International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

In Vitro Rapid Multiplication of Wrightia Tomentosa (ROXB.) Roem. and Schultz an Endangered Medicinal Tree Species

    , , , ,

Author Affiliations

  • 1Plant Biotechnology Research Lab, Department of Biotechnology, Kakatiya University, Warangal, 506009, AP, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (3), Pages 10-14, March,10 (2015)

Abstract

The protocol for in vitro multiple shoot development and plantlet formation was developed using cotyledonary node and shoot tip explant in Wrightia tomentosa (Roxb.) Roem. and Schultz an endangered medicinally important forest tree. The explants were cultured on ¼ strength MS medium supplemented with various concentrations of BAP. Multiple shoots were induced from the cotyledonary nodal segments and shoot tip explants in all the concentrations of BAP. More percentage of multiple shoot induction was observed in cotyledonary nodal explants compared to shoot tips. Maximum number of shoots proliferation (12±1.07) with high frequency of shooting response was found in shoot tip explants followed by nodal explants at 3.0 mg/L BAP. The micro-shoots were cultured on ¼ strength MS medium with various concentrations of auxins IAA/IBA for in vitro rooting. Profuse rhizogenesis (16.2±2.01, 12.8±1.29 cm) was observed on ¼ strength MS medium supplemented with 1.0 mg/L IAA/1.5 mg/L IBA. The in vitro rooted plantlets were transferred to polycups containing sterile soil: vermiculite (1:1) and hardened in the culture room. These acclimatized plants were transferred into field and the survival percentage was found to be 75. These in vitro regenerated plants were similar to donor plant.

References

  1. Suchitra B., Mehar Z. and Susil K., In vitro multiplication of Centella asiatica, Curr. Sci., 76, 147-158 (1999)
  2. Boro P.S., Sharma Deka A.C. and Kalia M.C., Clonal Propagation of Alternanthera Sessilis: A biopharmaceutically potent herbal medicinal plant, J. Phytol. Res., 11, 103-106 (1998)
  3. Cassel A.C., Walsh C., Belin M., Cambornac M., Rohit J.R. and Lubrano C., Establishment of plantation from micropropagated Arnica chamissonsis a pharmaceutical substitute for the endangered A.montana, Plant Cell Tissue and Organ cult., 156, 139-144 (1999)
  4. Nepovim A. and Vanek T., In vitro propagation of Stevia rebaucliena plants using multiple shoot culture, Plant Med., 64, 775-776 (1998)
  5. Villalobos V.M. and Engelmann F., Ex situ conservation of plant germplasm using biotechnology, World J. Microb. Biotech., 11, 375-382 (1995)
  6. Tiwari V., Singh B.D. and Tiwari K.N., Shoot regeneration and somatic embryogenesis from different explants of Brahmi (Bacopa monniera (L) Wettst), Plant Cell Rep., 17, 538-543 (1998)
  7. Pillai S. K. and Sinha H.C., In statistical methods for biological works Ramprasad and Sons, Agra, (1968)
  8. Martin K.P., Rapid propagation of Holostemma adakodien Schult., a rare medicinal plants, through axillary bud multiplication and indirect organogensis, Plant Cell rep., 21, 112-117 (2002)
  9. Mulwa R.M.S. and Bhalla P.L., In vitro shoot multiplication of Macadancia tetrophylla, L. Johonson, J. Hortic Sci Biotechnol., 75, 1-5 (2000)
  10. Tornero O.P., Lopez J.M., Egea J. and Burgos L., Effect of basal medium and growth regulators on in vitro propagation of apricol (Prunus armenica) CV. Canino, J Horic sci Biotechnol.,75, 283-286 (2000)
  11. Krishna Pandey., Kavindra Nath Tiwari., Jayanti Singh., Jay Prakash Verma. and Satya Deo Dubey., In vitro propagation of Clitoria terrnatae L. A rare medicinal plant, J. Medici. Plant Resea.,4(8), 664-668 (2010)
  12. Wang J., Seliskar D.M. and Gallagher J.L., Plant regeneration via somatic embryogenesis in the brackish wetland monocot Seripus robostus, Aquatic Bot.,79, 163-174 (2004)
  13. Espinosa A.C., Pijut P.M. and Micher C.H., Adventitions shoot regeneration and rooting of Prunus scrotina in vitro culture, Hortic. Sct.,4,193-201 (2006)
  14. Sasikumar S., Ravinder S., Premkumar S., Ignarimuthu S. and Agastian P., Micropropagation of Baliospermum montanum (wild.) Mucll. Arg.-A threatened medicinal plant, Indian J Biotechnol., 8, 223-226 (2009)
  15. Yadav V., Lal M. and Jaiswal V.S., Micropropagation of Morus niger from shoot tip and nodal explants and mature trees, Sci Hortic.,44, 61-64 (1990)
  16. Veltchera M.R. and Svetleva D.L., In vitro regeneration of Phaseolus vulgaris L. Via organogenesis from petiole explants, J. Central Eurp. Agric.,6, 53-58 (2004)
  17. Rkhis A.C., Maalej M., Messooud S.O. and Drira N., In vitro regenerative growth and flowering of olive tree in response to GA3 treatment, Aft. J.Biotecho., 5, 2096-2302 (2006)
  18. Gasper T. and Coumams M., Root formation. In cell and tissue culture in foresty. Vol. II Bong, J.M. and Durzan, D.J. (Eols). Marhinus Nijh of publishers Dordrecht, 202-207 (1987)
  19. Purohit D. and Dave A., Micropropagation of Sterculia urens Roxb. An endangered tree species, Plant Cell Rep.,15, 704-706 (1996)
  20. Shahin-uz-zaman M., Ashratuzzaman M., Shahidul Haque M. and Lutafan Nahar luna., In vitro clonal propagation of the neem tree (Azadirachta indica) A.Juss, Afr.J.Biotechnol., 7(4), 386-391 (2008)
  21. Arya D., Patni V. and Kant U., In vitro propagation and querectin quantification in callus cultures of Rasna (Pluchea lanceolate Oliver and Hiern), Indian J. Biotechnol., 7, 383-387 (2008)