7th International Science Congress (ISC-2017).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

In-vivo study of stress oxidative and liver damage in rats exposed to acetate lead

Author Affiliations

  • 1Department of Cellular and Molecular Biology, Faculty of natural sciences and life, ElOued University, El-Oued 39000, El-Oued, Algeria
  • 2Department of Cellular and Molecular Biology, Faculty of natural sciences and life, ElOued University, El-Oued 39000, El-Oued, Algeria
  • 3Department of Cellular and Molecular Biology, Faculty of natural sciences and life, ElOued University, El-Oued 39000, El-Oued, Algeria
  • 4Department of Cellular and Molecular Biology, Faculty of natural sciences and life, ElOued University, El-Oued 39000, El-Oued, Algeria

Int. Res. J. Biological Sci., Volume 6, Issue (9), Pages 1-6, September,10 (2017)

Abstract

Lead (Pb) is a very toxic divalent heavy metal that occurs and diffuses into nature and the environment through human activities. The aim of this study was to evaluate the structure and liver function in rats exposed to lead. Our experimentation is carried out on Ten female Wistar rats were divided into two groups (n=5). The first group of rats received normal diet and water (controls) and the second group of rats received normal diet and acetate lead-contaminated water (100 mg/kg b.w) for 70 days. On which we measured some biochemical parameters. Results of our study showed that, in comparison with the control rats, lead exposure caused, a significant reduction (p<0.001) in the body weight and a significant augmentation (p<0.001) in relative liver weight. In addition, Result showed that in Pb-intoxicated rats, an increase in serum transaminases and Alkaline phosphatase activities and a decrease of GOT and GPT activities in liver when compared with normal animal group. Results revealed also that acetate lead treatment in rats affected antioxidant defense system by decreasing GSH level and GST activity and increasing MDA concentration. Also, the results clearly showed that lead causes alterations of hepatic tissue in comparison with controls. In Conclusion, Results demonstrated the toxic effect of high-dose of lead by causing oxidative stress and damage in hepatic tissue.

