7th International Science Congress (ISC-2017).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Contribution of phosphate solubilizing activity to plants by Pseudomonas sp. having antifungal activity

Author Affiliations

  • 1Biotechnology Research Department, Ministry of Education, Myanmar
  • 2Biotechnology Research Department, Ministry of Education, Myanmar
  • 3Biotechnology Research Department, Ministry of Education, Myanmar
  • 4Biotechnology Research Department, Ministry of Education, Myanmar
  • 5Biotechnology Research Department, Ministry of Education, Myanmar

Int. Res. J. Biological Sci., Volume 6, Issue (10), Pages 1-7, October,10 (2017)

Abstract

The application of chemical fungicides has caused health hazards in animals and humans due to their residual toxicity. The objective of this research work is to find antagonistic bacteria having plant growth promoting activity for the control of some plant pathogenic fungi. Isolation of antagonistic bacteria from rhizosphere of rice, colonial and microscopic morphology and biochemical characterization were done. For antagonistic activity, dual culture and well diffusion method were employed. P-solubilizing activity was detected by plate screening and Vogel method. Isolated bacteria were assumed as Pseudomonas sp. according to its characteristic. This bacteria exhibited antagonistic activity against eight plant pathogenic fungi. The highest activity gave against fungi infected on green gram (26 mm) by dual culture method and against Fusarium sp. in giving 21 mm of inhibition zone by well diffusion method. 264.20 ppm and 235.77 ppm of soluble phosphate was detected by this isolate. Isolated bacteria exhibited antagonistic activity against eight plant pathogenic fungi by dual culture and well diffusion methods. Its activity was higher on PDA media than on nutrient media. In addition, isolated bacteria also possess P-solubilizing activity.

