Use of leaves of mulberry, Morus alba (L) treated with Stevia Inulin for the improvement of activities of enzymes in the mid gut protease and amylase of the last stage silkworm larvae
- 1Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Tal-Baramati, Dist-Pune, Pin-413 115, India
- 2Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Tal-Baramati, Dist-Pune, Pin-413 115, India
Int. Res. J. Biological Sci., Volume 6, Issue (11), Pages 24-30, November,10 (2017)
The inulin is a heterogeneous collection of fructose polymers anda soluble dietary fiber. The present attempt is concerned with use of leaves of mulberry, Morus alba (L) treated with Stevia Inulin for the improvement of activities of mid gut protease and amylase in last stage silkworm larvae. Four different concentrations of aqueous solution of herbal formulation: Stevia inulin powder (5.0 mg/ lit; 10.0 mg/ lit; 20.0 mg/ lit and 50.0 mg/ lit) were used to soak the leaves of mulberry. The treated leaves were used feeding. The schedule of feeding for each strength of solution for the first group (A-First; B-First; C-First and D-First) was for the three days (First; Second and Third); For second group (A-Second; B-Second; C-Second and D-Second)) was for two days (second and third) and for the third group (A-Third; B-Third; C-Third and D-Third) was only for one day (third). The analysis of biochemical parameters was carried through the use of fifth stage instars of five days old. The biochemical characters considered for analysis were proteins (Soluble fraction of Proteins and Total fraction of Proteins). The activities of enzymes, namely protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) in were also considered in the present attempt. The tissue used for the biochemical analysis was mid gut homogenate. Soaking the mulberry leaves with Stevia inulin powder and supplying the treated leaves to last larval instar was found effected into significant changes contents of both soluble and total proteins. The activities of protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) were also found effected through the inulin treatment. The contents of soluble proteins and total proteins of mid gut homogenate pattern was found increased respectively from 32.147 percent to 90.074 percent and 5.657 percent to 39.052 percent. The activities of protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) were observed to increase from 21.444 to 83.706 and from 14.540 to 54.257 percent respectively. The composition of Stevia inulin powder serve to fortify the digestion. It may also exert the effects on activity in metabolism in the last stage larval of silkworm, Bombyx mori (L). Inclusion of the method of “Treating the leaves of mulberry with Stevia inulin powder before the feeding” may be effecting to speed up the rate of metabolism in the last larval instars of silkworm. Utilization of Stevia inulin powder for soaking the leaves of mulberry before supplying to the last larval stage of silkworm, Bombyx mori (L) may be considered for introduction in the “Methodlogy of Sericulture. It may help to improve the health of silkworm.
- Bowers W.S., Fales V.M., Thompson M.J. and Uebel B. (1966)., Juvenile and gonadotropic activity of 10, 11 epoxyfranesoic acid methyl ester., Life Science, 4(23), 2323-2331.
- Murugan K. and George A. (1992)., Feedings and nutritional influence on growth and reproduction of Daphnia near (L)., Insect Physiol., 38(12), 961-967.
- Ito T. (1960)., Effect of sugars on feeding the larvae of silkworm, Bombyx mori (L)., J. Insect. Physiol., 5(2), 95-107.
- Ito T. (1961)., Nutrition of silkworm., Bombyx mori (L). Proc. Jpn. Acad. Sci., 43, 57-61.
- Nayar J.K. and Frankel G. (1962). Journal of Insect Physiology, 8, 505., undefined, undefined
- Ito T., Kwashima K., Nakhara M., Nakanshi K. and Terahara A. (1964)., Metabolism in the mid gut of silkworm, Bombyx mori (L)., Insect Physiol., 10, 225-228.
