Cytotoxic effects of Eupatorium odoratum on the Ultrastructure of the Midgut Epithelium of late instar larvae of Orthaga exvinacea Hampson
- 1Department of Zoology, Malabar Christian College, Affiliated to the University of Calicut, Calicut, Kerala, India
- 2Department of Zoology, Malabar Christian College, Affiliated to the University of Calicut, Calicut, Kerala, India
Int. Res. J. Biological Sci., Volume 5, Issue (3), Pages 26-30, March,10 (2016)
A study on the effect of methanolic leaf extract of Eupatorium odoratum on the ultrastructure of the midgut epithelium of sixth instar larvae of Orthaga exvinacea were carried under laboratory condition. Five different concentrations (1%, 2%, 3%, 4% and 5%) of botanicals were prepared. Among the five different concentrations of botanicals tested and by light microscopic studies, the highly effective 4% was selected for the ultrastructural study. The effects of E. odoratum were evidently showed in the ultrastructure of epithelial layer, mainly consisting of excessive vacuolation and elongation of columnar and goblet cells, detachment of epithelial cell from the basement membrane and the presence some secretory vesicles. Severe loss of microvilli were noticed in both inside the goblet cells and apical region of columnar cells. Reduced number of endoplasmic reticulum and shrunken columnar cell nucleus were observed. The active ingredients present in the leaf extract of E. odoratum might be the cause for the destructive changes in the midgut epithelium. Since these changes might lead to serious digestive and metabolic disorders, thereby affecting the growth of the larvae, this botanical can be used for the management of this pest.
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