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Antagonistic effect of bacteriocin-like substances against biofilms formed by P. aeruginosa, K. pneumonia, and P. vulgaris

Author Affiliations

  • 1Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
  • 2Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
  • 3Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
  • 4Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh

Int. Res. J. Biological Sci., Volume 7, Issue (1), Pages 1-5, January,10 (2018)

Abstract

Biofilms are frequently associated with many diseases including urinary tract infections. Structural organization and chemical properties of these assorted growths of microorganisms confer them the ability to resist antimicrobial therapy and thus infections caused by these microorganisms are becoming increasingly difficult to contain. Urinary catheters are typically involved in urinary tract infections due to their susceptibility to biofilm formation. Therefore, inhibition and/ or reduction of biofilms is critical to reduce catheter-associated infections. In this study, we used bacteriocin like substances produced by Lactobacillus coryniformis and Lactobacillus casei to reduce and/ inhibit biofilms formed by several pathogenic bacteria that are frequently associated with urinary tract infections. We isolated and identified eleven biofilm-forming bacterial isolates from urinary catheters collected from hospitalized patients: five Pseudomonas aeruginosa, three Klebsiella pneumoniae, and three Proteus vulgaris. Biofilm-forming capacity of the isolates was examined using microtitre plate method and three isolates showing the strongest biofilm forming capacity were selected for further investigation. To determine the effects of bacteriocin like substances, culture free supernatants (CSF) were prepared from Lactobacillus casei and Lactobacillus coryniformis and partially purified to remove non-proteinaceous substances. The CFS and partially purified substances (PPPs) were examined against biofilms formed by the bacterial isolates. Both the CFS and PPPs reduced biofilms formed by the isolates tested in our study. Our results indicate that biofilms formed by the P. aeruginosa, K. pneumoniae, and P. vulgaris isolates were significantly reduced upon treatment with bacteriocin like substances from the lactobacilli species used in our study.

References

  1. Donlan R.M. (2002)., Biofilms: microbial life on surfaces., Emerging infectious diseases, 8(9), 881-890.
  2. Saint S. and Chenoweth C.E. (2003)., Biofilms and catheter-associated urinary tract infections., Infect Dis Clin North Am, 17(2), 411-432.
  3. Weber D.J., Sickbert-Bennett E.E., Gould C.V., Brown V. M., Huslage K. and Rutala W.A. (2011)., Incidence of catheter-associated and non-catheter-associated urinary tract infections in a healthcare system., Infection control and hospital epidemiology, 32(8), 822-823.
  4. Singha P., Locklin J. and Handa H. (2017)., A review of the recent advances in antimicrobial coatings for urinary catheters., Acta biomaterialia, 50, 20-40.
  5. Emori T.G. and Gaynes R.P. (1993)., An overview of nosocomial infections, including the role of the microbiology laboratory., Clinical microbiology reviews, 6(4), 428-442.
  6. Trautner B.W. and Darouiche R.O. (2004)., Role of biofilm in catheter-associated urinary tract infection., American journal of infection control, 32(3), 177-183.
  7. Pollard J., Wright J., Feng Y., Geng D., Genberg C. and Savage P.B. (2009)., Activities of ceragenin CSA-13 against established biofilms in an in vitro model of catheter decolonization., Anti-Infective Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Infective Agents), 8(4), 290-294.
  8. Parada J.L., Caron C.R., Medeiros A.B.P. and Soccol C.R. (2007)., Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives., Brazilian Archives of Biology and Technology, 50(3), 512-542.
  9. Heinemann C., van Hylckama Vlieg J.E., Janssen D.B., Busscher H.J., van der Mei H.C. and Reid G. (2000)., Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131., FEMS microbiology letters, 190, 177-180.
  10. Stepanovic S., Vukovic D., Hola V., Di Bonaventura G., Djukic S., Cirkovic I. and Ruzicka F. (2007)., Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci., APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 115, 891-899.
  11. Dusane D.H., Damare S.R., Nancharaiah Y.V., Ramaiah N., Venugopalan V.P., Kumar A.R. and Zinjarde S.S. (2013)., Disruption of microbial biofilms by an extracellular protein isolated from epibiotic tropical marine strain of Bacillus licheniformis., PloS one, 8, e64501.
  12. Breukink E. (2006)., A lesson in efficient killing from two-component lantibiotics., Molecular microbiology, 61(2), 271-273.
  13. Dusane D.H., Pawar V.S., Nancharaiah Y.V., Venugopalan V.P., Kumar A.R. and Zinjarde S.S. (2011)., Anti-biofilm potential of a glycolipid surfactant produced by a tropical marine strain of Serratia marcescens., Biofouling, 27(6), 645-654.
  14. Dusane D.H., Rajput J.K., Kumar A.R., Nancharaiah Y.V., Venugopalan V.P. and Zinjarde S.S. (2008)., Disruption of fungal and bacterial biofilms by lauroyl glucose., Letters in applied microbiology, 47(5), 374-379.
  15. Kwiecinska-Piróg J., Bogiel T., Skowron K., Wieckowska E. and Gospodarek E. (2014)., Proteus mirabilis biofilm-Qualitative and quantitative colorimetric methods-based evaluation., Brazilian Journal of Microbiology, 45(4), 1423-1431.
  16. Al-Mathkhury H.J., Ali A.S. and Ghafil J.A. (2011)., Antagonistic effect of bacteriocin against urinary catheter associated Pseudomonas aeruginosa biofilm., North American journal of medical sciences, 3(8), 367-370.
  17. Macleod S.M. and Stickler D.J. (2007)., Species interactions in mixed-community crystalline biofilms on urinary catheters., Journal of medical microbiology, 56(11), 1549-1557.
  18. Lü X., Yi L., Dang J., Dang Y. and Liu B. (2014)., Purification of novel bacteriocin produced by Lactobacillus coryniformis MXJ 32 for inhibiting bacterial foodborne pathogens including antibiotic-resistant microorganisms., Food Control, 46, 264-271.
  19. Lü X., Hu P., Dang Y. and Liu B. (2014)., Purification and partial characterization of a novel bacteriocin produced by Lactobacillus casei TN-2 isolated from fermented camel milk (Shubat) of Xinjiang Uygur Autonomous region, China., Food control, 43, 276-283.