- Murali K. (1992)., Effect of leaf carbohydrate reserves on the growthand excretory pattern of silkworm, Bombyx mori (L)., M. Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
- Subramanyam Reddy C. (1992)., Studies on distribution of digestive enzymes in the digestive tract of silkworm, Bomby xmori (L)., M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
- Slansky F. (1985)., Food consumption and Utilization. In: comprehensive Insect physiology, Biochemistry and pharmacology., (Eds. Kerkut, G.A. and Gilbert, L.I.) Pergamon Press, Oxford, 4, 87-163.
- Horie Y., Tanaka M. and Ito T. (1963)., Proteolytic enzyme of digestive juice of mid gut in silkworm, Bombyx mori (L)., J. Setricult. Sci. Japan, 32, 8-15.
- Yamafuji I. and Yonezawa (1935)., Lipases in silkworm, Bomby xmori (L)., Insect. Biochem, 1, 102-112.
- Khyadeand Vitthalrao B. and Kulkarni Jyoti A. (2011)., Effect of digoxin treated mulberry leaves on protein profiles in fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR2)., Res. J. Chem. Sci., 1(1), 2-6. (www.isca.in)
- Desai V.A., Pawar V.V. and Sawant R.T. (2011)., Influence of herbal drug: kho-go on the fifth instar larvae of silkworm, Bombyx mori (L)., Dissertation in the partial fulfillment of M.Sc. (Microbiology), Shardabai Pawar Mahila College, Shardanagar (Baramati), (Pune University, Pune).
- Bridel M. and Lavieille R. (1931)., The sweet principle in Kaa-he-e (Stevia rebaudiana. Bertoni). II. Hydrolysis of stevioside by enzymes. III. Steviol by enzymic hydrolysis and isosteviol by acid hydrolysis., Bulletin de la Societe de Chimie Biologique, 13, 781-796.
- Dolder Fred, Lichti Heinz, Mosettig Erich and Quitt Peter (1960)., The structure and stereochemistry of steviol and isosteviol., Journal of the American Chemical Society, 82, 246-247. doi:10.1021/ja01486a054.
- Krishnaswami S., Narasimhna M.N., Suryanarayana S.K. and Kumararaj S. (1978)., Sericulture Manual-II Silkworm Rearing: FAO., UnitedNations Rome.
- Khyade V.B. (2004)., Influence of juvenoids on silkworm, Bombyx mori (L)., Ph.D. thesis, Shivaji University, Kolhapur, Zoology Department.
- Lowery O.H., Rosenbrough N.J., Far A.L. and Randall R.J. (1951)., Protein measurement with folin phenol reagent., J. Biol. Chem., 193, 265-275.
- Brik Y., Harpaz J., Ishaya I. and Bhondi A. (1962)., Studies on proteolytic activity of beetle Tenebrio and Tribolium., J. Insect Physiol., 8(4), 417-429.
- Ishaaya I., Moore I. and Joseph B. (1971)., Protease and amylase activity in the larvae of Egyption cotton worm, Spodopteralittoralis (L). J. Insect physiol., 17(5), 945-953., undefined
- Chougale A.C. (1992)., Influence of magnetic energy on silkworm., Bombyxmori (L), Ph.D. Thesis, Shivaji University, Kolhapur.
- Bernfeld P. (1955)., Amylase, a and b., Methods of Enzymology, (Ed. Clockwikand Kalpin). Academic Press, New York, 1, 149-158.
- Gaikwad A.R. (1998)., Biology of some dung beetles of South Western Maharashtra. Ph.D. thesis., Shivaji University, Kolhapur.
- Baily Norman T.J. (1955)., Statistical Methods., 2nd ed., Halsted Press John Wileyand Co., New York, 216.
- Applebaum S.W. (1985)., Biochemistry of digestion., Comprehensive insect physiology, biochemistry and pharmacology, 4, 279-311.
- Khyade Vitthalrao and Sarawade Jeevan P. (2009)., Protein profiles in the fifth instar larvae of silkworm, Bombyx mori (L) (PM xCSR2) fed with digoxin treated mulberry leaves., The Bioscan, 4(1), 41-44.