24. Phan T.T., Khai L.T., Ogasawara N., Tam N.T., Okatani A.T., Akiba M. and Hayashidani H. (2005)., Contamination of Salmonella in retail meats and shrimps in the Mekong delta, Vietnam., Journal of Food Protection, 68(5), 1077-1080.
  25. Vindigni S.M., Srijan A., Wongstitwilairoong B., Marcus R., Meek J., Riley P.L. and Mason C. (2007)., Prevalence of foodborne microorganisms in retail foods in Thailand., Foodborne Pathogens and Disease, 4(2), 208-215.
  26. Donado-Godoy P., Clavijo V., Leon M., Tafur M.A., Gonzales S., Hume M. and Doyle M.P. (2012)., Prevalence of Salmonella on retail broiler chicken meat carcasses in Colombia., Journal of food protection, 75(6), 1134-1138.
  27. Dong P., Zhu L., Mao Y. and Liang R. (2014)., Prevalence and profile of Salmonella from samples along the production line in Chinese beef processing plants., Food Control, 38, 54-60.
  28. Little C.L., Richardson J.F., Owen R.J., De Pinna E. and Threlfall E.J. (2008)., Campylobacter and Salmonella in raw red meats in the United Kingdom: prevalence, characterization and antimicrobial resistance pattern, 2003-2005., Food microbiology, 25(3), 538-543.
  29. Bachhil V.N. and Jaiswal T.N. (1988)., Occurrence of Salmonella in meats., J. Food Sci. Technol., 25(5), 310-312.
  30. Selvaraj R., Das R., Ganguly S., Ganguli M., Dhanalakshmi S. and Mukhopadhayay S.K. (2010)., Characterization and antibiogram of Salmonella spp. From poultry specimens., J. Microbiol. Antimicrob., 2, 123-126.
  31. Mayrhofer S., Paulsen P., Smulders F.J. and Hilbert F. (2004)., Antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry., International Journal of Food Microbiology, 97(1), 23-29.
  32. White D.G., Zhao S., Simjee S., Wagner D.D. and McDermott P.F. (2002)., Antimicrobial resistance of foodborne pathogens., Microbes and infection, 4(4), 405-412.