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Extraction, purification and characterization of pyocyanin produced by Pseudomonas aeruginosa and evaluation for its antimicrobial activity

Author Affiliations

  • 1Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh
  • 2Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh
  • 3Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh
  • 4Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh
  • 5Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh

Int. Res. J. Biological Sci., Volume 6, Issue (5), Pages 1-9, May,10 (2017)


Pseudomonas aeruginosa, a gram negative bacteria, which exerts broad antagonistic activity against other bacterial and fungal pathogens through the production of a secondary metabolite-pyocyanin. In the present study, various clinical samples were taken for P. aeruginosa isolation. From sixteen primary P. aeruginosa isolates, five isolates (PS1, PU5, PU8, PU10 and PP3) were selected, on the basis of pigmentation in cetrimide agar. The screening for antimicrobial activity of P aeruginosain cross streak method showed that Staphylococcus aureus (ATCC 6538), Salmonella enterica (NCTC 6017), and Bacillus cereus (ATCC 14579) were sensitive, Escherichia coli (ATCC 8739) was intermediate, and K. pneumoniae (ATCC 43816) was resistant to the inhibitory action of the selected P. aeruginosa isolates. The antimicrobial pigment pyocyanin was extracted from culture broth following solvent extraction method. Purification of the pigment was done by column and thin layer chromatography. The Rf value was found around 0.81 for all the extracted pigment solution. Confirmation of the pigment as pyocyanin was done through FTIR, and UV-visible spectrophotometric analysis. FTIR analysis revealed different functional groups (-OH, -C=N, -CH3 etc.) which belongs to the aromatic structure of pyocyanin. In UV-Vis spectrophotometric analysis, a maximum absorption was observed at 270-275nm.The modification of media composition enabled to increase pyocyanin production and the highest amount was produced by the isolate PU10 in Medium-B having a concentration of 9.45 &


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