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Study of Swarming Motility and pilB Gene among some Clinical isolates of Pseudomonas aeruginosa infections

Author Affiliations

  • 1Department of Soil and Water, College of agriculture University of Sallahaddin Erbil,Kurdistan,Iran

Int. Res. J. Biological Sci., Volume 5, Issue (2), Pages 23-29, February,10 (2016)

Abstract

This study was conducted to investigate the relationship between the swarming motility and pilB gene, twenty seven isolates of Pseudomonas aeruginosa were identified according to morphologicl, cultural and biochemical characteristics ,the isolates were from blood, Urine and Burn (nine isolates form each infection source). The sensitivity test of these isolates to the antibiotics was showed that all isolates were resistant of the used antibiotics except Amikacin, Tobramicin and Flucloxacillin. The ability of these isolates for swarming were tested the result showed that all isolates were able to swarming (dendritic swarming pattern). There was differences in swarming shapes among same infection and different source of infections on the semisolid surface (0.5-1)% agar plates, then these isolates were tested to evaluated the present pilB gene (type IV fimbrial biogensis protein) by PCR technique using specific primers, the result of PCR showed that this gene was moderately distribution (51.8)% in all studied isolates, since the result showed that this gene was widely disseminated in urine isolates 8/9(89)% while was 3/9(33.3)% in each of blood and burns isolates. These results clearly favored conclusion that differences swarming shapes in Pseudomonas aeruginos a from isolate to another may be the isolates have difference virolence factors for infection and it is important in all types of infections while prevalence of pilB gene perhaps related to infection site, there are no relationship between pilB gene presence and swarming motility.

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