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Optimization and Production of Alkaline protease from Bacillus subtilis IAS01 using agro-industrial by-product under SSF

Author Affiliations

  • 1Division of Microbiology, Faculty of Science, Annamalai University, Annamalai nagar-608002, Tamil Nadu, INDIA
  • 2Department of Microbiology, Faculty of Agriculture, Annamalai University, Annamalai nagar-608002, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (4), Pages 60-64, April,10 (2015)

Abstract

Edible and non-edible oil cake utilized for alkaline protease production by Bacillus subtilis IAS01 (KF 761633) in SSF. Experimentally revealed that groundnut oil cake and palm oil cake mixed in a ratio of 5:5 (w/w) supported for protease production when compared to individual and mixed substrate. In addition to attain the maximum yield of this enzyme in appropriate fermentation conditions viz. moisture 30%, 72 h incubation, 20% inoculum, pH 9.0 and temperature 37°C with recorded 835.13±5.04 units per gram of substrate. These results indicate that mutual inoculants of groundnut oil cake and palm oil cake are potential substrate for alkaline protease production at low quantities of substrate. In feature its best opportunity for protease production at industrial level.

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