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Combined effects of metals and chlorophenols on dehydrogenase activity of bacterial consortium

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Author Affiliations

  • 1Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 2Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 3Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 4Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria

Int. Res. J. Biological Sci., Volume 6, Issue (4), Pages 10-20, April,10 (2017)

Abstract

Toxicity of Zinc, Cadmium, 4-Chlorophenol (4-CP), 2,4-Dichlorophenol (2,4-DCP) and their binary and quaternary mixtures were determined based on inhibition of dehydrogenase activity of a consortium of Pseudomonas, Bacillus, Micrococcus and Staphylococcus species. The toxicity of chemicals and their mixtures were evaluated in the concentration range of 0-3mM while Cadmium and 2,4-Dichlorophenol binary mixture range was 0-1.8mM. Zinc, 4-CP and 2,4-DCP exhibited hormetic effect at low concentration. The IC50 were determined using monotonic and hormesis dose-response models. The binary and quaternary mixtures of the pollutants evaluated showed progressive inhibition of the enzyme activity. The combined effects of the mixtures on the enzyme activity of the bacterial consortium were evaluated with isobolographic representation and toxic index (TI) model. The isobolographic analysis indicated additive, synergistic and antagonistic interactions for the various binary mixtures evaluated. However, the TI of most mixtures was within the range of 0.5-2.0 and are considered additive. Modulation of the toxic interactions by the components of the mixture through synergistic and antagonistic interaction of the heavy metals and phenolic compounds against the dehydrogenase activity of the bacterial consortium were possible depending on the relative amount of the components.

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