Optimization of Fermentation parameters for Enhanced production of Lipase from lipolytic Pseudomonas spp.
- 1Haffkine Institute for Training, Research and Testing, A.D.Marg, Parel, Mumbai 400 012, Maharashtra, INDIA
Int. Res. J. Biological Sci., Volume 4, Issue (6), Pages 16-21, June,10 (2015)
Lipases (Triacylglycerol acylhydrolases, EC 188.8.131.52) are enzymes which catalyze the enzymatic hydrolysis of triacylglycerol into diacylglycerol and fatty acids. Lipases are one of the most important enzymes that are used in various industries like Detergent, Food, Leather, Textiles, Pharmaceuticals, etc. Though various organisms used for the production and purification of lipase for industrial applications, Pseudomonas spp. remains considerably less explored. The present study thus explores the ability of Pseudomonas spp. isolated from oil contaminated soil samples for production of extracellular lipase enzyme. For the present study, 29 oil contaminated soil samples were collected from different regions of Pune, Solapur and Mumbai. Total 50 lipolytic Pseudomonas spp. were isolated by using Tributyrin agar medium containing Rhodamine B. Isolate showing maximum lipase activity (SP45) was then selected for further optimization studies. During optimization of fermentation parameters, SP45 showed optimum lipase activity of 50.4967 U/ml when Glucose was used as a carbon source, while lipase activity of 61.6567 U/ml was observed when Yeast extract was used as a nitrogen source. On optimizing the pH and incubation temperature of the fermentation medium, SP45 showed maximum lipase activity of 66.2567 U/ml in fermentation medium of pH 7at 37oC.
- Sharma R., Chisti Y. and Banerjee U., Production, purification, characterization, and applications of lipases, Research Review Paper, Biotechnol. Adv. 19, 627–662(2001)
- Verma N., Thakur S. and Bhatt A.K., Microbial Lipases: Industrial Applications and Properties, A Review, Int. Res. J. Biological Sci. 1(8), 88-92 (2012)
- Aravindan R., Anbumathi P. and Viruthagiri T., Lipase applications in food industry, A Review, Indian J. Biotechnol., 141-158 (2007)
- Sharma D., Sharma B. and Shukla A., Biotechnological Approach of Microbial Lipase, A Review, Biotechnology, 10(1), 23-40 (2011)
- Santhini K., Myla J., Sajani S. and Usharani G., Screening of Micrococcus Sp. from Oil Contaminated Soil with Reference to Bioremediation, Bot. Res. Int., 2(4), 248-252 (2009)
- Sirisha E., Rajasekar N. and Lakshmi Narasu M., Isolation and Optimization of Lipase Producing Bacteria from Oil Contaminated Soils, Advan. Biol. Res., 4(5), 249-252 (2010)
- Selva Mohan T., Palavesam A. and Immanvel G., Isolation and characterization of lipase-producing Bacillus strains from oil mill waste, Afr. J. Biotechnol., 7(15), 2728-2735 (2008)
- Pratt J., Cooley J., Purdy C. and Straus D., Lipase Activity from Strains of Pasteurella multocida, Curr. Microbiol., 40, 306–309 (2000)
- Amara A.A. and Salem S.R., Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa, Am-Euras. J. Agric. and Environ. Sci., 5(4), 556-563 (2009)
- Mishra A., Yaginik S., Mishra P. and Yadav S., Screening and Temperature Optimization for Lipase-Producing Bacteria from Waste Contaminated Water, Asian J. Biochem. Pharm. Res., 1(1), 62-69 (2011)