International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Isolation of Beneficial Microbes for the Biodegradation of Animal and Plant Oil in Fast Foods Wastewater Effluents

    , , , , ,

Author Affiliations

  • 1Environment and Biotechnology Division, Industrial Technology Development Institute, Department of Science and Technology,
  • 2General Santos Avenue, Bicutan Taguig City 1631 Metro Manila, PHILIPPINES

Int. Res. J. Biological Sci., Volume 4, Issue (9), Pages 10-16, September,10 (2015)

Abstract

This study on the isolation and screening of aerobic microbes was developed for the treatment of fast foods wastewater effluents to reduce the oil and grease processed under aerobic conditions. The microorganisms were isolated from different fast foods wastewater effluents having both high oil and grease content, a big problem in the environment. From an initial 130 selected isolates, 54 were screened, then 28; thereafter eleven (11) isolates were found the most promising. The isolated microbes then identified as bacteria were screened and applied to the effluent and simulated wastewater for bioprocess treatment. These microbes were inoculated in nutrient broth for 24 hours at room temperature in an environmental shaker. These cultures serve as inoculum for treating contaminated fast foods wastewater in the laboratory for twenty four (24) hours. Analytical results showed that the highest reduction of oil and grease had 93.75% for the isolate JB8/8 (uncultured Basidiomycota) in wastewater effluents and isolate JB4/6 (Bacillus cereus) was 98.25 % in simulated wastewater.

References

  1. Huntley M.E., Biotreatment of Agricultural Wastewater. CRC Press (1989)
  2. Akpor O.B., Wastewater Effluent Discharge: Effects and Treatment Process. Proceedings 3rd International Conference on Chemical, Biological and Environmental Engineering (IPCBEE), 20, 85-91 (2011)
  3. Keener K.M., Ducoste J.J. and Holt L.M., Properties influencing fat, oil, and grease deposit formation, Water Environment Research, 80, 2241-2246 (2008)
  4. Parco G. F. and Kanzler A., Engineered Reed Bed Treatment System as a Low Cost Sanitation Option for the Philippines, UNDP Philippines Country Meeting the MDG (2002)
  5. Unpublished observation, in Coronado F.F. et al., Isolation of beneficial microbes for the biodegradation of animal and plant oil in fast foods wastewater effluents, Terminal Report, Regular Project ITDI (2013)
  6. Kurian R. and Nakhla G., Performance of aerobic MBR treating high strength oily wastewater at mesophilic–thermophilic transitional temperatures, Proceedings of the Water Environment Federation, 2006(9), 3249-3255 (2006)
  7. Barnes K.H., Meyer J.L. and Freeman B.J., Georgia Water Resources Conference, March 20-22, 1997, the University of Georgia, Athens Georgia (1998)
  8. National Small Flows Clearinghouse, High-Strength Flows-not Your Average Wastewater, Pipeline, Summer, 14(3) (2003)
  9. APHA, Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Healt Association (APHA), Washington DC (2005)
  10. Gonzalez C, Richard Greenwood and Philippe P., Quevauviller, Chemical and Biological Techniques for Water Monitoring, John Wiley and Sons (2009)
  11. Republic Act No. 9275, An act providing for a comprehensive water quality management and for other purposes, Philippine Clean Water Act of 2004, Congress of the Philippines (2004)m
  12. Li Y. and Migliaccio K. (Eds.), Water Quality Concepts,Sampling, and Analysis, (7), 137-156 USA. CRC Press(2011)