6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Toxicological Studies of Herbal Anti-Tumor Extract (Uvaria Chamae) in Monosodium Glutamate and Tamoxifen Treated Sprague-Dawley Rat

Author Affiliations

  • 1Public Health Division, Nigerian Institute of Medical Research, Lagos, NIGERIA
  • 2 Department of Chemical Sciences, Yaba College of Technology, Lagos, NIGERIA
  • 3 National Agency for Food and Drug, Administration and Control, Ilorin, NIGERIA
  • 4 Department of Biochemistry, College of Medicine, University of Lagos, Lagos, NIGERIA

Int. Res. J. Biological Sci., Volume 4, Issue (6), Pages 62-68, June,10 (2015)


Tumor cases are being linked to diet, lack of physical activity and overweight with Monosodium glutamate (MSG) and Tamoxifen (TAM) playing some roles. 45 female rats, weighing between 80g 120g were divided into 3 main groups. 20 of the rats received MSG of 150mg/kg body weight, another 20 rats received TAM of 20mg/kg body weight and the remaining 5 rats were the control group. This was done for 30 days after which the rats were divided into 4 subgroups and were treated for 21 days with ethanolic extract of Uvaria Chamae. They were then sacrificed, blood samples were collected into plain bottles and organs were harvested into universal bottle (fixed in 10% formal saline) for histopathology. It was observed that the amount of antibodies bound by the tumor markers was very significant in the MSG and TAM controls compared to the normal control (no tumor inducer rat). Administration of Uvaria Chamae extract was observed to cause very significant reduction in values of CA 15-3, CA-125 and CEA in both MSG and TAM groups compared to the untreated control. From the histopathology results, necrosis was observed in both treated and untreated groups of MSG and TAM but was not observed in the micrograph of the normal control. This study shows increase in the tumor markers values after induction with MSG and TAM, but Uvaria Chamae extract significantly reduced all values of the cancer tumor markers. The micrograph result showed damages done to the liver (by MSG and TAM), and this could not be corrected with Uvaria Chamaeextract.


  1. World Cancer Research Fund, WCFR (www.wcfr.org)., The Punch Newspaper, September 14th, (2011)
  2. Haran E.F., Maretzek A.F., Goldberg I., Horowitz A. and Degani H., Tamoxifen Enhances Cell Death in Implanted MCF7 Breast Cancer by Inhibiting Endothelium Growth, Cancer Res., 54, 55115514 (1994)
  3. Lindner D.J. and Borden E.C., Effects of tamoxifen and interferon-beta or the combination on tumor-induced angiogenesis, Int J Cancer., 71, 456461 (1997)
  4. Guo Y., Mazar A.P., Lebrun J.J. and Rabbani S.A., An Antiangiogenic Urokinase-derived Peptide Combined with Tamoxifen Decreases Tumor Growth and Metastasis in a Syngenetic Model of Breast Cancer, Cancer Res., 62, 46784684(2002)
  5. Dabrosin C., Margetts P.J. and Gauldie J., Estradiol Increases Extracellular Levels of Vascular Endothelial Growth Factor In Vivo in Murine Mammary Cancer, Int J Cancer., 107, 535540 (2003a)
  6. Dabrosin C., Palmer K., Muller W.J. and Gauldie J., Estradiol Promotes Growth and Angiogenesis in Polyoma Middle Transgenic Mouse Mammary Tumor Explants, Breast Cancer Res Treat., 78, 16 (2003b)
  7. Garvin S. and Dabrosin C., Tamoxifen Inhibits Secretion of Vascular Endothelial Growth Factor in Breast Cancer In Vivo, Cancer Res., 63, 87428748 (2003)
  8. Elkin M., Orgel A. and Kleinman H.K., An Angiogenic Switch in Breast Cancer Involves Estrogen and Soluble Vascular Endothelial Growth Factor Receptor 1, J Natl Cancer Inst., 96, 875878 (2004)
  9. Ates D.A. and Erdogrul O.T., Antimicrobial Activities of Various Medicinal and Commercial Plant Extracts, Turk, J. Biol., 27, 157-162(2003)
  10. Okogun J.I., Drug Production Efforts in Nigeria, Medicinal Chemistry Research and Missing Link, Being a Text of a Lecture given to the Nigeria Acad Sci., 29-52 (1985)
  11. Oliver B., Medicinal Plants in Tropical West Africa, Cambridge, 123-125 (1986)
  12. Irvin F.R., Woody Plants of Ghana with Special Reference to their Uses, Oxford University Press, London, 19, 20, 695 (1961)
  13. Okwu D.E. and Iroabuchi F., Classification of Uvaria Chamae, J.Chem Soc., Nigeria, 29(2), 112-114 (2004)
  14. Okwu D.E., Medicinal Properties of Uvaria ChamaeRoot, Stem and Leaves, Med Aromat Plant Sci Biotechnol., 1(1), 90-96 (2007)
  15. Bolarin D.M. and Bolarin A.T., Revision Note on Chemical Pathology, 1st Edn. Lantern Books, Lagos, 210-247, (2005)
  16. Farber J.L., Chein K.R. and Mittnacht S., The Pathogenesis of Irreversible Cell Injury in Ischemia, Am J. Pathol., 102, 271-281 (1981)
  17. Belluardo M., Mudo G. and Bindoni M., Effect of Early Destruction of the Mouse Arcute Nucleus by MSG on Age Dependent Natural Killer Activity, Brain Res., 534, 225-333 (1990)
  18. Martins L.J., Deobler J.A., Shih T. and Anthony A., Cytophotometric Analysis of Thalamic Neuronal RNA in some Intoxicated Rats, Life Sci., 35, 1593-1600 (1984)