References

  1. Derouiche, S. and Djouadi, A. (2017). Control of Lead and Cadmium in Cosmetic Product (Kohl) of Pits Dates by Cyclic Voltammetry. J. Chem. Pharm. Res., 9(3),319-323, undefined, undefined
  2. Adhikari N., Sinha N. and Narayan R. (2001)., Lead-Induced Cell Death in Testes of Young Rats., J. Appl. Toxicol., 21(4), 275-277.
  3. Bukola R.O., Adeleke A.A., Omamuyovwi M.I. and Stephen O.A. (2015)., Lead- Induced Damage on Hepatocytes and Hepatic Reticular Fibres in Rats; Protective Role of Aqueous Extract of Moringaoleifera Leaves (Lam)., J Biosci Med., 3, 27-35.
  4. Simkhovich B.Z., Kleinman T.M. and Kloner R.A. (2008)., Air pollution and cardiovascular injury: Epidemiology, Toxicology, and Mechanisms., J. Am. Coll. Cardiol., 52(9), 719-726.
  5. Sharma A., Sharma Veena and Kansal Leena (2010)., Amelioration of lead-induced hepatotoxicity by Allium sativum extracts in Swiss albino mice., Libyan J. Med., 5, 1-10.
  6. Santhosh R.K., Arul J.D., Annie S.J. and Asha S.D. (2014)., HepatoprotectiveRole of Abutilon indicum on Lead Induced Liver Injury in Wistar Rats., Int. J. Pharm. Sci. Rev. Res., 40(2), 36-39.
  7. Flora S.J.S, Mittal M. and Mehta A. (2008)., Heavy metal induced oxidative stress and its possible reversal by chelation therapy., Indian J Med Res., 128, 501-523.
  8. Kansal Leena, Sharma Veena, Sharma Arti, Lodi Shweta and Sharma S.H. (2011)., Protective role of coriandrum sativum (coriander) extracts against lead nitrate induced oxidative stress and tissue damage in the liver and kidney in male mice., Int. J. Appl. Biol. Pharm., 2(3), 65-83.
  9. Derouiche S. and Kechrid Z. (2016)., Zinc Supplementation Overcomes Effects of Copper on Zinc Status, Carbohydrate Metabolism and Some Enzyme Activities in Diabetic and Nondiabetic Rats., Can. J. Diabetes, 40(4), 342-347.
  10. Djouadi Anfal and Derouiche Samir (2017)., Study of fluoride-induced haematological alterations and liver oxidative stress in rats., World J Pharm sci., 6(5), 211-221.
  11. Sastre J., Pallardo F.V., Asuncion J. and Vina J. (2000)., Mitochondria, oxidative stress and aging., Free. Radic. Res., 32(3), 189-198.
  12. Derouiche Samir, Manel Djermoune and Kawther Abbas (2017)., Beneficial Effect of Zinc on diabetes induced kidney damage and liver stress oxidative in rats., J. adv. biol., 10(1), 2050-2055.
  13. Habig W.H., Pabst M.J. and Jakoby W.B. (1974)., Glutathione S-transferase. The first enzymatic step in mercapturic acid formation., J. Biol. Chem., 249(22), 7130-7139.
  14. Reckziegel P., Dias V.T., Benvegnú D.M., Boufleur N., Barcelos R.C., Segat H.J., Pase C.S., Santos C.M. and Bürger M.E. (2016)., Antioxidant protection of gallic acid against toxicity induced by Pb in blood, liver and kidney of rats., Toxicol Rep., 3, 351-356.
  15. Zaheer A., Iqbal M.Z. and Shoro A.A. (2013)., Lead-Induced Reduction in Body and Kidney Weight of Wistar Albino Rats Ameliorated by Ginkgo biloba Extract (EGb 761)., Biochem. Physiol., 2(2), 1-4.
  16. Ahmed Marwa A. and Hassanein Khaled M.A. (2013)., Cardio protective effects of Nigella sativa oil on lead induced cardio toxicity: Anti-inflammatory and antioxidant mechanism., J. Physiol. Pathophysiol., 4(5), 72-80.
  17. Kilikdar D., Mukherjee D., Mitra E., Ghosh A.K., Basu A., Chandra M.A. and Bandyoapdhyay D. (2011)., Protective effete of aqueous galic extract against lead-induced hepatic injury in rats., Indian J. Exp. Biol., 49, 498-510.
  18. Mudipalli A. (2007)., Lead hepatotoxicity and potential health effects., Indian J. Med. Res., 126(6), 518-527.
  19. Derouiche S., Azzi M. and Hamida A. (2017)., Effect of extracts aqueous of phragmites australis on carbohydrate metabolism, some enzyme activities and pancreatic islet tissue in alloxaninduced diabetic rats., Int. J. Pharm. Pharm. Sci., 9(6), 54-58.
  20. Jomova K. and Valko M. (2011)., Advances in metal-induced oxidative stress and humandisease., Toxicology., 283, 65-87.
  21. Derouiche Samir and Kechrid Zine (2013)., Influence of calcium supplements on zinc status, carbohydrate metabolism and the liver activity of detoxifying glutathione enzymatic system in alloxan-induced diabetic rats., J. Exp. Biol.Agr. Sci., 1(6), 424-429.
  22. Attia A.M.M, Ibrahim A.A.F, Ghada M.N. and Aziz W.S. (2013)., Antioxidant effects ofginger (Zingiberofficinale Roscoe) against lead acetate-induced hepatotoxicity in rats., Afr. J. Pharm. Pharmacol., 7(20), 1213-1219.
  23. Reckziegel P., Dias V.T., Benvegnú D.M., Boufleur N., Silva Barcelos R.C., Segat H.J, Pase C.S., Santos C.M. and Bürger M.E. (2016)., Antioxidant protection of gallic acid against toxicity induced by Pb in blood, liver and kidney of rats., Toxicology rep., 3, 351-356.
  24. Derouiche S. and Djouadi A. (2017)., An evaluation of stress oxidative and serum electrolytes in female hypothyroid patients., Int. J. Biol. Med. Res., 8(1), 5861-5865.
  25. Sarkar S., Mukherjee S., Chattopadhyay A. and Bhattachary S. (2014)., Low dose of arsenic trioxide triggers oxidative stress in zebrafish heart: Expression of antioxidant genes., Ecotoxicol. Environ. Saf., 107, 1-8.
  26. Bas H., Kalender S., Karaboduk H. and Apaydin F.G. (2015)., The Effects on Antioxidant Enzyme Systems in Rat Heart Tissues of Lead Nitrate and Mercury Chloride., GU J Sci., 28(2), 169-174.
  27. Shalan M.G., Mostafa M.S., Hassouna M.M., Hassab S.E. and El-Nabi A.E. (2005)., Amelioration of lead toxicity on rat liver with vitamin C and Silymarin supplements., Toxicology, 206(1), 1-15.
  28. Aziz F.M. (2012)., Protective Effects of Latex of Ficuscarica L. against Lead Acetate-Induced Hepatotoxicity in Rats., Jordan J. Biol. Sci., 5(3), 175-182.