6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Cytotoxic effects of Eupatorium odoratum on the Ultrastructure of the Midgut Epithelium of late instar larvae of Orthaga exvinacea Hampson

Author Affiliations

  • 1Department of Zoology, Malabar Christian College, Affiliated to the University of Calicut, Calicut, Kerala, India
  • 2Department of Zoology, Malabar Christian College, Affiliated to the University of Calicut, Calicut, Kerala, India

Int. Res. J. Biological Sci., Volume 5, Issue (3), Pages 26-30, March,10 (2016)


A study on the effect of methanolic leaf extract of Eupatorium odoratum on the ultrastructure of the midgut epithelium of sixth instar larvae of Orthaga exvinacea were carried under laboratory condition. Five different concentrations (1%, 2%, 3%, 4% and 5%) of botanicals were prepared. Among the five different concentrations of botanicals tested and by light microscopic studies, the highly effective 4% was selected for the ultrastructural study. The effects of E. odoratum were evidently showed in the ultrastructure of epithelial layer, mainly consisting of excessive vacuolation and elongation of columnar and goblet cells, detachment of epithelial cell from the basement membrane and the presence some secretory vesicles. Severe loss of microvilli were noticed in both inside the goblet cells and apical region of columnar cells. Reduced number of endoplasmic reticulum and shrunken columnar cell nucleus were observed. The active ingredients present in the leaf extract of E. odoratum might be the cause for the destructive changes in the midgut epithelium. Since these changes might lead to serious digestive and metabolic disorders, thereby affecting the growth of the larvae, this botanical can be used for the management of this pest.


  1. Singh R., Lakhanpal S.C. and Karkara B.K. (2006)., Incidence of mango leaf webber, Orthaga euadrusalis (Hampson) in high density plantation of mango at Dhaulakhan in Himachal Pradesh., Insect Environment, 11, 178-179.
  2. Kavitha K., Lakshmi K.V. and Anitha V. (2005)., Mango leaf webber Orthaga euadrusalis Walker (Pyralidae : Lepidoptera) in Andhra Pradesh., Insect Environment, 11(1), 39-40.
  3. Verghes E.A. (1998)., Management of mango leaf webber., A vitalpackage for panicle emergence. Insect Environment, 4(3), 7.
  4. Lamiri A., Lhaloui S., Benjilali B. and Berrada M. (2001)., Insecticidal effects of essential oils against hessian fly, Mayetiola destructor (Say)., Field Crops Research, 71, 9-15.
  5. Mukherjee U. and Singh H.N. (2006)., Eco-friendly approaches to manage Diamond- Back moth, Plutella xylostella (L.) in Cauliflower., Journal of Applied Zoological Research, 17(1), 57-60.
  6. Dwivedi S.C. and Karasawara K. (2003)., Larvicidal activity of five plant extracts against Culex quinquefasciatus., Indian Journal of Entomology, 65, 335.
  7. Rajamma P. (1982)., Effect of some organic materials on the control of sweet potato weevil Cylas formicarius Fab., Journal of Root Crops, 8(1-2), 64-65.
  8. Sreelatha K.B. and Geetha P.R. (2010)., Disruption of oocyte development and vitellogenesis in Oryctes rhinoceros treated with methanoloic extract of Eupatorium odoratum leaves., Journal of Biopesticide, 3(1), 253-258.
  9. Jing L., Fang Y., Ying X., Wenxing H., Meng X., Syed M.N. and Fang C. (2005)., Toxic impact of ingested Jatropherol-I on selected enzymatic activities and the ultrastructure of midgut cells in silkworm, Bombyx mori L., Journal of Applied Entomology, 129(2), 98–104.
  10. Barbera L.B., Alan T.M., Amanda D.G., David J.C. and Marilyn A.A. (2008)., Plant Cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae., Proceedings of the National Academy of Sciences of the United States of America, 105(4), 1221-1225.
  11. Sayed M.R. and Fayey A.B. and Mansour A.A. (2011)., Biochemical and histopathological effect of crude extracts on Spodoptera littoralis larvae., Journal of Evolutionary Biology Research, 3(5), 67-68.
  12. Salkeld E.H. (1951)., A toxicological study of certain new insecticides as “Stomach poisons” to the honey bee Apis mellifera., Journal of Canadian Entomology, 8339-8361.
  13. Terra W.R., Ferreira C. and Baker J.E. (1996)., Biology of Insect midgut. Lehane MJ and Billingsley PF (Eds.), Part two, Chapman and Hall, London, UK, 206-235.