References

  1. Spadaro D. and Gullino M.L. (2004)., State of the art and future prospects of the biological control of postharvest fruit diseases., Int J Food Microbio, 91(2), 185-194.
  2. Whipps J.M. (2001)., Microbial interactions and biocontrol in the rhizosphere., Jou of Experi Bot, 52, 487-511.
  3. Dev Neha and Dawande A.Y. (2010)., Biocontrol of soil borne plant pathogen Rhiozoctonia solani using Trichoderma spp. and Pseudomonas fluorescens., Asiatic J Biotech Res., 01, 39-44.
  4. Holmes G.J. and Eckert J.W. (1999)., Sensitivity of Penicillium digitatum and P.italicum to postharvest citrus fungicides in California., Phytopatho, 89, 716-721.
  5. Kinay P., Mansour M.F., Gabler F.M., Margosan D.A. and Smilanick J.L. (2007)., Characterization of fungicide-resistant isolates of Penicillium digitatum collected in California., Crop Prot, 26(4), 647-656.
  6. Deacon J.W. (1991)., Significance of ecology in the development of biocontrol agents against soil-borneplant pathogens., J of Biocon Sci and Tech, 1(1), 5-20.
  7. Compant S., Duffy B., Nowak J., Clément C. and Barka E.A. (2005)., Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects., Appli and Envi Micro., 71(9), 4951- 4959.
  8. Fernando W.G.D., Ramarathnam R., Krishnamoorthy A.S. and Savchuk S.C. (2005)., Identification and use of potential bacterial organic antifungal volatiles in biocontrol., Soil Biol Biochem., 37, 955-964.
  9. Whipps J.M. (1997)., Development in the biological control of soil-borne plant pathogens., Advan in Bota Res., 26, 1-134.
  10. Ryder M.H., Stephens P.M. and Bowen G.D. (1994)., Improving plant productivity with rhizosphere bacteria., In: Glen Osmond, SA: CSIRO Division of Soils, refs, illus, index, 288.
  11. Srivastava R. and Shalini (2008)., Antifungal activity of Pseudomonas fluorescens against different plant pathogenic fungi., The Inter J of Micro., 7(2), 2789-2796.
  12. Sivan A. and Chet I. (1989)., Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum., J of Gen Micro, 135, 675-682.
  13. McKeen C.D., Reilly C.C. and Pusey P.L. (1986)., Production and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis., Phytopatho, 76, 136-139.
  14. Chet I. (1988)., Mycoparasitism and lytic enzymes., 153-171. In G. E. Harman and C. P. Kubicek (ed.), Trichoderma and Gliocladium, 2. Enzymes, biological control and commercial appli- cation. Taylor and Francis Ltd., London, United Kingdom.
  15. Fiddaman P.J. and Rossall S. (1993)., The production of antifungal volatiles by Bacillus subtilis., J of Appli Bacterio, 74(2), 119-126.
  16. Chernin L. and Chet I. (2002)., Microbial enzymes in biocontrol of plant pathogens and pests., Enzymes in the Environment: Activity, Ecology, and Applications. (R Burns and R Dick eds.), Marcel Dekker, Inc., New York, 171-225.
  17. Serra Mauro Dalla, Menestrina Gianfranco, Carpaneto Armando, Gambale Franco, Fogliano Vincenzo and Ballio Alessandro (2003)., Molecular mechanisms of action of syringopeptins, antifungal peptides from Pseudomonas syringae pv. Syringae., CRC Press 203; 272-295. Print ISBN: 978-0-415-29852-0, eBook ISBN: 978-0-203-98664-6. https://doi.org/10.1201/9780203986646.ch13.
  18. Raaijmakers J.M., Vlami M. and Souza J.T. (2002)., Antibiotic production by bacterial biocontrol agents., Antonie Van Leeuwenhoek, 81, 537-547.
  19. Vogel A.I. (1968)., A text of quantitative analysis including elementary instrumental analysis., The English Lan Book soci & Long-mans, 3rd Ed: 180.
  20. Cartwright D.K., Chilton W.S. and Benson D.M. (1995)., Pyrrolnitrin and phenazine production by Pseudomonas cepacia, strain 5.5 B, a biological agent of Rhizoctonia solani., Appl Microbiol Biotech, 43(2), 211-116.
  21. Rosales A.M., Thomashow L., Cook R.J. and Mew T.W. (1995)., Isolation and identification of antifungal metabolites produced by rice-associated antagonistic Pseudomonas spp., Phytopatho, 85(9), 1028-1032.
  22. Winkelmann G. and Drechsel H. (1997)., Microbial siderophores. Biotechnology. HJ Rehm and G. Reed (eds.)., Second Edition. VCH, Weinheim, 7, 199-246.
  23. O’Sullivan D.J. and O’Gara F. (1992)., Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens., Microbiol Rev., 56(4), 662-676.
  24. Anjaiah V., Koedam N., Nowak-Thompson B., Loper J.E., Hofte M., Tambong J.T. and Cornelis P. (1998)., Involvement of phenazines and anthranilate in the antagonism of Pseudomonas aeruginosa PNA1 and Tn5 derivatives toward Fusarium spp. and Pythium spp. Mol Pla-Micro Inter., 11(9), 847-854.
  25. Vanitha S. and Samiyappan R. (2007)., Screening of bacterial antagonistic microorganisms under in vitro conditions against Alternaria chlamydospora causing leaf blight disease in Solanum nigrum L., Biomed., 2(2), 155-163.
  26. Adhikari A., Dutta S., Nandi S., Bhattacharya I., Roy M., de Sarkar G. and Mandal T. (2013)., Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani., Afri J of Agri Res., 8(4), 405-412.
  27. Crowe Jonathan D. and Olsson Stefan (2001)., Induction of laccase activity in Rhizoctonia solani by antagonistic Pseudomonos fluorescens strains and a range of chemical treatments., Appl Environ Microbiol, 67(5), 2088-2094.
  28. Gupta C.P., Sharma A., Dubey R.C. and Maheshwari D.K. (1999)., Pseudomonas aeuriginosa as a strong a strong anatagonis of Macrophomina phaseolina and Fusarium oxyporum., Cytobios, 99, 183-189.
  29. Gupta C.P., Bhavesh K., Dubey R.C., Maheshwari D.K. (2006)., Chitinase mediated destructive antagonistic potential of Pseudomonas aeruginosa GRC1 against Sclerotinia scleotiorum causing stem rot peanut., Bio Control, 51, 821-835. doi:10.1111/j.1365-2672.2004. 02252.x. J Appl Microbiol, 96, 1151-1160.
  30. Zehnder G., Murphy J.F., Sikora E.J. and Kloepper J.W. (2001)., Application of rhizobacteria for induced resistance., Eur J of Plant Patho, 107, 39-50.