International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Evaluation of Ultraviolet -B radiation induced changes in biochemical response of Arthrospira platensis (Gomont)

    , , ,

Author Affiliations

  • 1Centre for Advanced Studies in Botany, University of Madras, Chennai, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (4), Pages 52-59, April,10 (2015)

Abstract

In the current study, the influence of Ultraviolet - B radiation with intensity of 3 W/m on growth, biochemical changes and antioxidant enzymes in Arthrospira platensis were examined. The additional UV-B radiation vitally reduced the development of the cell, total protein, pigments (i.e. Total chlorophyll, carotenoids and c - phycocyanin). The study revealed that rise in exposure period of UV-B radiation caused poorer growth of the organism. The outcomes demonstrated that photosynthetic complex was the vital focus of UV-B radiation causing deprivation of photosynthetic pigments. It was observed with an increase in lipid peroxidation, in contrast with untreated control of A. platensis. The antioxidant enzymes showed improved activities of peroxidase, superoxide dismutase and except catalase in UV-B illuminated cells. The test organism neutralizes the effect of free radicals produced under UV-B stress through increased activities of these antioxidant enzymes. The outcome of the result recommends that A. platensis was impervious to UV-B radiation and the conceivable negative impact on the growth of A. platensis have been adequately adjusted with increment in these antioxidant enzymes.

References

  1. Wang G., Hu C., Li D., Zhang D., Chen K. and Liu Y., The response of antioxidant systems in Nostoc sphaeroides against UV-B radiation and the protective effects of exogenous antioxidants, 39, 1034-1042 (2007)
  2. Vaishampayan A., Sinha R.P., Hader D.P., Dey T., Gupta A.K., Bhan U. and Rao L., Cyanobacterial biofertilizers in rice agriculture, Bot Rev,67, 453-516 (2001)
  3. Gorton H.L. and Vogelmann T.C., Ultraviolet radiation and the snow alga Chlamydomonas nivalis (Bauer) Wille, Photochem. Photobiol., 77, 608-615 (2003)
  4. Sinha R.P., Singh N., Kumar A., Kumar H.D. and Hader D.P., Impacts of ultraviolet-B irradiation on nitrogen-fixing cyanobacteria of rice paddy fields, J. Plant Physiol.,150, 188-193 (1997)
  5. Villafane E.V., Sundback K., Figueroa F. and Helbling W., Photosynthesis in the aquatic environment as affected by UVR. In: Helbling, W., Zagarese, H. (Eds.), UV Effects in Aquatic Organisms and Ecosystems, The Royal Society of Chemistry, Cambridge, UK, 357-397 (2003)
  6. Hader D.P. and Sinha R.P., Solar ultraviolet radiation-induced DNA damage in aquatic organisms: potential environmental impact, Mut. Res. Fund. Mol.Mech. Mutag., 571, 221-233 (2005)
  7. Gao K., Li G., Helbling E.W. and Villafan V.E., Variability of UVR Effects on Photosynthesis of Summer Phytoplankton Assemblages from a Tropical Coastal Area of the South China Sea, 103, 802-809 (2007)
  8. Bennett A. and Bogorad L., Complimentary Chromatic Adaptation in a Filamentous Blue, Green Alga, The Jour of Cell Biol., 58, 419 (1973)
  9. Lichtenthaler H.K., Chlorophylls and carotenoids: pigments of photosynthetic biomembranes, In: Packer, L. and Douce, R. eds., Methods in Enzymology, Washington, Academic Press, 148, 350-382 (1987)
  10. Bradford M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding, Anal Biochem, 72, 248-254 (1976)
  11. Dubois M., Giles K.A., Hamilton J.K., Robeus R.A. and Smith E., Calorimetric methods for determination of sugars and related substance, Anal Biochem.,28, 305-365 (1956)
  12. Bates L.S., Wadern R.P. and Teare I.D., Rapid estimation of free proline for water stress determination, Plant Soil., 39, 205-207 (1973)
  13. Heath R.L. and Packer L., Photoperoxidation on in isolated Chloroplasts, I. Stoichiometry of fatty acid peroxidation, Arch Biochem. Biophys., 125, 189-98 (1968)
  14. Kato M. and Shimizu S., Chlorophyll metabolism in higher plants, VII. Chlorophyll degradation in senesc- ing Tobacco leaves, phenolic-dependent peroxidative degradation, Can. J. Bot., 65, 729-735 (1987)
  15. Beauchamp C. and Fridovich I, Superoxide dismuttases: improved assays and an assay applicable to acrylamide gels, Anal. Biochem., 44, 276-287 (1971)
  16. Callaghan T.V., Bjorn L.O., Chemov Y., Chapin T., Christensen T.R, Huntley B., Ims R.A., Johansson M., Jolly D., Jonasson S., Matveyeva N., Panikov N, Oechel W., Shaver G., Elster J., Jonsdottir I.S., Laine K. Taulavuori K. and Zockler C., Responses to projected changes in climate and UV-B at the species level, Ambio., 33, 418-435 (2004)
  17. Bornman J.F., Target sites of UV-B radiation in photosynthesis of higher plants, J. Photochem. Photobiol., 145-148 (1989)
  18. Dohler G., Effect of UV-B radiation on the marine diatoms Lauderia annulata and Thalassiosira rotulagrown in different salinities, Mar. Biol., 83, 247-253 (1984)
  19. Sinha R.P., Lebert M., Kumar A., Kumar H.D. and Hader D.P., Disintegration of phycobilisomes in a rice field cyanobacterium Nostoc sp. following UV irradiation, Biochem. Mol. Biol. Int.,37, 697-706 (1995)
  20. Ehling-Schulz M., Bilger W. and Scherer S., UV-B-induced synthesis of photoprotective pigments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoccommune, J. Bacteriol., 1940-1945 (1997)
  21. Kaplan D., Christiaen D. and Arad S., Chelating properties of extracellular polysaccharides from Chlorella sp. Appl. Environ. Microbiol., 12, 2953-2956(1987)
  22. Tripathi B.N. and Gaur J.P., Relationship between Copper and Zinc: Induced Oxidative Stress and Proline Accumulation in Scenedesmus sp., Planta, 219, 397-404 (2004)
  23. Girotti A.W., Photodynamic lipid peroxidation in biological systems, Photochem. Photobiol., 51, 497-509(1990)
  24. Chris A., Zeeshan M., Abraham G. and Prasad S.M., Proline accumulation in Cylindrospermum sp., Environ Exp Bot. 57, 154-159 (2006)